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BIO CHEM 4 Flashcards

1
Q

cDNA, a term used in recombinant DNA technology means

Competitive DNA
Chemical DNA
Complex DNA
Complementary DNA

A

Complementary DNA

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2
Q

The process of introduction of foreign DNA into an animal cell is called

Transversion
Conversion
Inversion
Transfection

A

Transfection

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3
Q

A segment of DNA that reads from the same forward and backward is called

Palindromic DNA
Complementary DNA
Plasmid DNA
Copy DNA

A

Palindromic DNA

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4
Q

Which of the following enzyme is used in PCR?

Taq DNA polymerase
HRP
EcoRI
EcoRII

A

Taq DNA polymerase

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5
Q

Which of the following is helpful in distinguishing DNA of one individual from another?

PCR
Reverse transcriptase
cDNA
RFLP

A

RFLP

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6
Q

Which of the following is the correct order of organization of genetic material from largest to smallest?

Genome, chromosome, gene, nucleotide
Nucleotide, gene, chromosome, genome
Gene, nucleotide, chromosome, genome
Chromosome, genome, nucleotide, gene

A

Genome, chromosome, gene, nucleotide

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7
Q

Which of the following codons is not a termination codon for protein synthesis?

UUU
UAG
UAA
UGA

A

UUU

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8
Q

Which of the following are the two methods of screening?

Hybridization and PCR
ELISA and blotting
ELISA and PCR
PCR and RFLP

A

Hybridization and PCR

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9
Q

Short sub-sequence of a cDNA sequence is

Expressed sequence tag
Sequence tagged site
cContig
YAC

A

Expressed sequence tag

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10
Q

Short DNA sequence having single occurrence in genome is

Expressed sequence tag
Sequence tagged site
Contig
YAC

A

Sequence tagged site

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11
Q

A set of overlapping DNA segments that together represent a consensus region of DNA is

Expressed sequence tag
Sequence tagged site
Contig
YAC

A

Contig

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12
Q

Which of the following statements is false?

BAC is a circular DNA molecule
YAC is a linear DNA molecule
Taql is a DNA polymerase
BAC is a linear DNA molecule

A

BAC is a linear DNA molecule

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13
Q

Which of the following is not denatured by heating steps during PCR?

DNA pol
RNA pol
Restriction enzymes
DNA ligase

A

DNA pol

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14
Q

Which of the following statements regarding DNA fingerprinting is false?

DNA fingerprinting cannot be used for paternity testing

DNA profile using STR (short terminal repeats) is unique to an individual

PCR is used for DNA profiling

Forensic analysis makes use of SNPs (single nucleotide polymorphisms) in coding sequences to distinguish between individuals

A

DNA fingerprinting cannot be used for paternity testing

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15
Q

How are expression vectors useful?

To produce protein products
Used for genomic libraries
Used for chromosome synthesis
Used for finger printing

A

To produce protein products

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16
Q

Who was the first person to develop DNA finger printing?

David Suzuki
Khorana
Alec Jaffreys
Gilbert

A

Alec Jaffreys

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17
Q

Southern blotting technique is used in

Monoclonal antibody production
In vitro culture
Genetic finger printing
Polymerase chain reaction

A

Genetic finger printing

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18
Q

Electrophoresis helps to separate

DNA segments
Cells from DNA
Tissues
RNA from DNA

A

DNA segments

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19
Q

During DNA profiling, DNA nucleotides hybridized with probe can be detected through

Electrophoresis
Polymerase chain reaction
Autoradiography
Hybridoma

A

Hybridoma

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20
Q

Which of the following term is associated with DNA finger printing?

Hybridoma
Site specific mutagenesis
Shotgun cloning
RFLP

A

RFLP

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21
Q

Recombinant DNA construction involves

Cleaving DNA with restriction endonuclease and joining with ligase

Cleaving DNA with ligase and joining with endonuclease

Cleaving and joining DNA with restriction endonuclease

Cleaving DNA with restriction endonuclease and joining with polymerase

A

Cleaving DNA with ligase and joining with endonuclease

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22
Q

The study of full complement of proteins expressed by a genome is called

Proteome
Proteomics
Genomics
Protein formation

A

Proteomics

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23
Q

The effects of protein on an entire organism is described in

Phenotypic function
Cellular function
Molecular function
Structural genomics

A

Phenotypic function

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24
Q

The network of interactions engaged in by protein at cellular level is described in

Phenotypic function
Cellular function
Molecular function
Structural genomics

A

Cellular function

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25
Q

The precise biochemical activity of a protein is described in

Phenotypic function
Cellular function
Molecular function
Structural genomics

A

Molecular function

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26
Q

Sequencing of genomic DNA is included in

Phenotypic function
Cellular function
Molecular function
Structural genomics

A

Structural genomics

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27
Q

The goal of structural proteomics project is

To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function

To identify and sequence of all the genes present in the human body

To introduce new genes to human beings

To remove disease causing genes from humans

A

To crystallize and determine the structure of as many proteins as possible, in many cases with little or no existing information about protein function

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28
Q

Conserved gene order can be termed as

Ortholog
Synteny
Paralog
Microarray

A

Synteny

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29
Q

Genes of different species but possessing a clear sequence and functional relationship to each other are

Ortholog
Synteny
Paralog
Microarray

A

Ortholog

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30
Q

Genes of same species, similarly related to each other are

Ortholog
Synteny
Paralog
Microarray

A

Paralog

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31
Q

Collection of microscopic DNA spots attached to solid surface are

Ortholog
Synteny
Paralog
Microarray

A

Microarray

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32
Q

Rauolfia serpentine, to save this plant under the threat of extinction, which of the following techniques is useful?

Genetic engineering
In vitro culture
DNA fingerprinting
Hybridoma technology

A

In vitro culture

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33
Q

Transgenic organisms are generally

Extinct organisms
Naturally occurring and endemic
Produced by traditional plant breeding technique
Produced by gene transfer technology

A

Produced by gene transfer technology

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34
Q

Dolly, the first animal produced by cloning is a

Cow
Sheep
Rat
Dog

A

Sheep

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35
Q

Gene therapy is a technique preferred to cure inherited diseases by

Repairing the faulty gene
Introducing the correct copy of the gene
Adding new cells to the body
PCR

A

Introducing the correct copy of the gene

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36
Q

Glucose is added to the tissue culture media as a/an

Carbon source
Growth regulator
Solidifying agent
Antibiotic

A

Carbon source

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37
Q

Explant can be a

Cut part of the plant used in tissue culture
Plant extract used in tissue culture
Source of growth regulators added to media
Solidifying agent

A

Cut part of the plant used in tissue culture

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38
Q

Meristem culture helps in developing which of the following?

Tall plants
Disease resistant plants
Virus free plants
Hybrid plants

A

Virus free plants

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39
Q

Which of the following is used to transfer genes in plants?

Ti plasmid
pBR 322
EcorI
pUC 18

A

Ti plasmid

40
Q

Which of the following bacterium is used for gene transfer in plants?

Agrobacterium
Azatobacter
Rhizobium
Ecoli

A

Agrobacterium

41
Q

Production of a large number of genetically similar plants through plant tissue culture is called

Recombinant DNA technology
Gene therapy
Micro propagation
Hybridoma technology

A

Micro propagation

42
Q

Which of the following is an essential fatty acid?

Linolenic
Palmitic
Oleic
Stearic

43
Q

Which of the following is a polar derivative of cholesterol?

Bile salt
Oestrogen
Vitamin D
Progesterone

44
Q

Which of the following fatty acid has the least melting point?

Palmitic acid
Stearic acid
Arachidonic acid
Timnodonic acid

A

Timnodonic acid

45
Q

Out of the following which is not a source of glycerol?

Adipolysis
Glycolysis
Glycogenolysis
Diet

A

Glycogenolysis

46
Q

Which of the following is false about fatty acids?

Melting point of fatty acids decreases with increase in degree of saturation

Lipids in tissues that are subjected to cooling are more unsaturated

Naturally occurring unsaturated long-chain fatty acids are nearly Trans-configuration

The membrane lipids contain mostly unsaturated fatty acids

A

Naturally occurring unsaturated long-chain fatty acids are nearly Trans-configuration

47
Q

Which of the following fatty acid has 16 carbon atoms?

Linolenic acid
Oleic acid
Palmitic acid
Stearic acid

A

Palmitic acid

48
Q

Which of the following is a hydroxyl fatty acid?

Linoleic acid
Palmitic acid
Linolenic acid
Cerebronic acid

A

Cerebronic acid

49
Q

Out of the following, which is not an essential amino acid?

Linolic acid
Linolenic acid
Arachidic acid
Arachidonic acid

A

Arachidic acid

50
Q

The number of milligrams of KOH required to neutralize the free and combined fatty acid in one gram of a given fat is called

Saponification number
Iodine number
Acid number
Polenske number

A

Saponification number

51
Q

Which of the following is a storage form of lipid?

Glycolipid
Phospholipid
Sufolipid
Triacyl glycerol

A

Triacyl glycerol

52
Q

Out of the following, cholesterol does not serve as a precursor for which compounds?

Vitamin D
Sex hormones
Bile salts
Bile pigments

A

Bile pigments

53
Q

Which of the following is a sphingophospholipid?

Lecithin
Sphingomyelin
Plasmolegen
Cardiolipin

A

Sphingomyelin

54
Q

Which of the following glycerophospholid acts as a lipotropic agent?

Cardiolipin
Phosphatidylserine
Phosphatidylinositol
Phosphatidylcholine

A

Phosphatidylcholine

55
Q

Which of the following phospholipids is a component of inner mitochondrial membrane?

Plasmologen
Cephalin
Lecithin
Cardiolipin

A

Cardiolipin

56
Q

In which of the following glycerophospholipids two phosphatidic acids share a single glycerol?

Cardiolipin
Phosphatidylserine
Phosphatidylinositol
Phosphatidylcholine

A

Cardiolipin

57
Q

Platelet activating factor stimulates the release of which of the following compounds?

Vasopressin
Serotonin
Adrenaline
Cortisol

58
Q

Which of the following group of membrane lipids predominate in plant cells?

Galactolipids
Sphingolipids
Glycerophospholipids
Archaebacterial ether lipids

A

Galactolipids

59
Q

Which of the following membrane lipids have a direct glycosidic linkage between the head-group sugar and the backbone glycerol?

Glycolipids
Phospholipids
Sphingolipids
Ether lipids

A

Glycolipids

60
Q

The backbone of phospholipids is

L-glycerol 1-phosphate
L-glycerol 3-phosphate
D-glycerol 3-phosphate
sn-glycerol 1-phosphate

A

L-glycerol 3-phosphate

61
Q

What is the head-group alcohol in plasmolegen and platelet-activating factor?

Alkene
Choline
Alkane
Acetic acid

62
Q

Which of the following does not contain glycerol?

Glycerophospholipids
Galactolipids
Sphingolipids
Triacylglycerols

A

Sphingolipids

63
Q

Phosphorylation of phosphatidylinositol yields

Phosphatidylinositol 4, 5-biphosphate
Phosphatidylinositol 3, 5-biphosphate
Phosphatidylinositol 3, 4-biphosphate
Phosphatidylinositol 5, 6-biphosphate

A

Phosphatidylinositol 4, 5-biphosphate

64
Q

Which of the following vitamin is derived from cholesterol?

A
B
C
D

65
Q

What leads to the activation of protein kinase C?

Release of intracellular Ca2+ + diacylglycerol
Release of intracellular Mg+ + diacylglycerol
Release of intracellular Ca2+ + glycerol
Release of intracellular Ca2+ + triacylglycerol

A

Release of intracellular Ca2+ + diacylglycerol

66
Q

Which of the following serves as a specific binding site for the proteins involved in membrane fusion during exocytosis?

Phosphatidylinositol
Phosphatidylinositol 4, 5-biphosphate
Phosphatidylinositol 3, 5-biphosphate
Phosphatidylinositol 3, 4-biphosphate

A

Phosphatidylinositol 4, 5-biphosphate

67
Q

Which of the following hormone is responsible for the activation of phospholipase C?

Serotonin
Cortisol
Vasopressin
Adrenaline

A

Vasopressin

68
Q

An example of glycerophospholipid involved in cell signaling is

Cardiolipin
Phosphatidic acid
Phosphatidylcholine
Phosphatidylinositol

A

Phosphatidylinositol

69
Q

Which of the following type structure contains all the three glycosphingolipids?

B structure
A structure
O structure
AB structure

A

O structure

70
Q

The lipids with potent bio activities derived from isoprenoid precursors are common in

Vitamin A, K, ubiquinone and dolichol
Vitamin A, D, ubiquinone and dolichol
Vitamin A, B, D and K
Vitamin A, B, K and dolichol

A

Vitamin A, K, ubiquinone and dolichol

71
Q

Which of the following vitamin is responsible for Ca2+ and phosphate metabolism?

A
K
E
D

72
Q

How many products are obtained by the hydrolysis of phosphatidylinositol by phospholipase C?

1
2
3
4

73
Q

Which of the following is false about lipids?

They are either strongly hydrophobic or amphipathic
They are more soluble in water
Extraction of lipids from tissues require organic solvents
They are insoluble in water

A

They are more soluble in water

74
Q

Which would move faster in thin layer chromatography?

Beeswax
Phosphatidylinositol
Cholesterol
Steroid

75
Q

In which type of chromatography, solvents of increasing polarity are passed through a column of silica gel?

High performance liquid chromatography
Thin layer chromatography
Adsorption chromatography
Gas-liquid chromatography

A

Adsorption chromatography

76
Q

Phosphatidylinositol, phosphatidylglycerol and phosphatidylserine are easily separated by

Absorption chromatography
TLC
HPLC
Gas-liquid chromatography

77
Q

For the determination of fatty acid composition, transesterification is done in a warm aqueous solution of

KCl+methanol
KOH+methanol
NaOH+methanol
H2O+methanol

A

NaOH+methanol

78
Q

A mixture of fatty acyl methyl esters are separated based on

Charge
Chain length and degree of saturation
Molecular weight
Ionic size

A

Chain length and degree of saturation

79
Q

Which technique is preferred in the separation of fatty acyl methyl esters from a mixture?

Gas-liquid chromatography
Absorption chromatography
TLC
Centrifugation

A

Gas-liquid chromatography

80
Q

The dye used in TLC for detecting separated lipids by spraying the plate is

Mordant
Alizarin
Rhodamine
Fuchsin

81
Q

Which of the following is not a phospholipase?

A
C
D
K

82
Q

In which type of chromatography lipids are carried up a silica gel coated pate by a rising solvent front, less polar travels farther than the more polar ones?

Absorption chromatography
Thin layer chromatography
Gas-liquid chromatography
HPLC

A

Thin layer chromatography

83
Q

Which of the following acts as a passive electrical insulator?

Myelin sheath
Plasma membrane
Inner mitochondrial membrane
Outer mitochondrial membrane

A

Myelin sheath

84
Q

Which of the following membranes contain low cholesterol and high cardiolipin?

Myelin sheath
Plasma membrane
Inner mitochondrial membrane of hepatocyte
Cell wall

A

Inner mitochondrial membrane of hepatocyte

85
Q

Which of the following is important in signal transduction triggered by hormones?

Phosphatidylinositol and its derivatives
Phosphatidylserine and its derivatives
Phosphatidylglycerol and its derivatives
Phosphatidylethanolamine

A

Phosphatidylinositol and its derivatives

86
Q

Which of the following are the major component of chloroplast membrane of plants and are absent from animal cells?

Glycolipids
Phospholipids
Ether lipids
Triacylglycerols

A

Glycolipids

87
Q

How many types of lipid aggregates are formed when amphipathic lipids are mixed with water?

1
2
3
4

88
Q

Which of the following types of lipid aggregates has the maximum stability?

Micelles
Bilayer
Liposome
Myelin sheath

89
Q

When cross sectional area of the head group and acyl side chain is similar, which of the following lipid aggregates formation is favored?

Micelles
Bilayer
Liposome
Myelin sheath

90
Q

When cross sectional area of the head group is greater than that of the acyl side chain, which of the following lipid aggregates formation is favored?

Micelles
Bilayer
Liposome
Myelin sheath

91
Q

Which of the following is a choline containing lipid?

Sphingomyelin
Phosphatidylserine
Phosphatidylglycerol
Phosphatidylethanolamine

A

Sphingomyelin

92
Q

A platelet is able to play its role in the formation of blood clot when one of the glycerophospholipids in the plasma membrane moves into the outer leaflet

Phosphatidylserine
Phosphatidylethanolamine
Phosphatidylinositol
Phosphatidylglycerol

A

Phosphatidylserine

93
Q

Which of the following glycerophospholipids is not common in inner leaflet (cytoplasmic)?

Phosphatidylserine
Phosphatidylethanolamine
Phosphatidylinositol
Phosphatidylcholine

A

Phosphatidylcholine

94
Q

Which of the following has high lipid content?

Myelin sheath
Plasma membrane
Inner mitochondrial membrane
Outer mitochondrial membrane

A

Myelin sheath

95
Q

Plasma membrane is permeable to

ATP
Glucose
K+
Urea

96
Q

How are the intrinsic proteins in cell membrane distributed?

Uniform
Random
Symmetric
Asymmetric

A

Asymmetric

97
Q

END

BIO CHEM (10 decks)

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