BABS1201 W1-5 Labs

Question 1

What is the EPU for a 4X objective

Answer 1

25 micrometres

Question 2

What is EPU for 10x objective

Answer 2

10 micrometres

Question 3

What is the EPU for a 40x objective

Answer 3

2.5 micrometres

Question 4

What is the EPU for a 100x objective

Answer 4

1 micrometer

Question 5

What is the formula for diametre

Answer 5

Field number/objective magnification -

Question 6

What is the Biuret Test

Answer 6

normally blue but turns purple if there are proteins

Question 7

What is Benedict’s Test

Answer 7

Green > yellow > orange > brick red depending on how much sugar inside

Question 8

What is Lugol’s Test

Answer 8

Test for starch > add iodine solution > blue-black > starch present

Question 9

Oil Red O

Answer 9

If mixture turns red/pink there are fat droplets

Question 10

What does the dialysis tubing osmosis demo represent

Answer 10

Representative of a semi-permeable membrane

Question 11

What do cloudy tubes represent in Red Blood Cell Lysis in NaCl Solutions

Answer 11

No water movement in and out of the cell RBC intact, and therefore light enters scattered

Question 12

What do clear tubes represent in Red Blood Cell Lysis in NaCl Solutions

Answer 12

Decrease cloudiness → lysis → water swell in an RBC burst → haemoglobin scattered evenly hence the light can penetrate the solution → clear

Question 13

What happens if light is closer to the DPIP light solutions in photosynthesis

Answer 13

Closer the light → faster DPIP decolourisation → higher light intensity boosts photosynthesis.