BIO101 LAB 1-6 Flashcards
Ton
one million 1,000,000
How many meters are in 1 decimeter?
0.1m
How many grams are in 1 kilogram?
1000g
How many grams are in 1 microgram?
0.000001g
deci
one-tenth 0.1
How many meters are in 1 milimeter?
0.001m
sum of all measures/ # of measures
average
How many meters are in 1 kilometer?
1000m
highest result value- lowest result value
range 3
kilo
one thousand 1,000
the group that nothing has been changed to
control
centi
one hundredth
H+ = 1 x 10 ^-7 what is the pH?
7 *ph is equal to exponent
What is the equation to convert from one unit to another?
given unit x
desired unit/given unit
ex: 20m to millimeters
20m x 1000mm/1m = 20,000mm
How many grams are in 1 nanogram?
0.000000001g
How many grams are in 1 decigram?
0.1g
mili
one thousandth 0.001
the thing affected by the change you made (*must be measurable)
dependent variable
what is pH?
a measurement of the relative balance of the ionized forms of water
micro
one millionth 0.000001
How many meters are in 1 centimeter?
0.01m
nano
one billionth 0.000000001
factor that is deliberately changed
independent varaible
How many meters are in 1 nanometer?
0.000000001m
releases hydogen ions
acids
the group(s) where the IV is deliberatel altered by the experimenter
experimental group
How many grams are in 1 centigram?
0.01g
substances that release hydroxide ions (OH-)
bases
factors kept consistent between the groups
constants
How many grams are in 1 miligram?
0.001g
How many meters are in 1 micometer?
0.000001m
What influences enzyme activity?
pH, temperature, etc
type of microscope that uses a series of lenses and a light source
compound light miscroscope
type of microscope that is used to view objects in 3 dimensions
steroscopic microscope
type of microscope that filters out certain light rays to view certain structures better (especially clear objects)
phase-contact microscope
type of microscope that shines a beam of electrons across the surface of an object in a vacuum that gives an image of surface and dimensions of an object
scanning electron microscope
type of microscope that uses electrons in a vacuum to view ultra-thinly sliced objects that have been treated with heavy metal salts to imporce contrast
transmission electron microscope
which knob would fine tune the image of the specimen?
fine focus
how many objective lenses are there?
3 or 4
what do we call the shortest lens?
scanning power
what is the power of the middle lens?
10x
what is the power of the “high dry” lens?
40x
what do we need to add to see the highest magnification?
immersion oil
eye piece
ocular lens
what are the series of 4 lenses
4x, 10x, 40x, and 100x with oil immersion
lever or dial under the stage that regulates the amount of light going through the specimen
iris diaphgram
a series of lenses that you can move up and down to condense and clarify the light shinning through the object
condenser
large know found on both sides of the microscope the moves stage and is ONLY used with 4x lense
coarse adjustmend knob
How do you find the total magnification?
multiply objective lens by 10x (ocular lens)
used for living of recently living tissues
wet mounts
contain non-living tissue (dead)
prepared slides
major groups to classify organisms
hint:
Dear King Phillip Came Over From Great Spain
Domain, Kingdom, Phylum, Class, Order, Family, Genus, and Species
the circle that you see when you look through the ocular lens
field of view
the space between the slide and the objective
working distance
the capactiy to distinguis between two adjacent points
resolving power
Is Prokarya a domain of life?
NO
What are the 3 domains of Life?
Archaea, Bacteria, and Eukarya
cell membranes are selectively permeable, meaning that…
some things can enter/exit them
forms a thin, protective layer around the cell
cell membrane
this type of transport requires energy from the cell
active transport
this type of transport doesn’t require energy expenditure from the cell
passive transport
includes simple diffusion, facilitated diffusion, osmosis, and filtration
passive movement
requires ATP and the cell to expand energy, endocytosis, and exocytosis
Active movement
the movement of particles from an area of high concentration to an area of low concentration until they are distributed evenly
diffusion
the difference in concentration areas is called the
concentration gradiant
this occurs when certain molecules can only pass through the cell membrane with assistance from other cellular structures such as large proteins
facilitated diffusion
the diffusion of water molecules through a semipermeable membrane from a region of higher water concentration (lower solute concentration) to a region of lower water concentration (higher solute concentration)
osmosis
the process in which the plasma membrane engulfs extracellular material, forming membrane-bound sacs that enter the cytoplasm and thereby move material into the cell
endocytosis
“cell drinking” the cell takes in fluid filled droplets by forming a pocket in the cell membrane and then pinching off with the fluid and any small nutrients, amino acids, glucose, vitamins, etc. inside
pinocytosis
“cell eating” the cell engulfs a particle by sending out pseudopods and then consumes the particle with enzymes.
phagocytosis
the process in which intracellular material is enclosed within a membrane-bound sac that moves to the plasma membrane and fuses with it, releasing the material outside the cell
exocytosis
particles in the solvent
solute
liquid the solute is in
solvent
the mixture
solution
Iso-
equal
Tonic
tension, refers to amount of solute in the solution
same amount of solute particles in the cell as in the solution; a cell neither gains now loses water
isotonic solution
higher solute concentration outside the cell than inside the cell; a cell loses water and the cytoplasm shrinks
hypertonic solution
higher solute concentration inside the cell than outside the cell; a call gains water
hypotonic solution
differences between animal cell and plant cell
animal cells=round plant cells= rectangular animal cells= no cell wall plant cells= cell wall plants= autotrophs animals=heterotrophs
similarities between animal cell and plant cell
- eukaryotic
- membrane-bound organelles
mostly unicellular eukaryotes mostly found in water
protists
protists consist of a group that has a common ancestor
polyphyletic group
The 3 modes of nutrition for protists are…
photoautotrophs, heterotrophs, and mixotrophs
hair-like structures that cover a cellular surface of a protist, and move the protist with coordinated back and forth beating-like motions
cilia
these are tail-like appendages that whip through the water, propelling the protist forward. There can be one, two, or several of these on the surface of a protozoan
flagella
this is the movement of cytoplasm within. cells, and typically serves to circulate nutrients and move organelles throughout the cell
cytoplasmic streaming
various lineages of protists
supergroups
this supergroup of protists includes parasites as well as many predatory and photosynthetic species
supergroup excavata
this supergroup of protists includes some of the most important photosynthetic organisms on Earth as well as predatory and parasitic species
may not all have the same most recent common ancestor
supergroup chromalveolata
this supergroup of protists contains amoebas with threadlike pseudopodia. pseudopodia are extensions of the cytoplasm formed by cytoplasmic streaming
supergroup rhizarians
this supergroup of protists contains the red algae, green algae, and Kingdom Plantae
supergroup archaeplastida
this supergroup of protists contains the amoebas, slime molds, as well as the Fungi and Animal kingdoms
supergroup unikonta
single-celled, prokaryotic organisms that are about 10-100 times smaller than the average human cell
Domain Bacteria
this strain of bacteria contains 1 thick peptidoglycan layer and 1 plasma membrane. This strain appears violet
Gram ‘Positive’
this strain of bacteria contains an inner and outer plasma membrane with a thinner peptidoglycan layer in-between. This strain appears red/pinkish
Gram ‘Negative’
this is a material made of the linking of various proteins that form a very strong chain fence-like structure that is also permeable to most materials
peptidoglycan
Only difference between gram positive and gram negative
thick layer peptidoglycan/ one cell membrane= positive
thin layer/ 2 cell membrane =negative
one cell membrance=
(purple of blue) refers to organisms that retain the iodine complex
gram positive
(pink or red) refers to organisms that lose the iodine complex
gram negative
Bacteria reproduce by…
pili and binary fission
what helps dry the bacteria during gram straining?
heating it
What is the order of chemicals used in gram straining?
crystal violet, grams iodine, 95% alcohol, and safranin