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ID Review > Bacteriology Primer - IDBR > Flashcards

Bacteriology Primer - IDBR Flashcards

1
Q

What are the gram positive catalase positive organisms? [3]

A

Coag + and negative staph
Micrococcus
Kocuria

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2
Q

What are the gram positive catalase negative organisms? [2]

A

Enterococcus and strep

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3
Q

Which gram positive organisms are beta hemolytic on blood agar? [3]

A

Staphylococcus aureus
Streptococcus pyogenes
Groups B, C, F, G Streptococci

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4
Q

What gram positive organisms are PYR positive? [2]

A

Enterococcus species

Streptococcus pyogenes

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5
Q

Is staph aureus hemolytic?

A

Yes - Beta hemolytic

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6
Q

What gram positive organisms are alpha/gamma hemolytic? [3]

A

Enterococcus species
Viridans Streptococcus species
Streptococcus pneumoniae [alpha alone]

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7
Q

What gram positive organisms are beta hemolytic? [3]

A

Staphylococcus aureus
Streptococcus pyogenes
Groups B, C, F, G Streptococci

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8
Q

Gram stain shows gram positive cocci in clusters which is coagulase positive and beta hemolytic. Diagnosis

A

Staph aureus

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9
Q

Gram stain shows gram positive cocci in clusters which is coagulase negative. Diagnosis? [3]

A

Coag negative staph
Micrococcus species
Kocuria species

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10
Q

Gram stain shows gram + cocci in pairs and short chains. Ddx? [3]

A

Enterococcus species
Streptococcus pyogenes
Strep pneumo

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11
Q

Gram stain shows gram + cocci in pairs and long chains. Ddx? [2]

A

Groups B, C, F, G Streptococci

Viridans Streptococcus species

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12
Q

What is lancefield group A strep?

A

S. pyogenes

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13
Q

What is lancefield group B strep?

A

S. agalactiae

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14
Q

What is lancefield group C strep?

A

S. dysgalactiae subsp. dysgalactiae

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15
Q

What is lancfield group C, G, (A, L) strep?

A

S. dysgalactiae subsp. equisimilis

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16
Q

What is lance field group A, C, G, F strep?

A

S. anginosus group

17
Q

What is lance field group E, P, U, V strep?

A

S. porcinus

18
Q

Gram stain shows gram + cocci in pairs and short chains. PYR +, α‐ or ɣhemolytic. Dx?

A

Enterococcus

19
Q

Gram stain shows gram + cocci in pairs and short chains. PYR +, Beta hemolytic. Dx?

A

Streptococcus pyogenes

20
Q

Gram stain shows gram + cocci in pairs and short chains. PYR -, alpha hemolytic. Dx?

A

Streptococcus pneumoniae

21
Q

Gram stain shows gram + cocci in long chains, Catalase (‐), α‐ or ɣ‐ hemolytic. Dx?

A

Viridans Streptococcus

22
Q

Gram stain shows gram + cocci in long chains, Catalase (‐), beta hemolytic. Dx?

A

Groups B, C, F, G Streptococci

23
Q

What determines lance field groups?

A

Teichoic acid antigen.

24
Q

What do gram positive cocci in tetrads indicate on gram stain?

A

coagulase negative staphylococci or Micrococcus species

25
Q

What is the only PYR positive strep species?

A

Strep pyogenes

26
Q

Abiotrophia/Granulicatella require what to grow?

A

pyridoxal or cysteine

27
Q

What agar do Abiotrophia/Granulicatella grow on?

A

Chocolate agar

28
Q

What group is strep pneumonia a member of?

A

Streptococcus mitis

29
Q

What type of colonies do strep pneumonia form?

A

Donut shaped, raised edges, indented center

30
Q

What is the difference between Beta, alpha, and gamma hemolysis?

A

Beta - completely lyse RBCs leaving a clear zone of hemolysis around the colony
Alpha - incompletely lyse RBCs leaving greenish discoloration of the culture medium surrounding the colony
Gamma hemolytic - Do NOT hemolyze, AKA non-hemolytic

31
Q

What is the significance of optochin sensitivity?

A

Both Strep pneumonia and Strep viridian’s is alpha hemolytic. To differentiate this a disc inoculated with optochin is placed on the plate.
Strep pneumonia is inhibited
Strep viridans will grow

32
Q

What 5 groups of strep make up the virdans group?

A

mutans, anginosus (milleri), mitis, salivarius, bovis

–> Each group has multiple species in it.

ID Review (25 decks)

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