biofilm and diet Flashcards

1
Q

what are the early colonizers

A

streptococcus salivarius, streptococcus mitis, streptococucs oralis

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2
Q

with time gram-negative anaerobes appear what are they

A

prevotella melaninogenica, fusobacterium nucleatum, veillonella spp.

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3
Q

resident microflora prevent overgrowth of pathogenic organisms by

A

-saturation of microbial attachment sites
-effective competition w essential nutrients
-creating unfavorable conditions to invading microbes
-production of inhibitory factors

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4
Q

pellicle formation what time

A

within minutes

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5
Q

attachment of early colonizers what time

A

0-24hrs

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6
Q

microcolony formation by co-adhesion and growth

A

4-24hrs

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7
Q

species diversity through microbial succession

A

1-7 days

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8
Q

mature biofilm

A

1 week or older

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9
Q

what is the bridging organism between early and late colonizers

A

F. nucleatum

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10
Q

predominance by what gram is in 5 days

A

gram -

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11
Q

rapid colonization by pioneer species are what

A

gram + cocci and rods

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12
Q

biofilm composed of microcolonies (how much volume) distributed in shaped matrix (how much volume)

A

microcolonies (15-20% volume)
shaped matrix (75-80% volume)

has voids and water channels also

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13
Q

what is the backbone of biofilm

A

exopolysaccharides (EPS)

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14
Q

normal microflora of fissures is sparse or dense, and favored metabolism is derived from what

A

sparse
sugar breakdown (saccharolytic metabolism)

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15
Q

what dominates in fissures

A

streptococci, w few gram - or anaerobic organisms

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16
Q

where is more diverse microflora and there is a lot of what there

A

gingival crevice
many gram - anaerobic and proteolytic species

17
Q

what is the intermediate combination of microflora of fissures and gingival revices

A

approximal surfaces

18
Q

streptococcus mutans are what type of bacteria

A

facultative anaerobic

19
Q

what are the species associated w sever caries

A

s mutans
actinomyces gerencseriae
bifidobacterium spp
s salivarius
lactobacillus fermentum
veioolonella spp

20
Q

what are 3 cariogenic features of bacteria

A

-ability to transport fermentable sugars PEP-PTS system
-acidogenic and aciduric
-produce extracellular polysaccharides (EPSs) - glucans and fructans

21
Q

who noted sugar was detrimental to teeth and gingiva

A

Pierre fauchard

22
Q

what are the monosaccharides and disaccharides

A

mono - glucose, fructose, galactose
di - sucrose, maltose, lactose

23
Q

caloric bulk sweetener sorbitol

A

6 carbon sugar alcohol that cannot be utilized by microorganisms that dominate dental plaque

but strains of mutans streptococci, lactobacilli, and other oral microorganisms do ferment sorbitol

24
Q

caloric bulk sweetener xylitol

A

5 carbon sugar, cannot be fermented by most oral streptococci
exterts bacteriostatic effect on mutans streptococci (creates cell degradation, intracellular vacuoles, and other damages to cell)

25
Q

PEP-PTS does what

A

sugar (glucose) into membrane of bacteria

26
Q

pyruvate yields

A

lactic acid
accetic acid
ethanol
formaic acid
(decreases pH in biofilm)

27
Q

on cell membrane, have glucosyltransferase and fructosyltransferase which produces what molecules and what for the biofilm

A

produce glucan and fructan
(produces sticky matrix in the biofilm out of sucrose)

28
Q

villanelles species what special about it

A

do not use glucose or sugar, uses lactic acid from other bacteria, to make acetic acid, formic acid, propionic acid (which lowers pH)

29
Q

can bacteria use xylitol?

A

bacteria use PEP-PRS system for xylitol to bring it in the cell membrane, but cannot be used by bacteria so it spits out and cannot create ATP

xylitol creates vesicles, and vacluoes that promotes destruction of the cell/bacteria