8.2 Flashcards
leading strand
the parent strand that is added to continuously.
lagging strand
the parent strand that is added to non-continuously
semi-discontinuous
A mode of DNA replication in which one new strand is synthesized continuously, while the other is synthesized discontinuously as Okazaki fragments
semiconservative replication
When replicating, DNA base pairs are broken apart in order to form two new strands.
Okazaki fragments
DNA fragments created on the lagging strand
Helicase
breaks the hydrogen bonds apart
DNA polymerase I
Replaces RNA primer with DNA
DNA polymerase III
aligns free nucleotide with their base partners
Ligase
Connects fragments left after DNA polymerase I to the rest of the strand
Primase
Generates an RNA primer that serves as a starting point for DNA polymerase III
Order of events:
Helicase separates strands; Primase constructs an RNA primer; DNA polymerase III builds off the primer and builds the rest of the chain; DNA polymerase I replaces the RNA primer with DNA; Ligase connects the DNA fragments left after DNA polymerase I.
Meselson-Stahl experiment
An experiment in which it was determined that DNA uses semi-conservative replication