amylase rp

Question 1

what is the firsts step

Answer 1

place iodine solution into each well of a spotting tile.

Question 2

what is step 2

Answer 2

take 3 test tubes. in the first place starch solution. in the second place amylase solution. in the third place ph buffer

Question 3

what is step 3

Answer 3

place all 3 test tubes In a water bath at 30 degrees and leave for 10 mins. then mix all 3 solutions into one and mix.

Question 4

what is step 4

Answer 4

start a timer and after 30s place one drop of the mixed solution into the first well. it should turn blue black. when the first well turns orange starch is no longer present