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Blood Cold Chain Flashcards

1
Q

A process from collection that begins at the time of collection and continues until the unit is transfused

A

Blood Cold Chain

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2
Q

Number 1 link in blood cold chain

A

Collection

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3
Q

A transport box which maintains the cold/controlled environment?

A

Chest Box

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4
Q

The temperature maintained after collection and during transport to the blood center?

A

Temperature: 20C - 24C

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5
Q

Ice chest components

A

Cold dogs/Ice packs

Insulated Cardboard

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6
Q

True or False.

Blood must be in Direct Contact with ice to prevent hemolysis

A

False. Blood must not be in direct contact with ice.

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7
Q

What is the result when blood units have direct contact with ice packs/cold dogs?

A

Hemolysis

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8
Q

Purpose of maintaining cold temperature after collection until processing?

A

Maximum clotting factors and platelets can be harvested from the donations

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9
Q

Time required for the transport of pre-processed specimen?

A

Within 8 hours

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10
Q

True or False. Untested specimen should NOT be mixed with tested specimen and separately stored untested specimen.

A

True

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11
Q

True or False. Time of Donation to time of arrival must exceed 8 hours

A

False

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12
Q

Temperature required from the arrival of processed blood units?

A

Temperature: 2C - 10C

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13
Q

Time required for blood units in the sink/bench and is limited to labeling, packing, and testing purposes.

A

Time on bench

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14
Q

Blood units must be safely stored at what temperature before the 24 hrs limit is reached?

A

4C

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15
Q

An equipment that provide continuous motion, gentle horizontal motion to the packs of this blood unit because this blood component easily disintegrate?

A

Platelet agitator

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16
Q

Temperature set by refrigerator centrifuge to maintain a cold chain temperature?

A

2C - 6C

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17
Q

Blood bag that separates blood into three components?

A

Triple blood bag

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18
Q

What are the components separated by the triple blood bag?

A

RBCs
Plasma
Platelets

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19
Q

Blood bag that is only used for transfusion and preservation of whole blood?

A

single blood bag

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20
Q

True or False. In platelet preparation, it begins with a heavy spin at temperature of 20C - 24C.

A

False. Light spin

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21
Q

What is light spin?

A

weak centrifugal force and short centrifugal time

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22
Q

RPM of Light Spin

A

2500-4200

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23
Q

How much volume is remained in the satellite bag to resuspend your platelet concentrate?

A

50 - 70 mL

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24
Q

What is the purpose of a weak centrifugal force and short centrifugal time in platelet preparation?

A

To preserve buffy coat, which platelets will be obtained.

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25
RPM of Heavy spin?
4700 - 5200
26
What should platelet concentrate demonstrate on the side of satellite bag after centrifugation by heavy spin?
Platelet ribbon
27
Why does platelet concentrate should be left stationary with label side down?
For gas exchange
28
Color formed by a platelet ribbon?
yellow or off white
29
Time required for a heavy spin?
7-12 minutes
30
It suggest viability of platelet concentrate and is demonstrated by the presence of a moving varied color before the unit is issued?
Swirling Phenomenon
31
Indices of a good platelet yield?
Platelet ribbon | Swirling phenomenon
32
When are platelet concentrates used?
actively bleeding patients Hemorrhage/Thrombocytopenic Cancer patients Thrombocytopenic pre-operative patients
33
Prepared from whole blood that begins with a heavy spin @ 4C?
Fresh Frozen Plasma
34
In FFP, the plasma labeled side down is placed at what temperature?
-18C or colder
35
True or False. Cryoprecipitate AHF is prepared from whole blood.
False. Prepared from Fresh Frozen plasma
36
In cryoprecipitate preparation, the the FFP is thawed at what temperature and timeframe?
2C - 6C; overnight
37
After overnight thawing of FFP and before doing heavy spin, what should be demonstrated by the plasma?
Mushy appearance
38
Why is cryosupernate separated to the cryoprecipitate that adheres in the blood bag?
To prevent the cryoprecipitate from dissolving and flowing out of the bag.
39
Volume of supernatant plasma remained to resuspend your cryoprecipitate in the bag?
15 - 30 mL
40
Time to refrozen cryoprecipitate?
within 1 hour
41
Cryoprecipitate is stored at what temperature and maximum storage time?
-30C for up to 12 months from the date of collection not from the date it was prepared
42
Difference of cryoprecipitate to FFP when thawed?
Temperature is raised to 37C in water bath before it is issued.
43
Packed RBCs used what type of blood bag?
Double blood bag
44
How much volume is removed to obtain an 80% hematocrit in packed RBC preparation?
20%
45
Blood is processed to prevent febrile transfusion reaction that removes WBCs, what type of blood processing is asked?
Leuko-reduced packed RBCs
46
What causes febrile transfusion reaction?
If blood is not processed within 24 hrs that results to the formation of cytokines
47
Methods used in Lueko-reduced packed RBCs
Filtration | Microcentrifugation
48
A blood component that undergo the process of irradiating/inactivating T lymphocytes to prevent GVHD among immunocompromised and firs degree relative?
Irradiated Blood Component
49
Expiration date of Packed RBCs
28 days from preparation or unit of expiration
50
Expiration date of PC?
5 days (associated to its shelf life)
51
Links in the Blood Cold Chain
``` Collection Processing Storage Delivery Transfusion ```
52
Why WB are stored at low temperature (2C - 6C)?
To prevent bacterial growth and minimize cell metabolism
53
Causes of bacteria in Blood Bag
``` Poor aseptic technique/disinfection Transient bacteremia (air inside the blood bag) ```
54
Leading cause of bacterial contamination
Air inside the blood bag
55
Storage and Transport of WB and Red Cells
Transport (Pre-processed): 20 - 24 C - within 8 hrs Storage (Pre-post processing): 2 - 6 C - 35 days Transport (Processed): 2 - 10 C - within/< 24 hrs
56
Documents form upon ISSUANCE
``` Name of Patient Name of Facility Serial Number Expiration date Temperature upon release Time of blood unit was sent ```
57
Documents form upon RELEASE
Time received Temperature upon received Serial Number Blood Evaluation (Quality)
58
Required temperature of PC
20C - 24C
59
True or False. Platelets have higher risk for bacterial contamination due to its storage temperature.
True
60
Time required for the separation of FFP from whole blood?
6 hrs - 8 hrs
61
A protein that is stable at 2C - 6C?
Albumin
62
Clotting factors that are labile and must be stored @ -20C or lower?
PF V & VIII
63
Isolate of Cryo plasma contains this plasma factors?
Factor I (Fibrinogen) Factor VIII (AHF) vWF Factor XIII
64
It is used to store components of blood from FFP and Cryoprecipitate?
Ultralow Freezer
65
What is the good circulation of air between pieces of equipment?
30 cm
66
Good circulation of air from the ceiling?
40 cm
67
Paper Test
Evaluates the equipment if the cold air escapes and if not sealed properly
68
Significance of validated containers?
Withstand hot & cold ambient conditions For temperature is maintained for a period of time long enough to complete delivery/transport. Prevent tampering and robust enough not to break open on impact
69
Done prior and after delivery
Temperature monitoring
70
What is ideal issuance? Its purpose?
Issue one unit at a time; To avoid issuing wrong blood units to the patient.
71
Once unit is not used, it should be returned/reiisued after how many minutes?
Within 30 minutes
72
What is done when reissuance of blood unit exceeded 30 minutes?
DISCARDED
73
What should be performed before transfusion?
Blood warming Plasma thawing User education
74
Only institution that performs irradiated blood component?
National Kidney and Transplant Institute
75
What indicates a greenish blood unit of prepared platelets/
bacterial contamination
76
True or False. Turbid blood unit indicates a non-hemolyzed blood
False. Unit (platelet) starts to became turbid after 24 hrs as it starts to hemolyzed.
77
Rule in method of storage of available stock?
FIRST IN, FIRST OUT
78
True or False. The strength of the chain depends on the strength of the weakest link, not the strongest one..
True

IHBB (11 decks)

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