1ST PRETEST

Question 1

The most important step in the preparation of high-quality microscopic slides of tissue is:

a. ensuring adequate fixation
b. selecting the tissue processor
c. centering sections on the slides
d. determining the melting point of the paraffin

Answer 1

a. ensuring adequate fixation

Question 2

Dehydration refers to the removal of:

a. Alcohol
b. Water
c. Paraffin
d. Xylene

Answer 2

b. Water

Question 3

How many milliliters of water would be required to prepare 4500 mL of 15% formalin?

a. 3825
b. 2812
c. 1688
d. 675

Answer 3

a. 3825

Question 4

The histology supervisor gets a telephone call in the middle of the night from security staff at their institution. The security staff tells the supervisor that housekeeping staff accidentally spilled a small quantity of xylene in the histology laboratory. No one is working in the laboratory at that time at night. The security officer wants information about clean up. The supervisor should tell the security officer to:

a. Call another institution for instructions
b. Consult the standard operations procedure manual
c. Find a book on laboratory safety in the institution’s medical library
d. Not call them in the middle of the night - it could have waited until the morning

Answer 4

b. Consult the standard operations procedure manual

Question 5

The type of microtome used for routine paraffin sectioning is a(n):

a. Rocking
b. Rotary
c. Sliding
d. Ultramicrotome

Answer 5

b. Rotary

Question 6

The term “biopsy” means the excision of tissue:

a. Measuring less than 1 cm in depth
b. Measuring less than 1.5 cm in diameter
c. Removed by electrocautery
d. For the purpose of examination and diagnosis

Answer 6

d. For the purpose of examination and diagnosis

Question 7

A tumor or new growth largely made up of nerve cells is a/an:

a. Fibroma
b. Lipoma
c. Neuroma
d. Osteoma

Answer 7

c. Neuroma

Question 8

The terms “squamous,” “cuboidal,” and “columnar” describe cells that have their origin in which of the following types of tissue?

a. Bone
b. Connective Tissue
c. Epithelium
d. Muscle

Answer 8

c. Epithelium

Question 9

Connective tissue is the most diverse type of tissue in the body and is composed of:

a. Fibrous material only
b. Few cells and much extracellular material
c. Acellular gelatinous substance
d. Numerous cells with little intercellular tissue

Answer 9

b. Few cells and much extracellular material

Question 10

Hemosiderin, hemoglobin, and bile pigment are classified as:

a. Endogenous pigments
b. Artifact pigments
c. Exogenous pigments
d. Extraneous pigments

Answer 10

a. Endogenous pigments

Question 11

Touch-preps involve pressing a:

a. Finger against the tissue to remove excess fluid
b. Fresh-cut tissue surface against a glass slide
c. Tissue down into a cassette to make it fit
d. Sample to determine its density

Answer 11

b. Fresh-cut tissue surface against a glass slide

Question 12

Cytology is the study of:

a. Cells
b. Cultures
c. Tissue
d. Morphology

Answer 12

a. Cells

Question 13

The breakdown of tissue due to enzyme activity is called:

a. Polymerization
b. Putrefaction
c. Autolysis
d. osmosis

Answer 13

c. Autolysis

Question 14

The effect of acid decalcification is most noticeable in the staining of:

a. Collagen
b. Cytoplasm
c. Nuclei
d. Osteocytes

Answer 14

c. Nuclei

Question 15

The recommended temperature of the oven used for drying paraffin sections should be adjusted to:

a. 10-20°C
b. 20-30°C
c. 50-60°C
d. 70-80°C

Answer 15

c. 50-60°C

Question 16

Which type of cytology specimen should NOT be submitted to the laboratory fresh?

a. F’NA
b. GYN
c. pleural fluid
d. urine

Answer 16

b. GYN

Question 17

“Areolar connective tissue” is also known as: *

a. Mineralized connective tissue
b. Dense connective tissue
c. Loose connective tissue
d. Specialized connective tissue

Answer 17

c. Loose connective tissue

Question 18

Adequate fixation of a kidney biopsy for diagnostic electron microscopy is obtainable with all of the following except:

a. Initial fixation in neutral buffered formalin, subsequent fixation in glutaraldehyde.
b. Initial fixation in carson-millonig buffered formalin
c. Initial fixation in 95 percent alcohol followed by fixation in glutaraldehyde.
d. Initial fixation in glutaraldehyde.

Answer 18

c. Initial fixation in 95 percent alcohol followed by fixation in glutaraldehyde.

Question 19

Relating to nerve cells, chromatolysis refers to the loss of:

a. Axons
b. Nissl substance
c. Dendrites
d. Myelin

Answer 19

b. Nissl substance

Question 20

A physical agent used in fixation is: *

a. Hydrogenation
b. Heat
c. Coagulation
d. Emulsification

Answer 20

b. Heat

Question 21

Select the staining procedure most suitable for demonstrating general tissue morphology:

a. Hematoxylin and eosin
b. Masson trichrome
c. Periodic acid-schiff
d. Wilder reticulin

Answer 21

a. Hematoxylin and eosin

Question 22

Generally, differentiation in regressive staining:

a. Releases dyes first from components that have strong bonds
b. Relies on precise deposition of dyes on specific tissue components
c. Removes dyes from tissue components that are desired to be unstained
d. Results in dyes creating poor nuclear detail

Answer 22

c. Removes dyes from tissue components that are desired to be unstained

Question 23

The optimum tissue thickness for routine processing in the standard tissue cassette is:

a. 1 mm
b. 2mm
c. 3mm
d. 4mm

Answer 23

c. 3mm

Question 24

Which of the following embedding procedures involves first infiltrating the tissue with one medium, and then embedding it in another?

a. Double
b. Resin
c. Routine
d. vacuum

Answer 24

a. Double

Question 25

The volume of fixing fluid employed should be:

a. 2 - 5 times that of the tissue
b. 6 - 9 times that of the tissue
c. 10 - 14 times that of the tissue
d. 15 - 20 times that of the tissue

Answer 25

d. 15 - 20 times that of the tissue