Biological molecules Flashcards
Chemical elements make up carbohydrates
Carbon, hydrogen, oxygen
Chemical elements that make up protein
Carbon, Hydrogen, oxygen and nitrogen
Chemical elements that make up Lipids (fats)
Carbon, oxygen and hydrogen
Carbohydrates, protein and lipids
They are large molecules made up from smaller basic units
Starch and glycogen
simple sugar and glucose molecules
Protein
Amino acids
Lipids
Fatty acids and glycerol
Food test: Glucose
Add Benedict’s Solution into sample solution in test tube.
Heat at 60-70 °c in water for 5 minutes
Take test tube out of water bath and observe the colour
Colour changes from blue to orange or brick red
Food test: starch
We use iodine to test for the presence or absence of starch in a food sample
Add drops of iodine solution to the food sample
Colour change from orange-brown to blue-black
Food test: Protein
Add drops of blutet solution to the food sample
Colour change from blue to violet/purple
Food test: Fat (lipids)
food sample is mixed with 2 cm3 of ethanol and shaken
The ethanol is added to an equal volume of cold water
Cloudy emulsion forming
Metabolism
The sum of all chemical reactions taking place in a cell
Anabolic reactions
Energy and small molecule produces a large molecule
Catabolic reaction
Large molecule produces a smaller molecule and energy
Catalyst
A subatnce that changes the rate of reaction without changing the substance produced
Enzymes
Are biological catalysts which control the rate of reaction that occurs inside the cell and in the whole organisms
Substrate
The molecule that the enzyme acts on is the substrate
Active site
The binding site that holds the substrate in place
How enzymes work (4)
Substrate enter the enzyme’s active site.
Enzyme-substrate complex complex forms and reaction takes place.
Products from and drift away from the enzyme.
The enzyme is free to act on more substrate molecules.
Factors that could affect enzyme activity
Temperature
pH
Enzyme concentration
Substrate concentration
Factors affecting enzymes: temperature (3)
Increasing the temperature increases rate of enzyme reaction.
As the temperature increases kinetic energy of the molecules increases so they collide more often.
Due to this more enzyme/substrate complex forms. Hence more products form in a shorter period of time
Optimum temperature
Temperature at which enzymes works best
Denature (2)
If the temperature and pH changes sufficiently beyond an enzyme’s optimum and the shape of the enzyme irreversibly changes.
This affects the shape of the active site and means that the enzyme will no longer work.
Factors affecting enzymes: pH
The pH inside the cell is neutral and most enzymes are evolved to work best at this pH.