A&P 1 Chapter 3 (Lecture) [The Cell, Part 5] Flashcards

1
Q

Forms of DNA

A
  • Nucleosome
  • Chromatin
  • Chromosomes
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2
Q

Protein to package DNA

A

Histone

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3
Q

Central Dogma Steps

A
  1. Activate Gene
  2. Produce mRNA (transcription)
  3. Edit mRNA (RNA processing)
  4. Read mRNA and assemble protein (Translation)
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4
Q

Gene Activation

A
  • DNA uncoils and histones are removed
  • Start sequence is exposed for RNA polymerase to attach (known as TATA box)
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5
Q

Transcription

A
  • RNA polymerase runs along the DNA sequence. Does the following:
    • Separates DNA strands
      • Sense strand (actual gene)
      • Non- Sense Strand
    • Reads non-sense strand to make a copy of the sense strand.
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6
Q

RNA Processing

A
  • Removal of Introns
    • Unneeded base sequence
  • Splicing of Exons
    • Portion of mRNA coding for sequence of amino acids in a polypeptide
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7
Q

Translation

A
  • mRNA leaves nucleus and binds to ribosomes at start codon
  • tRNA delivers amino acids
    • Anticodon = three RNA nucleotides complementary to mRNA codon
  • Ribosomes form peptide bond between amino acid
  • Continues process until it reaches a stop codon.
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8
Q

Structures of the Nucleus

A
  • Nuclear Envelope
  • Nuclear Pores
  • Nucleoplasm
  • Nucleolus
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9
Q

Nuclear Envelope

A
  • Double Membrane
  • Perinuclear Space (In between membranes)
  • Nuclear Pores
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10
Q

Nuclear Pores

A

Account for about 10% of the surface of the nucleus, are passageways that permit chemical communication between the nucleus and the cytosol. Proteins at the pores regulate the movement of ions and small molecules, and neither proteins nor DNA can freely cross the nuclear envelope.

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11
Q

Nucleoplasm

A
  • Fluid inside nucleus
    • DNA
    • RNA
    • Nucleotides
    • Enzymes
    • Ions
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12
Q

Nucleolus

A

Site of ribosomal RNA synthesis and ribosomal subunit assembly

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13
Q

Nucleosome

A

DNA coiled around histones (proteins)

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14
Q

Chromatin

A

Loosely coiled DNA, no protein

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15
Q

Chromosomes

A

Tightly coiled DNA, no proteins

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16
Q

The Cell Cycle

A
  • Interphase
    • G1 (Growth)
    • S Phase (Synthesis)
    • G2 (Growth)
  • Mitosis
    • Prophase
    • Metaphase
    • Anaphase
    • Telophase
17
Q

Interphase

A
  • G1
    • Normal cell functions
    • Cell growth
    • Protein synthesis
  • S Phase
    • DNA replication
  • G2
    • Proteins synthesis
    • Mitosis preparation
18
Q

Prophase

A
  • Chromosomes become visible
  • Nuclear envelope and nucleolis disappears
  • Spindle Apparatus begins to form
  • Centrioles begin to separate
19
Q

Metaphase

A
  • Chromosomes line up at metaphase plate
  • Centrioles at opposite poles
  • Spindle apparatus fully formed
    • Each chromatid attached to spindle fiber by its kinetochore.
20
Q

Anaphase

A
  • Chromatids separate
    • Move toward opposite poles
  • Cell elongates
  • Two indentical sets of chromosomes (one at each pole) at end of anaphase
21
Q

Telophase

A
  • Chromosomes uncondense
  • Nuclear envelopes reform
  • Nucleoli reform
  • Cleavage furrow forms
22
Q

DNA Replication

A
  • DNA strands separate
  • DNA polymerases run along each strand
  • DNA polymerases use free nucleotides to produce complementary strand
  • Process happens at multiple places along DNA strands
23
Q
A

Prophase

24
Q
A

Metaphase

25
Q
A

Anaphase

26
Q
A

Telophase