basic Flashcards
1
Q
which keratin de novo in stratum basale
A
k5/14
2
Q
Which keratins are on acidic chr 17?
A
K10-20, 9-28
3
Q
Which layer of skin has desmosomal connections calcium dependent
A
stratum spinosum spines
4
Q
Il23 is released from?
A
keratinocytes
5
Q
IL1,6,8,TNF-a, is released from?
A
keratinocytes
6
Q
Mucocutaneous receptors found on glans penis, clitoris, labia minora, peri-anal area, and vermillion border of the lips
A
krause
7
Q
mononuclear phagocytic cells staining
A
CD6
8
Q
% dry wt elastin
A
4 %
9
Q
melanocyte:keratinocyte
A
1:36
10
Q
keratinized epidermis
A
24 w
11
Q
type 1 ? type 3 collagen
A
>
12
Q
What fibres have less elastin and more fibrillin; run parallel in thin bands within the
reticular dermis
A
elaunin fibres
13
Q
Elastic fibers: ?% elastin, wrapped by fibrillin microfibrils (mutated in Marfan’s)
A
90%elastin
14
Q
typical amino acids found in elastic fibers, and crosslinkfibrillin requires lysyl oxidase (copper-dependent process)
A
desmosine isoesmosine
15
Q
contain no elastin; run perpendicular from DEJ within superficial papillary dermis
A
Oxytalan fibers:
16
Q
laminin 5 is found in which part of BMZ?
A
lamina densa,
17
Q
amnion contains what type collagen ?
A
type 7 collagen ,
18
Q
granulation tissue type collagen?
A
colagen 3,
19
Q
proline hydroxyproline constituents of
A
collagen
20
Q
function odland bodies ?
A
SC h20 loss barrier
21
Q
basal–SC transition time
A
14d
22
Q
decreased lamellar granules occurs in ?
A
flegels ,
23
Q
absent lamellar granules
A
harlequin icthyosis
24
Q
warts renal transplant
A
k13
25
abrupt cessation mitotic activity rapidly dividing hair matrix
anagen effluvium
26
EB +MA ,
Plectin,
27
JEB +PA
integrin,
28
Ocular CP-JEB PA
B4 of a6b4 integrin
29
EBS weber cockayne
k5
30
mucosal melanoma
KIT
31
In upper spinous layer and throughout the stratum granulosum
| – 75% of CE’s mass (major protein component of CE)
loricrin
32
more dispersed melanosomes
darker skin
33
wound healing , granulation tissue
fibronectin
34
macrophage
wound healing,
35
BM collagen
collagen 4,
36
,melanosomes after
golgi apparatus
37
• nail keratin
k6b/17,
38
cutis laxa marfans anetoderma, pXE
elastic fibres fragmentation-
39
kf insert into
desmosomes,
40
larger singly dispersed melanosomesbut same melanocyte no,
dark skin
41
Tyrosine->DOPA
> DOPAquinone,
42
WB body is a
protein
43
BP 230-desmoplakin belongs to what family
plakin
44
melanocyte genesis occurs when
3rd month
45
k6-16 is upregulated in
psoriaiss
46
human sebum is differntiated from other lipid by
wax esters
47
mononuclear phagocytes express
CD11c, CD6; CR4
48
PPARI3 is expressed in
LPP
49
CD 34 + stainin
mast cell
50
integrins intermediate filaments insert into
+ECM
51
guinea pig esophagus,
folgo selvagem
52
black fly
folgo selvagem
53
no value in neonatal LE,
DIF
54
zeis
specialised sebaceous (eyelash
55
montgomery tubercle
areolar sebaceous)
56
meibomian
eyeld sebaceous
57
plakoglobin family
armadillo
58
postganglionic
acetylcholine
59
CPA cytarabine cipro cephalexin common point
eccrine []
60
apocrine not present in
palms
61
sebaceous ca stain
EMA
62
NOT under neural ctrl,
sebaceous
63
abn collagen xlink
homocystinuria
64
hair follicle direction growth
cephalad to caudal
65
urocanic acid
UVA filter
66
cs receptor location
cytoplasm
67
PNP /senear usher DIF
BMZ intercellular
68
LP DIF
granular DEJ,
69
NC16a BP180, ,
BP
70
SCLE DIF
granular cytoplasm nucleus
71
primodrial hair embrogeneisis
9 w,
72
periderm
vernix caseosa
73
•DHT MSH prolactin, ACTH, MMP zn,
hair follicle
74
Inside to outside
Cuticle - IRS - Huxley-Henle - ORS - glassy/vitreous
75
Three compartments, stains red because citrulline
Inner root sheath: Three compartments, stains red because citrulline
1. ) Henle’s layer - keratinizes first
2. ) Huxley’s layer
3. ) Cuticle
76
critical line auber-
widest diameter,
77
first area where change from vellus to terminal hair occurs in puberty
pubic region. Axillary hair 2 years later
78
change of content of Odland bodies
As they move up in granular cell layer content changes neutral sugars lipids, and/or proteins, hydrolytic enzymes, and free sterols phospholipids diminish, neutral lipids and sphingolipids increase.
Cholesterol sulfate increases spinous to the granular layer b decreases stratum corneum
79
Osteoclastic
giant cells with eosinophilic cytoplasm
80
___separates the trilaminar plasma membrane from the lamina densa
lamina lucida
81
mice, production of EGF may be affected by
estrogen and testosterone
82
Receptors for EGF may also occur in mitotically inactive cells such as
sweat ducts
83
Receptors for EGF are mainly found in
basal cell layer
84
Langerhans cell density decreases in
UV exposure, intrinsic aging, palms, soles, and anogenital region
85
collagen lamina densa.
Type 4
86
Langerhans cell density higher
face, neck, trunk and limbs 600 – 1000/sq mm
87
Anchoring filaments, rich in
laminin 5
88
lamina lucida thickness
is 30 to 60 nm
89
western blot identifies
Protein
90
northern blot identifies
RNA
91
southern blot identifies
DNA.
92
markers for hyperproliferative keratinocytes. They are found in skin disease such as psoriasis, warts, actinic keratoses, and SCC's.
K16 and K6
93
k2e/10
upper spinous and granular cell layers,
94
k 1/9 in
palmoplantar suprabasalar keratinocytes,
95
k4/13 expressed in
mucosal epithelium.
96
K6a/16 is expressed in
outer root sheath and in hyperproliferative keratinocytes
97
keratins expressed in the nail bed.
Keratins 6b & 17
98
Plectin is a member of the
plakin family
99
may be found in association with hair follicles or in some areas of modified skin such as the nipple/areola, labia minora, prepuce, vermilion border, and eyelids.
Sebaceous glands
100
CD6 and CD11c are expressed on all
mononuclear phagocytic cells in the dermis.
101
involucrin levels are increased in
In psoriasis,_
102
In psoriasis,_filaggrin and loricrin levels are
diminished.
103
Oxytalan fibers run____ from the DEJ within the superficial papillary dermis.
PERPENDICULAR
104
Elaunin fibers run __ in thin bands within the reticular dermis.
parallell
105
K16 and K6 are found in the
palms and soles.
106
K5 and K14 are found in
the basal layer.
107
K3 and K12 are found in the
suprabasilar cells of the cornea
108
cystathione synthase mutation causes what?
Homocystinuria
109
Lysyl hydroxylase is deficient in which type of EDS
Kyphoscoliosis type of EDS
110
Tenascin X is involved in what type of EDS?
Classical type EDS
111
dermatosparaxis type EDS has recessive mutations in the
type I collagen N-peptidase gene.
112
When do LC merkel cells melanocytes appear in embrology?
8-12 weeks
113
When does nail plate completely cover nail bed in emryogenesis?
15-20 weeks , second trimester
114
When does fetal basement membrane develop?
7 wk
115
What happens at 12 weeks embrogenesis?
1. DEJ form
2. Nail bed keratinizes, proximal nail fold formation
3. Collagen 3
116
Additional layerin plamoplantar epidermis known as ?
Stratum lucidum
117
When does mature epidermis become complete with interfollicular keratinization?
22-24 weeks
118
Which keratin on basic chr 12?
Type 2 k1-8 k71-80
119
Ornithine decarboxylase in expressed in which layer ?
Stratum basale
120
What protein defect causes
Vohwinkel
PPK with deafness
KID syndrome
Connexin 26
121
What protein defect causes
| Erythrokeratoderma variabilis?
Connexin 30.3/31
122
Connexin 30 ( gap junction protein) defect causes
Hidrotic ectodermal dysplasia
123
What condition with increased lamellar granules but structurally abnormal?
CIE
124
What conditions with decreased lamellar granules ?
Flegel, harlequin icthyosis
125
When does mature epidermis complete with interfollicular keratinization
22-24 wk
126
What causes naxos syndrome
Plakoglobin( desmosome)
127
What is the only common protein between adherens juction and desmosome
Plakoglobin
128
Defect in which protein causes carvajal syndrome
Dsmoplakin
129
Plakophilin defect causes
Ectodermal dysplasia with skin fragility
130
What structure makes up lamina lucida?
Anchoring filaments
131
What structure makes up lamina densa?
Anxhoring plaque
132
What structure makes up sublamina densa?
Anchoring fibril
133
What structure is made up of type 4 collagen, laminins, heparan sulfate?
Anchoring plques(lamina densa)
134
What structure is composed of type 7 collagen, fibrilin, type 1,3,4 collagen?
Anchoring fibril
135
Which layer of BMZ Made up of hemidesmosome
Basal keratinocyte,plasma membrane( attachment to ECM)
136
The hemidesmosomes are made up of ?
BPAG1, BPAG2, a6b4 integrin, plectin
137
BPAG1 is a member of
Plakin
138
BPAG2 is a member of
Collagen 17
139
Domain targeted by BP, labd ?
NC16A extracelular domain
140
Domain targeted by CP?
Carboxy terminal extracellular
141
CP target Ag?
BPag2
laminin 5
a6b4 integrin
142
Superantigens are ?Disease occurs when ?
Superantigens are polypeptides that activate large numbers of T cells
abnormally. Disease occurs when large amounts of proinflammatory cytokinesare produced.
143
Specific superantigens activate T cells bearing
specific amino acid sequences on the VB region of the T-cell receptor.
144
Toxic shock syndrome is a rapidly progressive, serious systemic illness
caused by superantigens produced by
superantigens produced by Saureus and group A Streptococcus.
145
Streptococcal TSS is usually associated with
severe necrotizing soft tissue infection.
146
Psoriasis presence of staphylococcal colonization and staphylococcal SAgs is associated
with more severe disease
147
Clindamycin why should it be included in the antibiotic regimen for Necrotizing soft tissue infection
Clindamycin has been shown to inhibit toxin production by both S aureus and GAS
148
Complement involved in both innate and adaptive
T
149
Innate immunity can bind to self antigens. T?
F . Only to non self
150
IL2, 4, 5, IFNy, TGFb part of cytokines for whichi immunity?
Adaptive
151
Cytokines of innate immunity are.
Il 1,10, 12, IFN a/b
152
What are cellular component of innate immunity
Macrophage NK cell mast cell eosinophil
153
Which pathway results in NFKb activation
TLR pathway
154
Which protein complex controls transcription of DNA
NFKB
155
Th2 cell mileiu
IL4,5,6,10
156
IL 2, 12, IFNy, TNF a belong to which T cell subtype ?
Th1
