9B: Fertilization

Question 1

Only 2 animals with site of semen deposition is in UTERUS?

the rest is in VAGINA

Answer 1

1. sow
2. mare

Question 2

STAGES of SPERM TRANSPORT

a. Presence of first sperms in oviduct after mating

b. These sperms are not involved with fertilization because of structural damage to them during rapid transport

Answer 2

Initial rapid transport

(mating
→ semen ejaculated
→ released to female reproductive tract)

Question 3

STAGES of SPERM TRANSPORT

a. Formed 6-12 hrs after semen deposition
b. Slowly transported sperms

Answer 3

establishment of sperm reservoir

(sperms released after initial rapid transport)

Question 4

Spermatozoa haploid in nature, needs ____ ____ so as not to be recognized as
foreign by female body

Answer 4

sperm reservoir

Question 5

STAGES of SPERM TRANSPORT

a. Goes to ampulla
b. Sperms can have higher percentage to fertilize ovum or ova

Answer 5

slow release of sperm

(from sperm reservoir to
oviduct)

Question 6

Only (2) animals with sperm reservoir is location in UTEROTUBAL JUNCTION

the rest is in CERVIX

Answer 6

1. sow
2. mare

Question 7

(2) animals with sperm fertilizing capacity is up to 90 hours

Answer 7

1. bitch
2. queen

Question 8

(3) animals with sperm fertilizing capacity is up to 24-48 hours

Answer 8

1. cow
2. ewe
3. sow

Question 9

ONLY animals with sperm fertilizing capacity is up to 5 DAYS

Answer 9

MARE

Question 10

junction between uterus and fallopian tube

Answer 10

Uterotubal junction

Question 11

length of time sperm is still viable and can fertilize the ovum

Answer 11

sperm fertilizing capacity

Question 12

activation of acrosome important for sperm to
penetrate ovum

Answer 12

SPERM CAPACITATION

Question 13

site of sperm capacitation

Answer 13

uterus to the ➔ isthmus of oviduct

Question 14

◆ Modification or removal of sperm surface components by oviductal & uterine fluids
◆ Depletion of sperm cholesterol & glycoproteins at sperm surfaces
◆ Alteration in glycosaminoglycans

This is a process of?

Answer 14

sperm capacitation

Question 15

◆ Modification or removal of sperm surface components by oviductal & uterine fluids
◆ Depletion of sperm cholesterol & glycoproteins at sperm surfaces
◆ Alteration in glycosaminoglycans

This is a process of?

Answer 15

sperm capacitation

Question 16

1. Fusion of plasma membrane and outer acrosomal membrane
2. Vesiculation → release of acrosomal membrane
3. Activation of different acrosomal enzymes

Answer 16

sperm capacitation

Question 17

(2) acrosomal enzyme

Answer 17

1. hyaluronidase
2. acrosin

Question 18

➔ Fusion of male & female gamete & forming a single cell zygote, then migrates to uterus
➔ Oocyte viable up to 18 hrs after ovulation

◆ Time the mating
● ovum/ova still viable
● Capacitated sperm to fertilize

Answer 18

FERTILIZATION

Question 19

site of fertilization

Answer 19

Ampulla of the oviduct

Question 20

In process of fertilization, migration of sperm between ______ _____

Answer 20

cumulus oophorus

Question 21

migration of sperm is aided by (2)

Answer 21

a. hyaluronidase (most animals)
b. aryl sulfatase (boar)

Question 22

As sperm migrates, and upon reaching ampulla, capacitated sperm will migrate between _____
and ___ ____ cells

Answer 22

cumulus &
corona radiata cells

Question 23

Migrations among cells surrounding the
ovum are aided by _____ enymes

Answer 23

acrosomal enzymes

Question 24

Process of fertilization:

a. Occurrence of acrosomal reaction
b. Occurs before or after sperm attaches to ZP3 receptors on zona pellucida

Answer 24

Passage of sperm to zona pellucida

Question 25

Process of fertilization:

In passage of sperm to zona pellucida, Eventually, sperm penetrates zona pellucida (glycoprotein layer) → then acrosomal reaction takes
place → attachment to _____ receptors

Answer 25

ZP3 receptors

Question 26

primary receptor for fertilization

Answer 26

ZP3 receptors

Question 27

secondary receptor maintaining
sperm/egg contact

Answer 27

ZP2 receptors

Question 28

Sperm penetration of zona ______

Answer 28

pellucida

Question 29

Penetration of ______ membrane

Answer 29

vitelline

Question 30

Zona pellucida reaction ensures ____ to prevent penetration of vitelline membrane of other sperms

Answer 30

monospermic fertilization

Question 31

a. Male + female pronucleus

b. Formation of zygote (diploid state restored)

c. Zygote divides into two-cell
i. Hours after sperm penetration:
1. Sow - 12-14
2. Ewe - 16-21
3. Cow - 20-24

d. Migrates to uterus for implantation

Answer 31

fusion of gametes