Bacterial Transformation

Question 1

What are the genes involved?

Answer 1

araC, GFP and bla

Question 2

What are the proteins involved?

Answer 2

arabinase, GFP and beta-lactamase

Question 3

What are the substrates involved?

Answer 3

luria broth, ampicillin, arabinose

Question 4

What is an operon?

Answer 4

Operons are ways to regulate the expression of genes

Question 5

What are inducible operons?

Answer 5

Gene is expressed when substrate levels are high and something else present needs the specific gene, like arabinose with arabinase

Question 6

What is a Repressible operon?

Answer 6

Gene is expressed when substrate levels are low

Question 7

What is a promoter?

Answer 7

Place at the beginning of the DNA strand where the RNA polymerase binds to in order to read the DNA

Question 8

What is a operator?

Answer 8

In between the promoter and genes, it blocks the RNA from reading unless repressor protein from operator is taken away

Question 9

How does operon-level gene regulation differ from epigenetic regulation?

Answer 9

It’s not really based on what’s present in the outside environment, but more of what proteins are present/not present in the body and what is expressed due to that

Question 10

What is the Antibiotic Selection Process?

Answer 10

Technique used to identify/isolate bacteria found in foreign DNA (cloned or recombinant) by using antibiotic resistance markers

Question 11

What is Transformation Efficiency?

Answer 11

How many cells pick up plasmid relative to how much plasmid you add in total