BIOLOGY/BIOCHEM QUESTIONS Flashcards

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1
Q

Name two possible sources of error when estimating Km and Vmax from a Lineweaver-Burk plot.

A
  1. The inability to obtain a negative value of 1/[S] often requires extrapolation of the linear fit over a long stretch lacking data points in order to determine the x-intercept
  2. Errors in the data obtained at low substrate concentration have a disproportionate impact on the determination of the best linear fit.
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2
Q

How is the Km and Vmax affected by competitive inhibition?

A

Km decreases. Vmax stays the same.

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3
Q

How is the Km and Vmax affected by noncompetitive inhibition?

A

Km stays the same. Vmax decreases.

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4
Q

How is the Km and Vmax affected by uncompetitive inhibition?

A

Km decreases. Vmax decreases.

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5
Q

How is the Km and Vmax affected by mixed inhibition?

A

Km varies. Vmax decreases.

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6
Q

T/F: Prosthetic groups are non-protein organic molecules associated with enzymes?

A

True

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7
Q

T/F: Prosthetic groups are added to proteins after translation?

A

True

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8
Q

T/F: Prosthetic groups are needed in order for some enzymes to catalyze reaction?

A

True

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