Biology Midterm 2 Flashcards
Interphase
The period from cell formation to cell division - Carrying out all routine activities
G1
Cell cycle subphase: Metabolically active, synthesizing proteins and rapid growth
S phase
Cell cycle subphase: DNA is replicated
G2
Cell cycle subphase: brief final phase of interphase; prepares for cell division (centriole replication complete
Replication bubble
Bubble formed after enzymes unwind DNA
Replication fork
The point at which DNA strands unzip
DNA polymerase
The enzyme that travels along the DNA template strands, forming two new complementary strands
Semi conservative replication
Each new DNA molecule consists of one old and one new nucleotide strand
M (mitotic phase)
Cell division: Involves mitosis and cytokinesis
Mitosis
The division of the nucleus (cell division)
Cytokinesis
The division of the cytoplasm
Cleavage furrow
A furrow that forms during cytokinesis to pinch off the two new daughter cells
mRNA (messenger RNA)
Nucleotide strands resembling “half-DNA’ molecules (one of two DNA strand complementary molecules for protein structure)
rRNA
Forms ribosomes (consists of two subunits - one large, one small)
Transcription
Transfers information from a DNA base sequence to complementary mRNA
Promoter
Special DNA sequence that contains the start point of a gene to be transcribed
RNA polymerase
The enzyme that oversees the synthesis of mRNA
Translation
Translates mRNA to proteins (from base sequence to amino acid sequence)
Genetic code
Rules by which the base sequence of a gene is translated into an amino acid sequence
Codon
The corresponding three-base sequence on mRNA for each DNA triplet
Anticodon
A three-base sequence, which binds to the mRNA codon calling for the amino acid carried by that particular tRNA
Initiation
Ribosomal subunit binds to initiator tRNA and scans mRNA for the start codon (AUG)
Elongation
Ribosomes moves along mRNA in one direction, generating amino acids forming the polypeptide chain
Elongation steps
a. Codon recognition
Aminoacyl-tRNA binds to complementary codon in A site of ribosome
b. Peptide bond formation
Enzymatic component in large ribosomal subunit catalyzes peptid bond formation between the amino acid of the tRNA in the P site and that of the tRNA in the A site
c. Translocation
The ribosome moves, shifting its position one codon along the mRNA. This shift moves tRNA in the A site to the P site. Vacant tRNA is transferred to the E site to be released and recharged with another amino acid.
Termination
mRNA strand is read sequentially until the stop codon (UGA, UAA, or UAG) enters the A site. Signals that translation of mRNA is finished and water is added to the polypeptide chain to break the bond between the chain and the tRNA in the P site. The completed polypeptide chain is then released from the ribosome
ER signal sequence
Short “leader” peptide - When present in a protein being synthesized the associated ribosome attaches to the membrane of the rough ER
MicroRNAs (miRNAs)
Small RNAs that can interfere with and suppress mRNAs made by certain exons, effectively silencing them
Small interfering RNAs (siRNAs)
Like miRNA but originate from outside the cell
Autophagy
(self-eating) Sweeps up bits of cytoplasm and unneeded organelles into double-membrane vesicles called autophagosomes. They are then delivered to lysosomes for digestion of contents which cell reuses
- Allows cells to dispense of unneeded proteins and worn out organelles
- In times of stress can cannibalize to survive
- Helps to restructure the cells
Ubiquitin-proteasome pathway
Handles individual proteins that are misfolded, damaged, or unneeded and must be disposed of
Ubiquitins
Markers for proteins needing destruction
Proteasomes
Waste disposal complexes composed of protein-digesting enzymes
Apoptosis
Programmed cell death; rids the body of cells that are programmed to have a limited life span (cells in uterus and webs between fingers and toes of developing fetus)
Hyperplasia
Accelerated growth; in anemic people this results in accelerated growth of red blood cells
Atrophy
A decrease in size of an organ or body tissue
Tissues
Groups of cells that are similar in structure and perform a common/related function
Histology
The study of tissues
Fixed
Preserved (as relating to microscopy)
Sections
Slices (as relating to microscopy)
Artifacts
Minor distortions in preserved tissue after it has been exposed to many procedures that alter its original condition (as relating to microscopy)
Epithelial tissue
Epithelium; A sheet of cells that covers a body surface or lines a body cavity
Covering and lining epithelium
Forms the outer layer of the skin; dips into and lines the open cavities of the urogenital, digestive, and respiratory systems; and covers the walls and organs of the closed ventral body cavity
Glandular epithelium
Fashions the glands of the body
Epithelium functions
1 Protection 2 Absorption 3 Filtration 4 Excretion 5 Secretion 6 Sensory reception
Apical surface
Not attached to surrounding tissue and is exposed to either the outside of the body or the cavity of an internal organ
Basal surface
Attached to underlying connective tissue
Apical-basal polarity
The polarity between the apical and basal surfaces
Microvilli
Finger like extensions of the plasma membrane to increase surface area
Basal lamina
Thin supporting sheet adjacent to basal surface of epithelium - Consists largely of glycoproteins secreted by epithelial cells + collagen fibers. Acts as selective filter determining which molecules diffusing from the underlying connective tissue are allowed to enter the epithelium. Also can act as scaffolding along which epithelial cells can migrate to repair a wound
Basement membrane
In between the epithelial and connective tissues that reinforces the epithelial sheet, helps it resist stretching and tearing, and defines the epithelial boundary
Consists of basal lamina and reticular lamina
Reticular lamina
Deep to the basal lamina: consists of a layer of extracellular material containing a fine network of collagen protein fibers that “belongs to” the underlying connective tissue
Simple epithelia
Consist of a single cell layer - typically found where absorption, secretion, and fitration occur and a thin epithelial barrier is desirable
Stratified epithelia
Composed of two or more cell layers stacked on top of each other; common in high-abrasion areas where protection is important, such as the skin surface and lining of the mouth
Squamous cells
Flattened and scale-like cells
Cuboidal cells
Boxlike cells, approximately as tall as they are wide
Columnar cells
Tall and column shaped cells
Simple squamous epithelium
Cells flattened laterally with a sparse cytoplasm - Allows materials to pass by diffusion and filtration in sites where protection is not important: kidney glomuleri, air sacs of lungs, lining of heart, blood vessels etc
Endothelium
Provides a slick, friction-reducing lining in lymphatic vessels and in all hollow organs of the cardiovascular system; Cpillaries - efficient exchange of nutrients and wastes between the bloodstream and surrounding tissue cells
Mesothelium
The epithelium found in serous membranes, the membranes lining the ventral body cavity and covering its organs
Simple cuboidal epithelium
Consists of a single layer of cells as tall as they are wide - secretion and absorption. Forms the walls of the smallest ducts of glands and many kidney tubules
Simple columnar epithelium
Single layer of tall, closely packed cells, aligned like soldiers in a row; lines the digestive tract from the stomach through the rectum. Mostly associated with absorption and secretion and the digestive have 2 modifications: Dense microvilli on apical surface of absorptive cells 2 Tubular glands made of cells that secrete mucus-containing intestinal juice
Psuedostratified columnar epithelium
Vary in height; all cells rest on basement membrane but only the tallest reach the free surface of the epithelium. Because cell nuclei lie at different levels above the basement membrane, the tissue gives false (pseudo) impression that several layers are present. Short cells relatively unspecialized and give rise to the taller cells. Secretes or absorbs substances; ciliated version containing mucus-secreting goblet cells lines most of the respiratory tract
Stratified squamous epithelium
Most widespread of the stratified epithelia. Composed of several layers, it’s thick and well suited for protection. Free surface cells are squamous and cells of deeper layers are cuboidal or columnar. This epithelium forms the external surface of the skin and exends a short distance into every body opening that is directly continuous with skin (outer layer of epidermis is keratinized for tough protection)
Stratified cuboidal epithelium
Quite rare mostly found in the ducts of some of the larger glands
Stratified columnar epithelium
Limited distribution in the body. Small amounts found in the pharynx, male urethra, and lining some glandular ducts. This epithelium also occurs at transition areas or junctions between two other types of epithelia. Only its apical layer of cells is columnar
Transitional epithelium
Forms the lining of hollow urinary organs, which strech as they fill with uring. Cells of its basal layer are cuboidal or columnar. Apical cells vary in appearance, depending on the degree of distension (stretching of the organ)
Gland
Consists of one or more cells that make and secrete a particular product
Secretion
An aqueous fluid that usually contains proteins
Endocrine glands
Lose their ducts during development (ductless glands) Produce hormones
Hormones
Chemical messengers that they secrete by exocytosis directly into the extracellular space. Travel to specific target organs
Exocrine glands
Secrete their products onto body surfaces or into body cavities. Unicellular glands do so by exocytosis whereas mutlicellular glands do so via an epithelium-walled duct that transports the secretion to the epitheliual surgace
Unicellular glands
Mucous cells and goblet cells. Sprinkled in the epithelial linings of the intestinal and respiratory tracts amid columnar cells with other functions
Mucin
A complex glycoprotein that dissolves in water when secreted. Once dissolved, mucin forms mucus, a slimy coating that protects and lubricates surfaces
Goblet cells
Accumulating mucin distends the top of the cell, making the cells look like a glass with a stem
Multicellular exocraine glands
Structurally complex with two basic parts: epithelium derived duct and secretory unit (acinus), consisting of secretory cells. In all but the simplest glands, supportive connective tissue surrounds the secretory unit, supplies it with blood vessels and nerve fibers, and forms a fibrous capsule that extends into the glands and divides it into lobes
Simple glands
Have an unbranched duct (classification of glands)
Compound glands
Have a branched duct (classification of glands)
Tubular
Secretory cells form tubes (categorization of glands by secretory units)
Alveolar
Secretory cells form small, flasklike sacs (categorization of glands by secretory units)
Tubuloalveolar
Both types of secretory units (categorization of glands by secretory units)