B5: Sample Handling Flashcards

1
Q

Common samples collected

A
Urine
Feces
Blood
Tissue
Hair samples/skin scrapings
Fluid or discharge
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2
Q

Blood samples

A
Function of organs
Diagnose disease
Determine how medications affect body
Diagnose internal blood loss
Aid in toxin identification
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3
Q

Clotting Tubes

A

Red top
Tiger top (wax plug)
Yellow top/Serum separator tube (wax plug)

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4
Q

Anticoagulant Tube

A

Compound added to prevent formation of blood clots
Purple/Lavender top (EDTA)
Green top (heparin)

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5
Q

Serum

A

Fluid portion of blood (no cells)
No clotting factors remain once spun down
All in blood clot

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6
Q

Plasma

A

Fluid portion of blood (no cells)

Clotting factors remain due to anticoagulants

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7
Q

Whole Blood

A

Blood that has not been spun down

Has anticoagulants

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8
Q

Urine Samples

A

Identify urinary tract infections
Determine functions of organs (kidney and liver)
Diagnose disease (diabetes)
Identify abnormal crystals
Help in toxin identification (antifreeze)

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9
Q

Urine Collection Methods

A

Free catch
Manual expression
Cystocentesis
Urinary Catheter

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10
Q

Fecal Samples

A

Identify presence of internal parasites or parasite eggs
Diagnose parvovirus
Analyze function of digestive system

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11
Q

Tissues and Cells

A

Determine cause of illness or infection in organ or tissue
Identify possibility or type of cancer
Determine cause of death

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12
Q

Tissues and Cell Preservation

Cytology

A

Examination of cells under microscope

Sample spread out on slide and fixed

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13
Q

Tissue and Cell Preservation

Histology

A

Examination of a single layer of cells by pathologist

Sample preserved in formalin (10% formaldehyde solution)

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14
Q

Tissue and Cell Preservation

Bacterial Culture

A

Collected aseptically to prevent contamination

Preserved in sterile saline solution

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15
Q

Tissue and Cell Preservation

Special Handling

A

Zoonotic or reportable diseases

Rabies, brucellosis, TB, EIA (equine infectious anemia aka. Coggins)

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16
Q

Hair Samples and Skin Scrapings

A

Identify external parasites, bacteria or fungal infections
Identify parasites that live beneath skins surface
Identify reason for hair loss

17
Q

Fluids or Discharge

A

Debris/discharge from ear can determine source of ear infection
Ocular discharge can diagnose canine distemper
Vaginal swabs help determine correct time for breeding
Semen samples determine male fertility

18
Q

Fixing Solutions

A

Cells fixed in physical and chemical state to withstand testing
Volume of fixative must be 10x the volume of sample
Most common solution is formalin