7TH PRETEST

Question 1

Formalin pigment can be removed from tissue sections by treatment with: *

a) 10% hydrochloric acid in 70% alcohol
b) 10% nitric acid in 70% alcohol
c) 10% sulfuric acid in 70% alcohol
d) 10% ammonium hydroxide in 70% alcohol

Answer 1

d) 10% ammonium hydroxide in 70% alcohol

Question 2

To prevent the formation of formalin pigment in tissues, formalin should be: *

a) heated
b) cooled
c) buffered
d) acidified

Answer 2

c) buffered

Question 3

Relating to nerve cells, chromatolysis refers to the loss of: *
a) axons
b) Nissl substance
C) dendrites
d) myelin

Answer 3

b) Nissl substance

Question 4

The most consistently reliable technique for demonstrating fungi in tissues is the: *

a) PAS
b) Gridley method for fungi
c) Gomori methenamine silver (GMS)
d) Ziehl-Neelsen

Answer 4

c) Gomori methenamine silver (GMS)

Question 5

The recommended fixative for tissue suspected of containing spirochetes is: *

a) 10% neutral buffered formalin
b) Bouin solution
c) Zenker solution
d) Helley fluid

Answer 5

a) 10% neutral buffered formalin

Question 6

Pigment that is present on the surface of cells but not within them is probably: *

a) endogenous
b) hematogenous
c) hepatogenous
d) artifact

Answer 6

d) artifact

Question 7

Tumors of the adrenal medulla are called pheochromocytomas and to affect their demonstration should be fixed in: *
a) 95% alcohol
b) Orth's fluid
C) B-5 solution
d) 10% buffered fomalin

Answer 7

b) Orth’s fluid

Question 8

Nissl substance can be demonstrated with which of the following stains: *

a) thionin and cresyl echt violet
b) eosin and phloxine
c) silver nitrate and gold sublimate
d) orange G and methyl blue

Answer 8

a) thionin and cresyl echt violet

Question 9

Permitting slides to remain in alcohols after the OG or EA counterstains:

a. will cause removal of the cytoplasmic color
b. should be avoided to prevent cell dehydration
c. has no effect on staining quality
d. allows adequate hydration of cells

Answer 9

a. will cause removal of the cytoplasmic color

Question 10

Individual virus particles cannot be seen by light microscopy, but can be visualized by: *

a) phase microscopy
b) polarizing microscopy
c) electron microscopy
d) fluorescence microscopy

Answer 10

c) electron microscopy

Question 11

A technique for demonstrating calcium, wherein sections immersed in silver nitrate solution are exposed to bright light, is the: *

a) Schmorl
b) von Kossa
c) dopa oxidase
d) alizarin red S

Answer 11

b) von Kossa

Question 12

The term “argentaffin” literally refers to a reaction wherein cells have the ability to reduce a salt of this metal: *

a) chromium
b) lithium
c) copper
d) silver

Answer 12

d) silver

Question 13

The point of contact between an axon of one neuron and the dendrite of another neuron is called: *

a) link
b) synapse
c) attachment
d) contact

Answer 13

b) synapse

Question 14

The second largest portion of the brain is the: *

a) cerebrum
b) cerebellum
c) pons
d) medulla

Answer 14

b) cerebellum

Question 15

A component of nerve tissue that can be demonstrated by gold and silver impregnation techniques is: *
a) neurofibrils
b) Nissl substance
C) dendrites
d) myelin

Answer 15

a) neurofibrils

Question 16

The pigment commonly known as “wear and tear pigment” or “brown atrophy” is: *

a) hemofuchsin
b) ceroid
c) lipofuchsin
d) hemosiderin

Answer 16

c) lipofuchsin

Question 17

The largest portion of the brain is the: *
a) medulla
b) midbrain
C) cerebellum
d) cerebrum

Answer 17

d) cerebrum

Question 18

Lung sections showing granulomatous inflammation on H&E stained sections are commonly stained with at least two techniques to demonstrate the presence of: *

a) fungi or acid-fast organisms
b) fibrosis or acid-fast organisms
c) fungi or muscle
d) fungi or fibrosis

Answer 18

a) fungi or acid-fast organisms

Question 19

The nerve process carrying electrical impulses away from the cell body is called an: *
a) neuron
b) dendrite
C) synapse
d) axon

Answer 19

d) axon

Question 20

Select the example of an exogenous pigment: *

a) argentaffm
b) melanin
c) chromaffin
d) carbon

Answer 20

d) carbon

Question 21

Cells and fibers comprising specialized connective tissue of the central nervous system are: *

a) oocytes
b) chondrocytes
c) neuroglia
d) phagocytes

Answer 21

c) neuroglia

Question 22

Pigments in this organ are considered to be some of the most difficult to differentiate on routine staining: *

a) lung
b) liver
c) spleen
d) kidney

Answer 22

b) liver

Question 23

The nerve process carrying nerve impulses toward the cell body nucleus is called an: *

a) dendrite
b) axon
c) neuron
d) ganglion

Answer 23

a) dendrite

Question 24

The fixative of choice for demonstration of urate crystals is: *

a) neutral buffered formalin
b) absolute alcohol
c) Bouin’s
d) Zenker’s

Answer 24

b) absolute alcohol

Question 25

The cytotechnologist informs you that there is no staining color present in the slides from the Papanicolaou run. The most likely cause is: *

a. exhausted stains
b. insufficient staining time
c. prolonged time in clearing reagent
d. incomplete removal of fixative prior to staining

Answer 25

a. exhausted stains