biochem: lab techniques Flashcards

1
Q

blot mnemonic

A

SNoW DRoP: Southern: DNA, Northern: RNA, Western: Protein. southwestern blot identifies DNA-binding proteins

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2
Q

cre-lox system

A

inducibly manipulate genes at specific developmental points (e.g. to study a gene whose deletion causes embryonic death)

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3
Q

RNA interference

A

fancy technique where dsRNA is complementary to target mRNA, causing degradation and decreased gene expression

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