Aseptic Laboratory/Suture and Instruments Flashcards
Asepsis
Prevent wound contamination by destroying organisms before they enter the wound
Antisepsis
killing bacteria after they have entered the wound
Disinfection
use of germicidal substances to kill most microorganisms
Sterilization
the process of destroying all microorganisms such as bacteria, fungi, and spores
What is the goal of aseptic surgical technique?
minimize contamination of surgical wounds
What is not sterile once surgery starts?
Your back neck shoulders axillae below waist
Physical sterilization
Thermal energy
Filtration
Radiation energy
Chemical sterilization
Plasma
Disinfectants
What is the most common method of sterilization?
Autoclave
What is the minimum standard for autoclaving?
250F
15 psi
13 min
What is the disadvantage of steam sterilization?
dulls sharp instruments
What is used for Chemical sterilization?
Ethylene Oxide
Gas Plasma
What does the effectiveness of Chemical Sterilization depend on?
Gas concentration
Temperature
Exposure time
Humidity (minimum of 35%)
What is disinfected with “Cold Disinfectants”?
Dental Instruments
Scopes
What is the shelf life of a sterile pack using Heat sealed paper/transparent plastic pouch?
1 years
What is the shelf life of a sterile pack using 2 layers of polyethylene + muslin?
9 months
What is the shelf life of a sterile pack using 2 layers of crepe paper + muslin?
10 weeks
What is the shelf life of a sterile pack using 2 layers of double wrap muslin?
8 weeks
What is the shelf life of a sterile pack using 1 layer of crepe paper?
3 weeks
Halsted’s Principles of Surgery
- Gentle Tissue Handling
- Accurate hemostasis
- Preservation of blood supply
- Strict aseptic technique
- Sutures tied without tension
- Accurate tissue apposition
- Obliteration of dead space
- Postoperative wound protection
Primary Hemorrhage
Bleeding during procedure
Intermediate delayed hemorrhage
within 24 hours of surgery
Secondary delayed hemorrhage
After 24 hours - usually the result of ineffective ligation
Gelform
Absorbable gelatin sponge Topical hemostatic agent derived from pig skin