1ST PRETEST Flashcards
The most important step in the preparation of high-quality microscopic slides of tissue is:
a. ensuring adequate fixation
b. selecting the tissue processor
c. centering sections on the slides
d. determining the melting point of the paraffin
a. ensuring adequate fixation
Dehydration refers to the removal of:
a. Alcohol
b. Water
c. Paraffin
d. Xylene
b. Water
How many milliliters of water would be required to prepare 4500 mL of 15% formalin?
a. 3825
b. 2812
c. 1688
d. 675
a. 3825
The histology supervisor gets a telephone call in the middle of the night from security staff at their institution. The security staff tells the supervisor that housekeeping staff accidentally spilled a small quantity of xylene in the histology laboratory. No one is working in the laboratory at that time at night. The security officer wants information about clean up. The supervisor should tell the security officer to:
a. Call another institution for instructions
b. Consult the standard operations procedure manual
c. Find a book on laboratory safety in the institution’s medical library
d. Not call them in the middle of the night - it could have waited until the morning
b. Consult the standard operations procedure manual
The type of microtome used for routine paraffin sectioning is a(n):
a. Rocking
b. Rotary
c. Sliding
d. Ultramicrotome
b. Rotary
The term “biopsy” means the excision of tissue:
a. Measuring less than 1 cm in depth
b. Measuring less than 1.5 cm in diameter
c. Removed by electrocautery
d. For the purpose of examination and diagnosis
d. For the purpose of examination and diagnosis
A tumor or new growth largely made up of nerve cells is a/an:
a. Fibroma
b. Lipoma
c. Neuroma
d. Osteoma
c. Neuroma
The terms “squamous,” “cuboidal,” and “columnar” describe cells that have their origin in which of the following types of tissue?
a. Bone
b. Connective Tissue
c. Epithelium
d. Muscle
c. Epithelium
Connective tissue is the most diverse type of tissue in the body and is composed of:
a. Fibrous material only
b. Few cells and much extracellular material
c. Acellular gelatinous substance
d. Numerous cells with little intercellular tissue
b. Few cells and much extracellular material
Hemosiderin, hemoglobin, and bile pigment are classified as:
a. Endogenous pigments
b. Artifact pigments
c. Exogenous pigments
d. Extraneous pigments
a. Endogenous pigments
Touch-preps involve pressing a:
a. Finger against the tissue to remove excess fluid
b. Fresh-cut tissue surface against a glass slide
c. Tissue down into a cassette to make it fit
d. Sample to determine its density
b. Fresh-cut tissue surface against a glass slide
Cytology is the study of:
a. Cells
b. Cultures
c. Tissue
d. Morphology
a. Cells
The breakdown of tissue due to enzyme activity is called:
a. Polymerization
b. Putrefaction
c. Autolysis
d. osmosis
c. Autolysis
The effect of acid decalcification is most noticeable in the staining of:
a. Collagen
b. Cytoplasm
c. Nuclei
d. Osteocytes
c. Nuclei
The recommended temperature of the oven used for drying paraffin sections should be adjusted to:
a. 10-20°C
b. 20-30°C
c. 50-60°C
d. 70-80°C
c. 50-60°C
Which type of cytology specimen should NOT be submitted to the laboratory fresh?
a. F’NA
b. GYN
c. pleural fluid
d. urine
b. GYN
“Areolar connective tissue” is also known as: *
a. Mineralized connective tissue
b. Dense connective tissue
c. Loose connective tissue
d. Specialized connective tissue
c. Loose connective tissue
Adequate fixation of a kidney biopsy for diagnostic electron microscopy is obtainable with all of the following except:
a. Initial fixation in neutral buffered formalin, subsequent fixation in glutaraldehyde.
b. Initial fixation in carson-millonig buffered formalin
c. Initial fixation in 95 percent alcohol followed by fixation in glutaraldehyde.
d. Initial fixation in glutaraldehyde.
c. Initial fixation in 95 percent alcohol followed by fixation in glutaraldehyde.
Relating to nerve cells, chromatolysis refers to the loss of:
a. Axons
b. Nissl substance
c. Dendrites
d. Myelin
b. Nissl substance
A physical agent used in fixation is: *
a. Hydrogenation
b. Heat
c. Coagulation
d. Emulsification
b. Heat
Select the staining procedure most suitable for demonstrating general tissue morphology:
a. Hematoxylin and eosin
b. Masson trichrome
c. Periodic acid-schiff
d. Wilder reticulin
a. Hematoxylin and eosin
Generally, differentiation in regressive staining:
a. Releases dyes first from components that have strong bonds
b. Relies on precise deposition of dyes on specific tissue components
c. Removes dyes from tissue components that are desired to be unstained
d. Results in dyes creating poor nuclear detail
c. Removes dyes from tissue components that are desired to be unstained
The optimum tissue thickness for routine processing in the standard tissue cassette is:
a. 1 mm
b. 2mm
c. 3mm
d. 4mm
c. 3mm
Which of the following embedding procedures involves first infiltrating the tissue with one medium, and then embedding it in another?
a. Double
b. Resin
c. Routine
d. vacuum
a. Double
The volume of fixing fluid employed should be:
a. 2 - 5 times that of the tissue
b. 6 - 9 times that of the tissue
c. 10 - 14 times that of the tissue
d. 15 - 20 times that of the tissue
d. 15 - 20 times that of the tissue
