B5: Sample Handling Flashcards
Common samples collected
Urine Feces Blood Tissue Hair samples/skin scrapings Fluid or discharge
Blood samples
Function of organs Diagnose disease Determine how medications affect body Diagnose internal blood loss Aid in toxin identification
Clotting Tubes
Red top
Tiger top (wax plug)
Yellow top/Serum separator tube (wax plug)
Anticoagulant Tube
Compound added to prevent formation of blood clots
Purple/Lavender top (EDTA)
Green top (heparin)
Serum
Fluid portion of blood (no cells)
No clotting factors remain once spun down
All in blood clot
Plasma
Fluid portion of blood (no cells)
Clotting factors remain due to anticoagulants
Whole Blood
Blood that has not been spun down
Has anticoagulants
Urine Samples
Identify urinary tract infections
Determine functions of organs (kidney and liver)
Diagnose disease (diabetes)
Identify abnormal crystals
Help in toxin identification (antifreeze)
Urine Collection Methods
Free catch
Manual expression
Cystocentesis
Urinary Catheter
Fecal Samples
Identify presence of internal parasites or parasite eggs
Diagnose parvovirus
Analyze function of digestive system
Tissues and Cells
Determine cause of illness or infection in organ or tissue
Identify possibility or type of cancer
Determine cause of death
Tissues and Cell Preservation
Cytology
Examination of cells under microscope
Sample spread out on slide and fixed
Tissue and Cell Preservation
Histology
Examination of a single layer of cells by pathologist
Sample preserved in formalin (10% formaldehyde solution)
Tissue and Cell Preservation
Bacterial Culture
Collected aseptically to prevent contamination
Preserved in sterile saline solution
Tissue and Cell Preservation
Special Handling
Zoonotic or reportable diseases
Rabies, brucellosis, TB, EIA (equine infectious anemia aka. Coggins)