8.2 Flashcards
Leading strand
It goes from 5’ to 3’. It is also continuously.
Lagging strand
It goes from 5’ to 3’. It is discontinuous because DNA can only be added in the 5’ to 3’.
Semi-discontinuous
It is because of the way the leading and lagging strand function.
Semiconservative replication
When DNA is replicated, one stranded is the parental while the other one is newly synthesized strand.
Okazaki fragments
It is the fragment produce by lagging strand.
Helicase
Unzips the DNA, break the hydrogen bonds.
DNA Polymerase I
Remove the RNA primers and replace them with DNA nucleotide.
DNA Polymerase III
It add nucleotide to the 3” end of growing DNA.
Ligase
It completes the Okazaki fragments.
Primase
Provides a starting point for DNA synthesis.
Order of events in DNA replication
- DNA is separate. 2. RNA primer is lay down. 3. DNA nucleotide bases are added. 4. Okazaki fragment are glue together. 5. RNA primer is released by DNA.
Explain Meselson and Stahl’s experimental setup and what was concluded from the results
They grew E-coli bacteria in heavy nitrogen. Then they would switch to light nitrogen. It was concluded that DNA was semi-conservative.
What prime does DNA get add on to?
It is add on the 3’ end.
What are the difference between DNA Polymerase III and I
III is main DNA builder and 1000 bases per second.
I is editing, repair, and primer removal and does 20 base per second.
