B3.5-3.6 - Factors affecting enzymes (required practical)

Question 1

Describe the trend of the optimum temperature of enzymes

Answer 1

As temperature increases, the rate of reaction increases.
This is optimum at 37°C (around body temperature).
However at 40°C, the rate of reaction decreases rapidly and at around 54°C, the enzyme stops working and becomes denatured.

Question 2

What does denatured mean?

Answer 2

The active site is broken, the enzyme isn’t alive or dead but it can’t so it’s job since active site shape has changed.

Question 3

What is the effect of pH on enzymes?

Answer 3

Enzymes are also sensitive to pH . Changing the pH of its surroundings will also change the shape of the active site of an enzyme. This contributes to the folding of the enzyme molecule, its shape, and the shape of the active site. Changing the pH will affect the charges on the amino acid molecules.

Question 4

Carbohydrate catalysts

Answer 4

Carbohydrase

Question 5

Protein catalysts

Answer 5

Protease

Question 6

Lipid (fat) catalysts

Answer 6

Lipase

Question 7

What happens once the food molecules have been completely digested into soluble substrates?

Answer 7

Leave your small intestine and pass into your bloodstream