B)Inhibitors of peptidoglycan antibiotics

Question 1

beta lactam antibiotics structure

Answer 1

all contains beta lactam ring

Question 2

fn of beta lactam antibiotics

Answer 2

bind to enzymes involved in the crosslinking of peptidoglycan eg transpeptidase

Question 3

beta lactamases are

Answer 3

group of enzyme that hydrolyze beta lactam ring. this destroys the fn of these rings

Question 4

MRSA methicillin resistant Staphylococcus aureus

Answer 4

resistant to beta lactam antibiotics including methicillin. results in hard to treat infections. due to mecA which encodes an altered transpeptidase

Question 5

describe structure vancomycin structure

Answer 5

isolated from bacteria. large complex structure including peptide and disaccharide

Question 6

how does vancomycin work

Answer 6

too large to cross OM gram -ive. in gram +ive binds to d-ala d-aka of pentapeptide and prevents transcription

Question 7

vancomycin is used for

Answer 7

treatment of MRSA infections. last resort

Question 8

describe vancomycin resisitance

Answer 8

plasmid encoded VanA. VanA binds to inside of PM, it is a d-ala d-ala ligase. synthesizes various peptides that replace the d-ala d-ala, making it no longer a target for vancomycin