Cell Bio II Flashcards
Integral Proteins
Can go either all the way across the membrane, or just partway
Peripheral Proteins
Can be removed from the plasmalemma with salt solutions
Symptoms of Creutzfeldt-Jakob disease
ataxia, dementia, paralysis
Lab results in Creutzfeldt-Jakob disease
spongiform change in cerebrum and kuru plages of abnormal aggregates in cerebellum. Can use PAS or congo red to see
How does Creutzfeld-Jakob disease happen
- Normal prion gets converted to abnormal form
- Abnormal makes cross-linked filaments that are resistant to proteolysis
- Stimulates production of more abnormals over wild type
Functions of membrane proteins
Pumps and carries, channels, receptors, linkers, enzymes, and structural proteins
Na+/K+ATPase Pump
cleaves ATP to transports Na+ out of the cell and K+ into the cell. Regulates intracellular volume
Digoxin
Partially inhibits the Na+/K+ pump, leading to decrease in the activity of Ca/Na transporter which increases sacroplasmic Ca ion concentration, which improved cardiac pump performance
Co-transport of glucose and Na
- example of secondary active transport
- Does not require ATP
- Na+ and glucose are co-transported into the cell as Na moves down it’s conc. gradient
Multidrug resistant transports
subfamily of primary transport proteins that are ATPases
MDR-1
expressed in kidny, liver, intenstine and blood-brain barrier. Transports drugs
MDR-2
transports conjugated (direct) bilirubin
MDR-3
expressed in liver. Flippase of phosphatidylcholine
Dubin-John Syndrome
- Caused by mutation in ATP binding region of MDR-2 Protein.
- Impaired transcription and mislocation of the MDR-2 protein
Cystic Fibrosis
-mutation in CFTR, which is a chloride channel that regulates Na+ and HCO-3. Causes salty sweat, viscous secretion in intestine, pancreas, bile duct, and airways.
