All Cells Arise From Other Cells Flashcards
Why do you use stain to look at cells?
Provides contrast
Stains chromosomes
Allow to see stage of mitosis
Why are cells squashed to be seen under a microscope?
To allow light to penetrate to make chromosome visible
Why are cells placed in a watch glass full of HCl before looking at under a microscope?
To fix and kill cells
Stops cells dividing so the stay in same stage of mitosis
Make cellulose cell wall more porus so stain absorbed easier
Why is the last 2mm of a root used to study mitosis?
End of root grows by producing more cells (mitosis)
Back of tip grows by elongating cells (less cells per unit area= less mitosis)
What is a mitotic index?
The proportion of cells undergoing mitosis
What stain is used to observe mitosis ?
Toluidene blue stain
What is mitosis important for ?
Growth
Repair
Reproduction
What two stages can the cell cycle be split into?
Interphase and metaphase
What are the sub sections of interphase called?
Growth 1
Synthesis
Growth 2
How much of the cell cycle is interphase?
90%
How long is the average cell cycle ?
24 hrs
What occurs during G1?
Cell prepares for DNA replication
Cell grows
Protein is synthesised
What occurs during synthesis phase?
DNA is replicated
What occurs during G2?
Organelles replicate
Cell grows (increased volume)
Protein synthesis
What are te stages of mitosis called?
Prophase
Metaphase
Anaphase
Telophase
What occurs during prophase?
Nucleolus and nuclear membrane break down
Chromosomes condense (are visible)
Spindle fibres form from centrioles at poles of cell
Spindle fibres attach to centromeres