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Clinical Micro 2 Spring 2023 > Exam A Make Up Material > Flashcards

Exam A Make Up Material Flashcards

1
Q

True or False, Moraxella does ferment sugars like glucose and sucrose.

A

False, Moraxella is assacrolytic and does not ferment sugars at all.

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2
Q

Is Moraxella DNase positive or negative?

A

Moraxella is DNAse positive

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3
Q

What kind of atmospheric condition does campylobacter grow in?

A

Microaerophilic meaning it can grow in anaerobic conditions.

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4
Q

Enterococcus has what kind of antibiotic resistance?

A

Vancomycin resistance (VRE)

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5
Q

What biochemical test differentiates S. Pneumonia from Viridans group?

A

Optochin disk (P - Disk)

S. pneumoniae is susceptible to optochin disk
S. Viridans is resistant to optochin disk

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6
Q

What antibiotic would differentiate staphylococcus from micrococcus?

A

Bacitracin (A-disk):
Micrococci. - Susceptible
Staphylococci - Resistant

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7
Q

Is saprophyticus novobiocin (A-disk) resistant or sensitive?

A

Resistant

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8
Q

What does the latex agglutination test detect?

A

Protein A and bound coagulate

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9
Q

Is pyogenes susceptible to bacitracin (A-disk)?

A

Susceptible, The other lancefield groups are resistant to bacitracin

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10
Q

Between Lancefield Group A and Group B, what are their results for PYR test?

A

Strep Pyogenes - PYR positive
Strep Aga - PYR negative

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11
Q

Strep colonies are growing on your plate. What antibiotic disk would you use to differentiate pneumonia from pyogenes?

A

P disk and A disk. Pneumoniae is S to P disk. Pyogenes is S to A disk.

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12
Q

The species and genus of Lancefield Group A is?

A

S. Pyogenes

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13
Q

The genus and species for Lancefield Group B is?

A

S. Agalactiae

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14
Q

List genus that are DNAse positive?

A

Aureus and Moraxella

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15
Q

What are two differentiating biochemical tests for Strep agalactiae?

A

CAMP positive
Hippurate Positive

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16
Q

What sugar does Neisseria gonorrhea ferment?

A

Glucose only

17
Q

What sugar does Neisseria meningitides ferment?

A

Glucose and maltose

18
Q

What Neisseria. Could be found in joint fluid?

A

N. gonorrhoeae

19
Q

What is the ideal media to culture G. Vaginalis?

A

Human blood tween

20
Q

What bacteria could grow on loeffler’s medium?

A

Corynebacterium. Spp.

21
Q

What is the TSI result for Erysipelothrix?

A

Gram positive bacilli
Catalase negative
Positive for H2S
Causes - Skin infection from fish

22
Q

How long can brucella culture continue growing?

A

I think 21 days will check

23
Q

What is the optimal temperature for campylobacter culture?

A

42C in a microaerophilic environment.

24
Q

What medias are good choices to cultuivate campylobacter if it is present?

A

Campy - BA, Charcoal selective medium, Campylobacter agar based blood free (CCDA)

25
Q

How do you differentiate B. anthraxis from other bacillus species?

A

B. anthracis is non-motile, gamma hemolysis (no hemolysis), susceptibility to penicillin, and gamma phages.
It can be ruled out testing penicillin resistance. since B. anthracis is susceptible.

26
Q

What organism is responsible for whooping cough?

A

Bordetella pertussis

27
Q

What is the media of choice to isolate bordetella pertussis?

A

Charcoal blood agar, Regan lowe medium, or bordet - gengou agar

28
Q

What genus of bacteria are bile esculin positive?

A

Entero and listeria

29
Q

What biochemical reaction differentiates staph and strep bacteria?

A

Gram positive chains with catalase positive will be staphylococci
Gram positive clusters with catalase negative will be streptococci

Note: Staph aureus and intermedius are both coagulase and catalase positive

30
Q

What are the reagents in VP test?

A

KOH and alpha - naphthol

31
Q

How do you differentiate Salmonella paratyphi and Salmonella Typhi?

A

Paratyphi produces no H2S while Typhi produces a small amount of H2S

32
Q

What is prevotella’s sensitivity to vancomycin, colistin, kanamycin, and presence of bile exculin?

A

Resistant to all antibiotic and no growth in the presence of bile esculin

33
Q

Tube coagulase tests for what kind of coagulase?

A

Free coagulase with rabbit plasma.

34
Q

How do you differentiate Streptococcus bovis (group D strep) from eneterococci?

A

Test specimen with the 6.5% NaCl test. Eneterococci are resistant but S. bovis is negative for 6.5% NaCl

35
Q

What are the reagents for 6.5% NaCl test?

A

Glucose and bromcresol purple (indicator). A positive test is yellow (from purple). A negative is a purple color.

36
Q

What is the reagent of PYR test?

A

N,N- dimethylaminocinnamaldehyde

37
Q

What is the reagent in MCAT disk?

A

bromo-chloro-indolyl butyrate, used to detect butyrate esterase. Important tool in differentiating M. catarrhalis which is positive but negative for Neisseria species.

38
Q

What is the most common Neisseria found in joint fluid?

A

Neisseria gonorrhoeae

39
Q
A

Clinical Micro 2 Spring 2023 (17 decks)

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