Biological molecules - nucleic acids Flashcards

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1
Q

What are pyrimidines?

A

They have single carbon bonds

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2
Q

What bases are pyrimidines?

A

Cytosine and thymine

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3
Q

What are purines?

A

double bonds between the carbon atoms

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4
Q

What bases are purines?

A

Adenine and guanine

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5
Q

What bond is between the phosphates and the sugars?

A

phosphodiester bonds

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6
Q

what is the structure of DNA?

A

a phosphate, pentose deoxyribose sugar and a nitrogenous base

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7
Q

What is the structure of RNA?

A

a phosphate, pentose ribose sugar

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8
Q

What nitrogenous bases are in RNA?

A

Uracil, cytosine, adenine, guanine

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9
Q

How are phosphodiester bonds formed?

A

condensation reaction

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10
Q

what shape does the DNA structure form?

A

a double helix which is anti-parallel

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11
Q

What bond is between the nitrogenous bases?

A

Hydrogen

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12
Q

how are the phosphodiester bonds broken?

A

hydrolysis reactions

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13
Q

what nitrogenous base pairs with adenine?

A

thymine or uracil

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14
Q

what nitrogenous base pairs with guanine?

A

cytosine

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15
Q

What is RNA role?

A

they copy a strand of DNA and remove it out of the nucleus for protein synthesis

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16
Q

How is DNA extracted from plant material?

A

1 - grind sample with pestle and motor to break down cell walls
2 - mix the sample with detergent to break down the cell membrane
3- add salt to break the hydrogen bonds between DNA and water molecules
4 - add protease enzyme to break down proteins associated with the DNA
5 - add a layer of ethanol on top which causes DNA to precipitate out of solution
6 - DNA would appear as white strands between the sample and the alcohol
7- the white layer can be spooled onto a glass rod

17
Q

name 3 different types of RNA found in cells

A

tRNA, mRNA, rRNA