B1 - Cell Structure and Transport Flashcards
equation for magnification
magnification = image size / real size
How many micrometres (μm) in a millimetre (mm)?
1000μm = 1mm
What are the two categories of microscope?
Optical (light) and Electron
How do optical microscopes produce an image?
Lenses inside a microscope magnify an image
What is the maximum magnification of an optical microscope?
2000x
define resolution
The minimum distance between 2 distinct points on an image
What is the purpose of staining cells?
A technique that is used to enhance a specimen on a microscopic level using a dye.
What are two different dyes used when staining cells?
- iodine
- methylene blue
How do electron microscopes produce an image?
A beam of electrons is shot through a sample in a vacuum chamber which uses the wave-live properties to magnify an image.
What is the possible magnification of an electron microscope?
1,000,000x
What is the difference between a scanning electron microscope and an electron microscope?
A scanning electron microscope produces images that are in 3D, while an electron microscope produces images in 2D.
What are the 4 disadvantages of using an electron microscope?
- large
- expensive
- samples are dead because of the thin metallic coating applied
- not portable
What is a cell?
A cell is the simplest unit of a living thing.
What are the structures in living cells?
- Nucleus
- Ribosome
- Cell wall
- Cell membrane
- Cytoplasm
- Mitochondria
- Chloroplast
- Permanent vacuole
features of a prokaryotic cell
- simple and small
- 0.2μm - 2.0μm
- genetic material in a plasmid (strand)
- only organelles are ribosomes
- binary fission cell division
What are 2 advantages of electron microscopes?
- high magnification
- high resolution
What are organelles?
Organelles are subcellular structures with one or more specific jobs in the cell. e.g. not cytoplasm
features of an eukaryotic cell
- complex and large
- 5μm - 100μm
- genetic material in a membrane, nucleus
- mitosis cell division
define differentiation
Differentiation is the process when a cell changes
define specialisation
Specialisation is what a cell changes into after differentiation
define diffusion
Diffusion is a passive process where molecules move from an area of high concentration to an area of low concentration (moving down the concentration gradient).
When do molecules stop diffusing?
Molecules diffuse until equilibrium is reached.
What factors affect the speed of diffusion?
- the difference in concentration
- distance molecules have to travel
- temperature
define solute
a substance that is dissolved in a solution
define solvent
a substance that can dissolve another substance
define solution
a solute dissolved in a solvent