6TH PRETEST

Question 1

In silver impregnation techniques, use of improperly cleaned glassware results in:

a. absence of silver staining
b. nonselective silver precipitation
c. inadequate counterstaining
d. inability to tone

Answer 1

b. nonselective silver precipitation

Question 2

The most frequent fiber found in connective tissue is:

a. reticulin
b. elastin
c. collagen
d. myelin

Answer 2

c. collagen

Question 3

Select the sudanophilic connective tissue component:

a. elastin
b. reticulin
c. adipose
d. cartilage

Answer 3

c. adipose

Question 4

In staining methods for reticulin, potassium permanganate, phosphomolybdic acid, and periodic acid function as:

a. reducers
b. oxidizers
c. sensitizers
d. toners

Answer 4

b. oxidizers

Question 5

The hematoxylin of choice for nuclear staining in lengthy and acidic trichrome methods is:

a. alum hematoxylin
b. phosphotungstic acid hematoxylin
c. chrome-alum hematoxylin
d. iron hematoxylin

Answer 5

d. iron hematoxylin

Question 6

Select the proteins that form the thick and thin filaments of skeletal muscle fibers:

a. fibrin and fibrinogen
b. collagen and tropocollagen
c. actin and myosin
d. thrombin and prothrombin

Answer 6

c. actin and myosin

Question 7

In the Verhoeff-Van Gieson technique for elastic fibers, collagen stains:

a. red
b. blue
c. black
d. yellow

Answer 7

a. red

Question 8

A modified connective tissue structure which nourishes and supports epithelial cells is called:

a. plica circularis
b. muscularis mucosa
c. adventitia
d. basement membrane

Answer 8

d. basement membrane

Question 9

This histological feature is unique to cardiac muscle:

a. cross-striations
b. peripherally located nuclei
c. intercalated discs
d. non-branching fibers

Answer 9

c. intercalated discs

Question 10

A phosphotungstic acid-hematoxylin solution will ripen naturally over several weeks, but can be ripened for immediate use by adding:

a. potassium permanganate
b. aluminum sulfate
c. acetic acid
d. alcohol

Answer 10

a. potassium permanganate

Question 11

Two eosinophilic tissue components that may be difficult to distinguish morphologically are:

a. elastic and skeletal muscle
b. cartilage and ground substance
c. collagen and smooth muscle
d. peripheral nerve and cardiac muscle

Answer 11

c. collagen and smooth muscle

Question 12

Select a commonly used connective tissue procedure that stains collagen blue:

a. Masson trichrome
b. Van Gieson
c. Movat pentachrome
d. Gomori one-step trichrome

Answer 12

a. Masson trichrome

Question 13

Stains for simple lipids are performed on:

a. routinely processed paraffin sections
b. frozen sections
c. celloidin processed sections
d. glycol methacrylate sections

Answer 13

b. frozen sections

Question 14

The phosphotungstic acid-hematoxylin (PTAH) stain is useful for demonstrating:

a. edema fluid
b. muscle striations
c. ground substance
d. reticulin net

Answer 14

b. muscle striations

Question 15

With the Verhoeff-Van Gieson technique, elastic fibers stain:

a. red to purple
b. blue-black to black
c. yellow to brown
d. blue to blue-green

Answer 15

b. blue-black to black

Question 16

The amorphous transparent gel-like material that forms the bulk of extracellular content in connective tissue is known as:

a. basal lamina
b. lamina propria
c. mesenchyme
d. ground substance

Answer 16

d. ground substance

Question 17

A preferred fixative for demonstrating simple fats in tissues is:

a. Zenker
b. Helly
c. Schaudinn
d. 10% neutral buffered formalin (NBF)

Answer 17

d. 10% neutral buffered formalin (NBF)

Question 18

Although the periodic acid-Schiff (PAS) is not a conventional stain for reticulin, under certain conditions these fibers are PAS-positive and they are also:

a. stainable with neutral red
b. stainable with orcein
c. argyrophilic
d. basophilic

Answer 18

c. argyrophilic

Question 19

In the Masson trichrome method, muscle fibers are colored:

a. red
b. blue
c. yellow
d. green

Answer 19

a. red

Question 20

Another name for fat cells is:

a. APLD cells
b. myocytes
c. adipocytes
d. histiocytes

Answer 20

c. adipocytes

Question 21

Elastic fibers have an affinity for:

a. sudan
b. indigo
c. brazilin
d. orcein

Answer 21

d. orcein

Question 22

Using the Mallory phosphotungstic acid-hematoxylin (PTAH) method, skeletal muscle striations stain:

a. blue
b. red
c. black
d. yellow

Answer 22

a. blue

Question 23

The PTAH stain is useful in the diagnosis of a rhabdomyosarcoma, as this tumor is a malignant neoplasm of:

a. smooth muscle
b. skeletal muscle
c. epithelium
d. glands

Answer 23

b. skeletal muscle

Question 24

Muscle that histologically contains cytoplasmic cross-striations and has nuclei located at the edge of the fibers is classified as:

a. smooth
b. visceral
c. skeletal
d. cardiac

Answer 24

c. skeletal

Question 25

A malignant neoplasm of connective tissue origin is classified as a/an:

a. carcinoma
b. sarcoma
c. adenoma
d. leiomyoma

Answer 25

b. sarcoma