Assays for Immune Function Flashcards
CBC with differential
complete blood count
- number of cells
- morphology of cells
inflammatory markers
erythrocyte sedimentation rate and C reactive protein
check normal ranges of which antibodies
IgG, IgM, and IgA
check serum level of IgD only if
fever is present
normal quantity does not always mean normal function so assays for antibody function
titer to vaccine
isohemagglutinins
antibodies generated in response to polysacchargies of gut flora which corss-react with A or B blood group erythrocyte antigens
flow cytometry can be used to _____ different types of lymphocytes
quantitate
CD3 lymphocyte marker
t-cell
CD4 lymphocyte marker
helper T cells
CD8 lymphocyte marker
cytotoxic T cells
CD 19 lymphocyte marker
B cells
CD 16/56 lymphocyte marker
NK cells
UNCH lymphocyte marker tests
- Limited Lymphocyte Marker (CD3 CD4 CD8)
2. Complete (all markers listed above)
t cell function assays
in vitro and uses mitogens
3-6 days
24 hours add thymidine
the increased amount of thymidine take up in DNA replication = lymphocytes are replicating and functioning
cutaneous delayed type hypersensitivity
in vivo
ex. positive response to tetanus or candida
Very important to realize if patient has immune deficiency it would be difficult to run this test because it requires “positive response”
