Bio 3 - I (continued notes from the IRL flashcards)
gene expression
The process in which gene are transcribed into RNA molecules, which is then transformed into mRNA and translated into proteins(amino acids form)l. Essentially genotype to phenotype
codons
When translating the mRNA into proteins, the ribosomes in the cytoplasm read the bases (adenine, thymine, guanine, uracil) in the form of codons (three bases). For example, AGU, ACU, this is how it is read. There are a total of 64 codons for a total of 20 amino acids, meaning different codons may represent the same amino acid.
Start codons vs. end codon
Some codons in the mRNA don’t code for anything, but instead tell when the ribosome should start translating and end translating. The start codon is AUG, and the end codons is UAA, UGA, or UAG
What is the genetic code?
It tells the ribosome which codon corresponds to what amino acid
After translation, how is the protein built?
tRNA takes the correct amino acids using the genetic code, and builds the new protein
What are the three steps for translation?
Initiation, elongation, and termination
Initiation
In this process, this is when the ribosome finds the start codon(AUG) and then binds to the mRNA, and then the tRNA puts the anticodon for the start codon along with the amino acid (methionine), and then the large protein comes and attaches to the small protein
tRNA
a group of complementary nucleotide that also have attached an amino acids that is appropriate for the pairing.
What happens during elongation?
This is when a covalent bond is formed when the second tRNA nucleotide is attached to the first, and the amino acids make a covalent bond, in which they share electron(s).
What is ribosomes role throughout this process?
Ribosome makes sure that the tRNA and the mRNA are properly and appropriately binded, and continue to move right in order to bond the complete protein made up of amino acids
What are proteins made up of?
Amino acids
What happens when the ribosome reaches the stop codon?
This is UAA, UGA, or UAG, and because of this, the ribosome gets the release factor protein in order to release the bonds between the mRNA and the tRNA, maintaining the bonds for the polypeptide.
Release factor protein
Used to stop the sequence once hit the stop codon
rRNA
component of ribosomes(this is all you need to know just memorize this)
Translation Errors
This can be a removable, substitution, or addition mutation (of a base of DNA). Because of this, the transcription process could mess up the mRNA, and when doing tRNA with each nucleotide, it can lead to a completely new protein(because of one nucleotide mess up)