Bioenergetics [ETC + summary]

Question 1

Etc inputs

Answer 1

10NADH
2FADH2
6O2

Question 2

ETC outputs

Answer 2

10NAD+
2FAD
12H2O

Question 3

Where does ETC occur?

Answer 3

Inner membrane

Question 4

Complex 1 
Name:
Description:
H+ transferred:
Pump?:

Answer 4

Complex 1 
Name: NADH oxidase
Description: converts NADH —> NAD+. FMN sends e- flying down a wire of Fe-S, out and into Q.
H+ transferred: 4 (+ 2 at end?)
Pump?: Yes

Question 5

Complex 2 
Name:
Description:
H+: 
Pump?:

Answer 5

Complex 2 
Name: Succinate Q reductase
Description: FADH2 —> FAD. electrons sent to Q.
H+: none
Pump?: no

Question 6

Complex 3
Name: 
Description: 
H+: 
Pump?:

Answer 6

Complex 3
Name: 3 Cyt b-C1
Description: Electrons come in from Q, to Cyt-b —> Fe-S —> Cyt-C. Then sent to cyt-C carrier.
H+: 4
Pump?: No

Question 7

Complex 4 
Name: 
Description:
H+:
Pump?:

Answer 7

Complex 4 
Name: 4 Cyt-C oxidase. 
Description: electrons from cyt-c carrier. Cyta —> Cyta3. The 2H transferred + 0.5O2 are used to make water.
H+: 2
Pump?: yes

Question 8

How many more electrons do NADH move than FADH2.

Answer 8

4

Question 9

Name the two types of super complexes and how many protons they transfer

Answer 9

Complex 1,3,4
- 10 H

Complex 2,3,4
- 6 H

Question 10

What poisons affect complex 1?

Answer 10

Rotenone and anaesthetics

Question 11

What poisons affect complex 3

Answer 11

Antimycin (blocks Q cycle)

Question 12

What poisons affect complex 4

Answer 12

Cyanide / O2 (binds to Fe heme)

Question 13

Name the 4 parts of the ATP synthase

Answer 13

Strator
Rotor
Internal rod
Catalytic knob

Question 14

Path of H+ in ATP synthase

Answer 14

Strator
One turn in rotor
2nd strator
Matrix

Question 15

How does the internal rod interact with the catalytic knob?

Answer 15

Rod spins inside rod, pushing subunits in the knob as it stays still. The spinning activates sites in the knob which makes ATP. Rod is not straight.

Question 16

Features of the malate shuttle to bring FADH2 and NADH into the ETC. ATP? Speed? Complicated or not? How efficient?

Answer 16

2.5ATP
slow
Complicated
Efficient

Question 17

Features of the G3PDH shuttle to bring FADH2 and NADH into the ETC. ATP? Speed? Complicated or not? How efficient?

Answer 17

1.5ATP
Less efficient due to FADH2 use
Fast

Question 18

Cause of Leigh’s disease

Answer 18

Mutation in C ring of ATP synthase

Question 19

Symptoms of Leigh’s disease

Answer 19

?

Question 20

Describe Cyt-C structure and how it transfers electrons

Answer 20

It binds to an electron becoming reduced, then oxidizes and changes colour. It’s absorbance changes.