Boo boo testing

Question 1

The reason, specimen type, reagents, reference range and results for: Platelet Count

Answer 1

Reason: To determine plt
amount

Specimen: WB

Reagents: n/a

RR: 150-400 x 10^3/mm^3

Results: <150x10^3 = thrombocytopenia

Question 2

The reason, specimen type, reagents, reference range and results for: Capillary fragility test

Answer 2

Reason: Assess plt-vessel wall interaction

Specimen: None

Reagents: none

RR: No petechiae formation

Results:
1+ = few
2+ = many
3+ = multi arm and hand
4+ = many arm and hand

Question 3

The reason, specimen type, reagents, reference range and results for: Bleeding time

Answer 3

Reason: Detect congenital and acquired disorders of plt function

Specimen: none - cut the forearm

Reagents: Sphygmomanometer

RR: 2.5 - 9.5 min

Results: > 9.5 min

Question 4

The reason, specimen type, reagents, reference range and results for: Platelet Function Analyzer (PFA) “Closure Time)

Answer 4

Reason: Simulates in vivo process of plt adhesion, activation, and aggregation

Specimen: WB

Reagents: Cartridge with collagen/ADP or Collagen/EPI

RR:
Col/ADP = 94.193 sec
Col/EPI = 71-118sec

Results:
Platelet defect/vWD = prolonged for both

Aspirin/Drug effect = Normal ADP, prolonged Epi

Question 5

The reason, specimen type, reagents, reference range and results for: Platelet Aggregation Studies

Answer 5

Reason: Determine aggregation pattern with light transmission

Specimen:

Reagents: Aggregating agent

RR: Normal depends on aggregating agent

Results:

Question 6

The reason, specimen type, reagents, reference range and results for: vWF:Ag

Answer 6

Reason: Determine aggregation pattern with light transmission

Specimen:

Reagents: Latex beads coated with monoclonal ab directed at GP1b binding domain

RR: If vWF is present, latex particles with agglutinate, decreasing turbidity

Results: No decrease in turbidity

Question 7

The reason, specimen type, reagents, reference range and results for: Ristocetin Cofactor (vWF:Rco)

Answer 7

Reason: Ability of plasma with vWF to agglutinate

Specimen: Plasma and donor or lyophilized plt

Reagents: Latex beads coated with recombinant GP1b

RR: If vWF is present, beads will agglutinate and decrease turbidity

Results: No decreased turbidity

Question 7

The reason, specimen type, reagents, reference range and results for: RIPA

Answer 7

Reason: Aggregation study

Specimen: PRP

Reagents: Ristocetin and NP plasma

RR: Normal agglutination

Results: Normal wioth all except ristocetin - NP plasma will correct

Question 7

The reason, specimen type, reagents, reference range and results for: Heparin Induced Thrombocytopenia testing

Answer 7

Reason: Test for the presence of heparin induced antibody

Specimen: PPP (off heparin for 8 hours)

Reagents: Platelet aggregometer, heparin dilutions, donor plts

RR: No aggregation

Results: Platelet aggregation
<20%T = undetermined
20-40%T = weak pos
>40%T = pos Ab

Question 8

Common agonists or aggregating agents: Von Willebrands

Answer 8

Ristocetin - corrects with NP plasma

Question 9

Common agonists or aggregating agents: Bernard Soulier

Answer 9

Ristocetin

Question 10

Common agonists or aggregating agents: Glanzmann’s Thrombasthenia

Answer 10

All abnormal except ristocetin

Question 11

Common agonists or aggregating agents: Delta Storage Pool Disease

Answer 11

ADP

Question 12

Common agonists or aggregating agents: Gray Platelet

Answer 12

ADP

Question 13

Common agonists or aggregating agents: Aspirin or NSAID therapy

Answer 13

Arachidonic acid curve = more sensitive