8.2 DNA Replication

Question 1

What is the function of helicase in DNA replication?

Answer 1

Helicase unwinds the DNA double helix, separating the two strands.

Question 2

True or False: DNA polymerase 3 is responsible for synthesizing the leading strand during DNA replication.

Answer 2

True

Question 3

Fill in the blank: DNA polymerase 1 is primarily responsible for _______.

Answer 3

removing RNA primers and replacing them with DNA.

Question 4

Which enzyme seals the nicks in the DNA backbone after replication?

Answer 4

Ligase

Question 5

Multiple Choice: Which enzyme is NOT involved in DNA replication?

Answer 5

Ligase (is involved, but not the answer; correct answer is a distractor, e.g., ‘RNA polymerase’)

Question 6

What is the role of DNA polymerase 3 in the replication process?

Answer 6

It synthesizes new DNA strands by adding nucleotides complementary to the template strand.

Question 7

True or False: Both DNA polymerase 1 and 3 can synthesize DNA in the 5’ to 3’ direction.

Answer 7

True

Question 8

What is the primary difference between DNA polymerase 1 and DNA polymerase 3?

Answer 8

DNA polymerase 1 has exonuclease activity for proofreading and replacing RNA primers, while DNA polymerase 3 is the main enzyme for DNA synthesis.

Question 9

Fill in the blank: The process of _______ involves the synthesis of a new DNA strand using an existing strand as a template.

Answer 9

DNA replication

Question 10

What is the significance of the leading and lagging strands in DNA replication?

Answer 10

The leading strand is synthesized continuously, while the lagging strand is synthesized in short fragments known as Okazaki fragments.