Exam A Make Up Material Flashcards
True or False, Moraxella does ferment sugars like glucose and sucrose.
False, Moraxella is assacrolytic and does not ferment sugars at all.
Is Moraxella DNase positive or negative?
Moraxella is DNAse positive
What kind of atmospheric condition does campylobacter grow in?
Microaerophilic meaning it can grow in anaerobic conditions.
Enterococcus has what kind of antibiotic resistance?
Vancomycin resistance (VRE)
What biochemical test differentiates S. Pneumonia from Viridans group?
Optochin disk (P - Disk)
S. pneumoniae is susceptible to optochin disk
S. Viridans is resistant to optochin disk
What antibiotic would differentiate staphylococcus from micrococcus?
Bacitracin (A-disk):
Micrococci. - Susceptible
Staphylococci - Resistant
Is saprophyticus novobiocin (A-disk) resistant or sensitive?
Resistant
What does the latex agglutination test detect?
Protein A and bound coagulate
Is pyogenes susceptible to bacitracin (A-disk)?
Susceptible, The other lancefield groups are resistant to bacitracin
Between Lancefield Group A and Group B, what are their results for PYR test?
Strep Pyogenes - PYR positive
Strep Aga - PYR negative
Strep colonies are growing on your plate. What antibiotic disk would you use to differentiate pneumonia from pyogenes?
P disk and A disk. Pneumoniae is S to P disk. Pyogenes is S to A disk.
The species and genus of Lancefield Group A is?
S. Pyogenes
The genus and species for Lancefield Group B is?
S. Agalactiae
List genus that are DNAse positive?
Aureus and Moraxella
What are two differentiating biochemical tests for Strep agalactiae?
CAMP positive
Hippurate Positive
What sugar does Neisseria gonorrhea ferment?
Glucose only
What sugar does Neisseria meningitides ferment?
Glucose and maltose
What Neisseria. Could be found in joint fluid?
N. gonorrhoeae
What is the ideal media to culture G. Vaginalis?
Human blood tween
What bacteria could grow on loeffler’s medium?
Corynebacterium. Spp.
What is the TSI result for Erysipelothrix?
Gram positive bacilli
Catalase negative
Positive for H2S
Causes - Skin infection from fish
How long can brucella culture continue growing?
I think 21 days will check
What is the optimal temperature for campylobacter culture?
42C in a microaerophilic environment.
What medias are good choices to cultuivate campylobacter if it is present?
Campy - BA, Charcoal selective medium, Campylobacter agar based blood free (CCDA)
How do you differentiate B. anthraxis from other bacillus species?
B. anthracis is non-motile, gamma hemolysis (no hemolysis), susceptibility to penicillin, and gamma phages.
It can be ruled out testing penicillin resistance. since B. anthracis is susceptible.
What organism is responsible for whooping cough?
Bordetella pertussis
What is the media of choice to isolate bordetella pertussis?
Charcoal blood agar, Regan lowe medium, or bordet - gengou agar
What genus of bacteria are bile esculin positive?
Entero and listeria
What biochemical reaction differentiates staph and strep bacteria?
Gram positive chains with catalase positive will be staphylococci
Gram positive clusters with catalase negative will be streptococci
Note: Staph aureus and intermedius are both coagulase and catalase positive
What are the reagents in VP test?
KOH and alpha - naphthol
How do you differentiate Salmonella paratyphi and Salmonella Typhi?
Paratyphi produces no H2S while Typhi produces a small amount of H2S
What is prevotella’s sensitivity to vancomycin, colistin, kanamycin, and presence of bile exculin?
Resistant to all antibiotic and no growth in the presence of bile esculin
Tube coagulase tests for what kind of coagulase?
Free coagulase with rabbit plasma.
How do you differentiate Streptococcus bovis (group D strep) from eneterococci?
Test specimen with the 6.5% NaCl test. Eneterococci are resistant but S. bovis is negative for 6.5% NaCl
What are the reagents for 6.5% NaCl test?
Glucose and bromcresol purple (indicator). A positive test is yellow (from purple). A negative is a purple color.
What is the reagent of PYR test?
N,N- dimethylaminocinnamaldehyde
What is the reagent in MCAT disk?
bromo-chloro-indolyl butyrate, used to detect butyrate esterase. Important tool in differentiating M. catarrhalis which is positive but negative for Neisseria species.
What is the most common Neisseria found in joint fluid?
Neisseria gonorrhoeae
