Antibody Techniques Flashcards

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1
Q

What is ELISA?

A

Enzyme-linked immunosorbent assay.

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2
Q

What does ELISA do?

A

It uses antibodies to detect the presence of an antigen within a solution.

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3
Q

What can immunohistochemistry be used for?

A

To diagnose cancer as it can identify abnormal gene expression within cells.

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4
Q

What is bright-field microscopy commonly used to observe?

A
  • Whole organisms
  • Parts of organisms
  • Thin sections of dissected tissue
  • Individual cells
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5
Q

What does fluorescence microscopy use to bind to and visualise certain molecules or structures within a cell?

A

Specific fluorescence labels.

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6
Q

What is the purpose of aseptic technique?

A

To eliminate unwanted microbial contaminants when culturing microorganisms or cells.

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7
Q

What does aseptic technique involve?

A

The sterilisation of equipment and culture media by heat or chemical means and subsequent exclusion of microbial contaminants.

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8
Q

How can a microbial culture be started?

A

Using an inoculum of microbial cells on an agar medium, or in a broth with suitable nutrients. Animal cells are grown in medium containing growth factors from serum.

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9
Q

What are growth factors?

A

Proteins that promote cell growth and proliferation. Growth factors are essential for the culture of most animal cells.

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10
Q

What does plating out of a liquid microbial culture on solid media allow for?

A

The number of colony-forming units to be counted and the density of cells in the culture to be estimated.

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11
Q

What is often required to achieve a suitable colony count?

A

Serial dilution.

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12
Q

What is required to identify and count viable cells?

A

Vital staining.

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13
Q

What are monoclonal antibodies?

A

Stocks of antibodies with the same specificity.

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