Exam A Make Up Material

Question 1

True or False, Moraxella does ferment sugars like glucose and sucrose.

Answer 1

False, Moraxella is assacrolytic and does not ferment sugars at all.

Question 2

Is Moraxella DNase positive or negative?

Answer 2

Moraxella is DNAse positive

Question 3

What kind of atmospheric condition does campylobacter grow in?

Answer 3

Microaerophilic meaning it can grow in anaerobic conditions.

Question 4

Enterococcus has what kind of antibiotic resistance?

Answer 4

Vancomycin resistance (VRE)

Question 5

What biochemical test differentiates S. Pneumonia from Viridans group?

Answer 5

Optochin disk (P - Disk)

S. pneumoniae is susceptible to optochin disk
S. Viridans is resistant to optochin disk

Question 6

What antibiotic would differentiate staphylococcus from micrococcus?

Answer 6

Bacitracin (A-disk):
Micrococci. - Susceptible
Staphylococci - Resistant

Question 7

Is saprophyticus novobiocin (A-disk) resistant or sensitive?

Answer 7

Resistant

Question 8

What does the latex agglutination test detect?

Answer 8

Protein A and bound coagulate

Question 9

Is pyogenes susceptible to bacitracin (A-disk)?

Answer 9

Susceptible, The other lancefield groups are resistant to bacitracin

Question 10

Between Lancefield Group A and Group B, what are their results for PYR test?

Answer 10

Strep Pyogenes - PYR positive
Strep Aga - PYR negative

Question 11

Strep colonies are growing on your plate. What antibiotic disk would you use to differentiate pneumonia from pyogenes?

Answer 11

P disk and A disk. Pneumoniae is S to P disk. Pyogenes is S to A disk.

Question 12

The species and genus of Lancefield Group A is?

Answer 12

S. Pyogenes

Question 13

The genus and species for Lancefield Group B is?

Answer 13

S. Agalactiae

Question 14

List genus that are DNAse positive?

Answer 14

Aureus and Moraxella

Question 15

What are two differentiating biochemical tests for Strep agalactiae?

Answer 15

CAMP positive
Hippurate Positive

Question 16

What sugar does Neisseria gonorrhea ferment?

Answer 16

Glucose only

Question 17

What sugar does Neisseria meningitides ferment?

Answer 17

Glucose and maltose

Question 18

What Neisseria. Could be found in joint fluid?

Answer 18

N. gonorrhoeae

Question 19

What is the ideal media to culture G. Vaginalis?

Answer 19

Human blood tween

Question 20

What bacteria could grow on loeffler’s medium?

Answer 20

Corynebacterium. Spp.

Question 21

What is the TSI result for Erysipelothrix?

Answer 21

Gram positive bacilli
Catalase negative
Positive for H2S
Causes - Skin infection from fish

Question 22

How long can brucella culture continue growing?

Answer 22

I think 21 days will check

Question 23

What is the optimal temperature for campylobacter culture?

Answer 23

42C in a microaerophilic environment.

Question 24

What medias are good choices to cultuivate campylobacter if it is present?

Answer 24

Campy - BA, Charcoal selective medium, Campylobacter agar based blood free (CCDA)

Question 25

How do you differentiate B. anthraxis from other bacillus species?

Answer 25

B. anthracis is non-motile, gamma hemolysis (no hemolysis), susceptibility to penicillin, and gamma phages. It can be ruled out testing penicillin resistance. since B. anthracis is susceptible.

Question 26

What organism is responsible for whooping cough?

Answer 26

Bordetella pertussis

Question 27

What is the media of choice to isolate bordetella pertussis?

Answer 27

Charcoal blood agar, Regan lowe medium, or bordet - gengou agar

Question 28

What genus of bacteria are bile esculin positive?

Answer 28

Entero and listeria

Question 29

What biochemical reaction differentiates staph and strep bacteria?

Answer 29

Gram positive chains with catalase positive will be staphylococci Gram positive clusters with catalase negative will be streptococci Note: Staph aureus and intermedius are both coagulase and catalase positive

Question 30

What are the reagents in VP test?

Answer 30

KOH and alpha - naphthol

Question 31

How do you differentiate Salmonella paratyphi and Salmonella Typhi?

Answer 31

Paratyphi produces no H2S while Typhi produces a small amount of H2S

Question 32

What is prevotella's sensitivity to vancomycin, colistin, kanamycin, and presence of bile exculin?

Answer 32

Resistant to all antibiotic and no growth in the presence of bile esculin

Question 33

Tube coagulase tests for what kind of coagulase?

Answer 33

Free coagulase with rabbit plasma.

Question 34

How do you differentiate Streptococcus bovis (group D strep) from eneterococci?

Answer 34

Test specimen with the 6.5% NaCl test. Eneterococci are resistant but S. bovis is negative for 6.5% NaCl

Question 35

What are the reagents for 6.5% NaCl test?

Answer 35

Glucose and bromcresol purple (indicator). A positive test is yellow (from purple). A negative is a purple color.

Question 36

What is the reagent of PYR test?

Answer 36

N,N- dimethylaminocinnamaldehyde

Question 37

What is the reagent in MCAT disk?

Answer 37

bromo-chloro-indolyl butyrate, used to detect butyrate esterase. Important tool in differentiating M. catarrhalis which is positive but negative for Neisseria species.

Question 38

What is the most common Neisseria found in joint fluid?

Answer 38

Neisseria gonorrhoeae