Advanced Molecular Techniques Flashcards
Name 3 types of gene level DNA sequencing
- Southern Hybridisation
- Northern Hybridisation
- Microarray
- RT-PCR
- DNA fingerprinting
Which enzyme does RNA PCR use?
Reverse transcriptase
What primer sequence is used on cDNA in RT-PCR and why?
- Primer has T-tail
- Complementary mRNA has polyA tail
What are the 3 main steps in RT-PCR?
mRNA > cDNA > RT-PCR
What is the main use of microarray technology?
To compare the gene expression of two conditions or individuals with/without conditions
What is microarray technology a measure of?
Conditional gene expression
What do colours infer in microarray technology?
Colour demonstrates the ratios of cDNA that has bound from each sample
What are the 2 main steps in microarray technology involving mRNA?
- mRNA isolation
- Reverse transcriptase labelling
- RNA is copied and makes cDNA with a fluorescent tag
What type of microarray technology compares an affected genome with a healthy genome?
Array Comparative Genome Hybridisation
What does array comparative genome hybridisation highlight?
- Microdeletions
- Microduplications
What does DNA fingerprinting examine?
The non-coding part of the genome
What does DNA fingerprinting compare?
The allelic variation at each locus
What differs at each loci between individuals?
The number of mini-satellites
What advanced molecular technique is used to form DNA databases?
DNA fingerprinting
What is karyotyping?
Looks at the banding patterns in a full set of chromosomes