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B1-B3 > B1.1.3 - Light Microscopy > Flashcards

B1.1.3 - Light Microscopy Flashcards

1
Q

Who was the first Scientist to see cells?

A

Scientist Robert Hooke

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2
Q

When did Robert Hooke first see cells?

A

350 years ago

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3
Q

How does a light microscope work?

A

It passes light through an object placed on a slide on the stage, and then through the 2 glass lenses.

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4
Q

What are the 2 different glass lenses called?

A

Objective lens and eyepiece lens

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5
Q

What are the 2 knobs called?

A

Course focus knob

Fine focus knob

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6
Q

Which glass lens do you look through with your eye?

A

Eyepiece lens

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7
Q

When first viewing cells, what position should the stage be at?

A

Lowest position

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8
Q

When first viewing, which objective lens should you choose?

A

Lowest magnification lens

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9
Q

What is total magnification?

A

Eyepiece lens magnification

X

Objective lens magnification

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10
Q

Why would you stain cells?

A

Because most are colourless and cells are easier to observe with stains

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11
Q

What stain would you use to view the nucleus of an animal cell?

A

Methylene blue

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12
Q

What stain would you use to view plant cell nuclei?

A

Iodine

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13
Q

What stains bacterial cell walls?

A

Crystal violet

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14
Q

How would you apply a stain?

A

Place the cells on a glass slide
Add 1 drop of stain
Place a coverslip on top
Tap on it lightly with a pencil to remove air bubbles

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15
Q

Why would you use a plastic coverslip instead of a glass coverslip?

A

The glass coverslip may shatter under the pressure of the pencil

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16
Q

What is the maximum resolution for the light microscope?

A

0.2 micro meters

17
Q

What are the advantages to light microscope?

A 
They can view living specimens
They can view in colour
They are cheap
Easy to operate 
Portable
18
Q

What are the disadvantages of light microscopes?

A

They can’t view cells in as much detail as electron microscopes

B1-B3 (44 decks)

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