Anemias Flashcards
Hb S, C, D, and E are differentiated thru?
Solubility
Mobility in the electrophoresis
Hemoglobinopathies are result due to the differences in the arrangement of what substance?
Amino acids in the polypeptide chains
Charge of hemoglobin molecules
Negative charge
Hemoglobin molecules migrate in what pole?
Anode or positive pole
In cellulose acetate membrane electrophoresis what Hb is the fastest? Slowest.
Fastest-Hb H and I
Slowest-Hb C, E, O and A2
pH of cellulose acetate membrane
8.4-8.6
Alkaline
Stain used in cellulose acetate membrane
Ponceau S
Used to differentiate Hb S, G and D
Citrate agar membrane
These are important agents in the production of hemolytic anemia
Hb S
Hb C
Hb E
Hb D
pH of citrate agar
6.2-6.4
Acidic
Glutamic acid on the sixth position of the beta chain is replaced by Valine
Hemoglobin S
Homozygous state of Hb S causes? Heterozygous causes.
Homo-sickle cell anemia
Hetero-sickle cell trait
Differentiates some hemoglobin variants that migrate together on cellulose acetate membrane
Citrate agar membrane
RBC become rigid and are trapped in capillaries
Sickle cell anemia
Sickle cell anemia is associated with?
Hypersplenism
Joint swelling
Kidney failure
Sever normocytic hemolytic anemia with polychromasia
Sickle cell anemia
In sickle cell anemia: ____ bilirubin and ____haptoglobin
Increase bilirubin
Decrease haptoglobin
In cellulose acetate membrane electrophoresis. Hb S migrates with?
Hb G and D
Diagnosis of Hb S is made after?
6 months of age. Time of beta gamma switch
Defect is inherited from one parent
Sickle cell trait
Sickle cell trait:
Concentration of adult hemoglobin
HbA (60%) + HbS (40%) and normal amount of Hb F and A2
Screening test for the detection of sickling hemoglobin
Dithionite tube test
In dithionite tube test what reagent used to lysed the red cells
Saponin
In dithionite tube test:
This removes oxygen from the test environment resulting to deoxygenated state
Sodium dithionite
Buffer used in Cellulose acetate membrane
Tris EDTA boric acid TEB
The precipitate formed is consist of this liquid crystal
Tactoids
Deoxygenates hemoglobin
Sodium metabisulfite
Under deoxygenated state Hb S causes the formation of?
Sickle cells
How many drops of sodium metabisulfite if there is normal Hb? How bout decrease Hb?
Normal- 2 drops
Decrease-1 drop
This is used to seal the glass slide in sodium metabisulfite test
Petroleum jelly
Positive result in sodium metabisulfite test
Holly leaf appearance
Concentration of adult hemoglobin in Hb CC disease
HbC (90%) + HbA2 (2%) + HbF (7%)
Holly leaf is found in what sickle cell?
Sickle cell trait
Glutamic acid on the sixth position is replaced by lysine
Hb C
Rhomboid, tetragonal or rod shaped crystals of dense staining hemoglobin that often distort the cell
Hb C crystal
Normocytic anemia with target cells
Hb CC
In cellulose acetate membrane electrophoresis Hb C migrates with?
Hb E, O, A2
Occurs in African-American and African population. With no production of Hb A
Hemoglobin C disease
Hb CC disease
Adult hemoglobin concentration in heterozygous Hb C disease
HbA (60%) + HbC (40%)
HbA2 and F normal
A double heterozygous condition where S and C are inherited
Hb SC disease
Mild anemia with microcytes
Hb E
Most common hemoglobinopathy in the US
Sickle cell trait
Less severe than sickle cell anemia but more severe that Hb C disease
Hb SC disease
Concentration of adult hemoglobin in Hb SC
HbS (50%) + HbC (50%) + HbF (7%)
Hb E migrates with?
Hb C, O, A2
Glycine replaced glutamic acid in the 121 position in the beta chain
Hb D
characterized by a reduction or total absence of synthesis of one or more of the globin chain of hemoglobin
Thalassemia
Total lack of beta or alpha chain
Thalassemia major
Lysine replaces glutamic acid at position 26th on the beta chain
Hb E
Production of alpha or beta at a decrease rate
Thalassemia minor
Three gene deletion
Hb H disease
Make the solution turbid
Tactoids
In the thalassemia syndrome erythrocytes indices indicate a
Microcytic and hypochromic anemia
Associated with the decrease production of alpha chains
Alpha thalassemia
Hb D migrates with?
Hb S and G
Decrease production of beta chain
Beta thalassemia
Single cell deletion
Silent carrier state
Four gene deletion
Hemoglobin bart’s
Hydrops fetalis
Composed of four beta chains
Hb H
Hb H supravital stain
Brilliant cresyl blue