Bacteria Shape & Gram Stain

Question 1

Neisseria meningitidis gram stain, aerobic or anaerobic? Does it use maltose?

Answer 1

Gram negative diplococci, and utilizes maltose

Question 2

Neisseria gonorrheae morphology

Answer 2

Gram negative diplococci, and does not utilize maltose

Question 3

Moraxella gram stain, aerobic or anaerobic? Does it use maltose?

Answer 3

Gram negative diplococci, and does not utilize
maltose negative
Asacchrolytic - does not utilize sugars / carbohydrates
oxidase positive
catalase positive

Question 4

H. influenzae gram stain and shape?

Answer 4

Gram negative coccobacilli
Remember requires X factor & V factor to grow = Quadrant 3 and 4

Question 5

Bordetella pertussis gram stain and shape?

Answer 5

Gram negative coccobacilli

Question 6

Pasteurella gram stain and shape?

Answer 6

Gram negative coccobacilli

Question 7

Brucella gram stain and shape?

Answer 7

Gram negative coccobacilli

Question 8

Francisella tularensis stain and shape?

Answer 8

Gram negative coccobacilli

Question 9

Coryne bacterium diphtheria

Answer 9

Gram positive, curved or chinese letter arrangement, and catalase positive, aerobic

Question 10

Listeria monocytogenes

Answer 10

Gram positive, bacilli, aerobic

Question 11

Staphylococcus aureus

Answer 11

Gram positive round clusters, catalase positive, and coagulase positive, and mannitol salt +

Question 12

Micrococcus sp.

Answer 12

Gram positive round clusters , catalase positive, coagulase negative, oxidase positive, and bacitracin sensitive

Question 13

Staphylococcus saprophyticus

Answer 13

Gram positive round clusters, catalase positive, coagulase negative, oxidase negative, bacitracin resistant, and novobicin resistant, and urease positive

Question 14

Staphylococcus lugdunensis

Answer 14

Gram positive round clusters (staph), catalase positive, coagulase negative, oxidase negative, bacitracin resistant, Novobicin sensitive, Orn decarboxylase positive, TMP +, PYR positive

Question 15

Staphylococcus epidermidis

Answer 15

Gram positive round clusters or short chains
Catalase positive
Coagulase negative
Oxidase negative
Bacitracin Resistant
Novobicin sensitive

Question 16

Enterococcus sp.

Answer 16

Gram positive long chains (strep)
Catalase negative
Esculin positive
6.5% NaCl positive (can survive high salt)
PYR +

Question 17

Non-Enterococcus Group D Streptococci

Answer 17

Gram positive strep (long chains)
Catalase negative
Esculin positive
6.5% NaCl negative ( will not grow under high salt levels)
PYR negative

Question 18

Streptococcus agalactiae (Group B Streptococcus)

Answer 18

Gram positive strep (long chain)
Catalase negative
Esculin negative
Beta hemolysis
Bacitracin resistant
Hippurate Positive
CAMP Positive (synergism with S. aureus)

Question 19

Streptococcus pyogenes (Group A Strep)

Answer 19

Gram positive Strep
Catalase negative
Esculin negative
Beta hemolysis
Bacitracin sensitive
PYR Positive

Question 20

Viridans Strep

Answer 20

Gram positive Strep
Catalase negative
Esculine negative
Alpha hemolysis
Optochin resistant

Question 21

Streptococcus pneumoniae

Answer 21

Gram positive Strep
Catalase negative
Esculine negative
Alpha hemolysis
Optochin sensitive

Question 22

List two genus that are gram negative diplococci

Answer 22

Neisseria and Moraxella

Question 23

List the genus that are gram negative coccobacilli.

Answer 23

Haemophilus
Bordetella
Pasteurella
Brucella
Francisella

Question 24

List the genus of gram negative comma shaped that are oxidase positive

Answer 24

Campylobacter
Vibrio
Helicobacter

Question 25

List the genus of gram negative bacilli

Answer 25

Shigella
Yersinia
Salmonella
Proteus
Pseudomonas
Citrobacter
Serratia
Escherichia
Klebsiella
Enterobacter

Question 26

What biochemical test would differentiate proteus vulgaris and proteus mirabilis from each other?

Answer 26

Indole test. Vulgaris is indole positive. Mirabilis is indole negative.