Bacillus & Listeria Flashcards
- What is the morphology of bacilli microscopically?
- What is the gram stain of bacilli?
- Rods
- Positive
True or false:
Bacilli have spores.
True
What is this organism?
Bacillus anthracis
What type of hemolysis does Bacilli use?
NONE
What does the Bacillus toxin bind to?
Protective antigen
What are the 2 incubation areas for Bacillus anthracis?
- Consistent alternation between wet and dry
- Soil high in nitrogen
How does Bacillus anthracis affect cattle?
Septicemia, toxemia, and death
How does Bacillus anthracis affect sheep?
Septicemia, toxemia, death
How does Bacillus anthracis affect horses?
Colic, edema of the head, neck, & shoulders
How does Bacillus anthracis affect swine?
Affects pharyngeal region
How does Bacillus anthracis affect dogs and cats?
Affects pharyngeal region
How can Bacillus anthracis affect a human being?
- Respiratory infection
- Malignant carbuncle
- Gastroenteritis
How did human beings become infected with Bacillus anthracis?
- Woolsorting
- Making drums/hides
Which species of Bacillus is penicillin suseptible?
Anthracis
How might you diagnosis Bacillus anthracis?
- Ear slice
- Do an impression smear
- Culture
- Typical colonies
- Penicillin suseptibility
- PCR
- What type of immunizing agents are there for animals?
- What about for humans?
- Modified live
- Killed strain
- How often must you get revaccinated?
- When does immunity typically wane?
- Anually
- Every 6 months
What are your drugs of choice in treating Bacillus anthracis in an animal?
- Penicillin
- Tetracyclin
How might an infection with Bacillus cereus be acquired?
Food poisoining
What might predispose an individual to a Bacillus subtilis infection?
- Heroin use
- Immunocomprimised
What problems can Listeria monocytogenes cause in a ruminant?
- Abortion
- CNS disease
What is the morphology of Listeria microscopically?
Gram positive flat rod (coccoidal)
How many toxins make up Bacillus anthracis?
What are they?
- Protective antigen
- Edema factor
- Lethal factor
Which toxins of Bacillus anthracis act as A-subunits?
- Edema factor
- Lethal factor
How does edema factor work in Bacillus anthracis?
Activates adenylate cyclase and interferes with cell function
How does the lethal factor work in Bacillus anthracis?
Proteolyticly cleaves the kinase involved in signal transduction in CD4+ T lymphocytes and fibroblasts. It inhibits IL-2 production and proliferative responses in the CD4+ T cells. Cleavage of the MAPK kinase in fibroblasts is apparently lethal to those cells.
What is the capsule of Bacillus anthracis composed of?
Poly-D-glutamic acid
To be affective, what must the Bacillus anthracis vaccine have in it?
Protective antigen
- Which animals does Listeria monocytogenes affect?
- What problems does it cause in each of these species?
- Cattle & sheep
- CNS problems in cattle and sheep; abortions in sheep
What type of hemolysis does Listeria monocytogenes use?
Narrow zone BETA hemolysis
List the laboratory results for Listeria monocytogenes in the following tests:
- H2S
- Catalase
- Litmus milk
- Motility
- H2S = negative
- Catalse = positive
- Litmus milk = rapid reduction
- Motility = at 22EC by peritrichous flagella
What is this organism?
Listeria monocytogenes
- In what pH does L. monocytogenes replicate?
- At which pH should silage be kept because of this?
- 5.5 - 9.0
- Below 5
List ubiquitous places for L. monocytogenes to be found?
- Soil
- Intestinal tracts (herbivores)
- Feces
- Silage
- Plant material
- Milk
Explain in detail how L. monocytogenes gets away from humoral immune responses in the body?
- Organism is ingested by a macrophage
- Listeriolysin O destructs the phagosome
- Listeris is released into the cytosol
- Surrounded by actin-like filaments from the host cell
- This structure forms a tail
- Facilitates movement to the cell membrane of macrophage
- Cell membrane evaginates
- Organism transfered to another macrophage
- Being an intracellular parasite, is NOT susceptible to killing by (1) antibody or (2) complement
Listerolysin O allows Listeria to be what type of parasite?
Falcultative intracellular parasite
What affects does L. monocytogenes have on cattle?
- Circling disease
- Unilateral facial paralysis
- Fever
- Blindness
- Head pressing
- Death
- Abortion
- Establishes a bacteremia or septicemia and localizes in the placentomes - that causes the death of the fetus
What affect does L. monocytogenes have on sheep and goats?
Same as in cattle:
Circling disease
Abortions
What affect does L. monocytogenes have on monogastrics / young ruminants?
- Septicemia
- Focal necrosis of the liver, spleen, LN
What might be your drugs of choice in treating L. monocytogenes?
- Ampicillin
- Tetracyclin
- Penicillin