5TH PRETEST Flashcards
The use of mounting media makes stained tissue components more visible by:
a. altering the density of the tissue
b. changing the pH of the dyes
c. matching the refractive index of the tissue
d. removing the xylene from the slide
c. matching the refractive index of the tissue
Which of the following terms are used to describe bone?
a. cancellous and compact
b. glial and astrocytic
c. reticular and collagenous
d. smooth and striated
a. cancellous and compact
The pathologist has requested a Feulgen procedure on a lymph node. The staining sequence should be:
a. hydrochloric acid, sulfuric acid rinses, Schiff, light green
b. hydrochloric acid, Schiff, sulfirous acid, light green
c. periodic acid, Schiff, water rinses, light green
d. Schiff, hydrochloric acid, light green
b. hydrochloric acid, Schiff, sulfirous acid, light green
Differential staining of nuclei and cytoplasm with Giemsa staining is an example of:
a. apochromasia
b. hyperchromasia
c. metachromasia
d. polychromasia
d. polychromasia
Nontoxic, basic stains that are suspended in sterile water and injected into a living animal are referred to as:
a. amphoteric
b. anionic
c. in vitro
d. vital
d. vital
A hematoxylin solution that is commonly used progressively and very rarely used regressively is:
a. Delafield
b. Ehrlich
c. Harris
d. Mayer
d. Mayer
- A compound containing a benzene ring with other atomic groupings associated with color is called a/an:
a. auxochrome
b. chromogen
c. chromophore
d. dye
b. chromogen
Microscopic differentiation of tissue components in unstained tissue sections is difficult. Consequently, routine stains are used to:
a. distinguish only the nuclei
b. stain the cytoplasm only
c. demonstrate the tissue components uniformly
d. differentiate between cytoplasm and nuclei
d. differentiate between cytoplasm and nuclei
An example of an iron hematoxylin is:
a. Delafield
b. Harris
c. Mayer
d. Weigert
d. Weigert
The refractive index of resinous mounting media used for stained sections should be:
a. 0.52 to 0.85
b. 1.30 to 1.41
c. 1.53 to 1.54
d. 1.73 to 1.90
c. 1.53 to 1.54
Mordants are generally classified as a/an:
a. acid
b. gas
c. metal
d. oxidizer
c. metal
Generally, acid dyes are differentiated in solutions that are weakly:
a. acidic
b. amphoteric
c. basic
d. neutral
c. basic
An amphoteric substance is one that can act as an:
a. acid or base
b. acid or oxidizer
c. base or salt
d. oxidizer or salt
a. acid or base
Which of the following is a method that differentially demonstrates DNA as green and RNA as red?
a. Feulgen reaction
b. Gomori trichrome
c. methyl green-pyronin
d. PAS-light green
c. methyl green-pyronin
A dye with negatively charged auxochromes is which type of dye?
a. acid
b. amphoteric
c. basic
d. polychromatic
a. acid
A stain using a silver salt that requires a developer is known as which of the following reaction types?
a. argentaffin
b. arginin
c. argyria
d. argyrophil
d. argyrophil
In the Feulgen reaction for nucleic acids, slides are placed in a 1 N (normal) solution of hydrochloric acid to promote:
a. alkalinity
b. autolysis
c. hydrolysis
d. oxidation
c. hydrolysis
An H&E of a patient’s tissue reveals brown, crystalline pigment over areas of red blood cells. To remove this pigment prior to re-staining, it is necessary to treat the sections in:
a. aqueous lithium carbonate
b. iodine and sodium thiosulfate
c. potassium permanganate and oxalic acid
d. saturated alcoholic picric acid
d. saturated alcoholic picric acid
Which of the following is an example of a synthetically produced dye?
a. aniline blue
b. carmin
c. hematoxylin
d. indigo blue
a. aniline blue
The structure in the cell nucleus that stains strongly with basic dyes is called:
a. chromatin
b. cytomembrane
c. lipofuscin
d. RNA
a. chromatin
Which of the following dyes is a known human carcinogen?
a. cochineal
b. hematoxylin
c. orcein
d. pararosaniline
d. pararosaniline
Which of the following chemicals functions as an oxidizer in some hematoxylin solutions?
a. acetic acid
b. aluminum ammonium sulfate
c. ammonium hydroxide
d. potassium permanganate
d. potassium permanganate
Picric acid, eosin, and tartrazine are examples of which type of dyes?
a. basic
b. cytoplasmic
c. fluorescent
d. natural
b. cytoplasmic
Which of the following is the mordant salt used to make the hematoxylin solutions most commonly used in the routine H&E stain?
a. aluminum
b. copper
c. iron
d. tungsten
a. aluminum
An acidic or basic salt added to a solution to prevent a change in pH is called a/an:
a. buffer
b. catalyst
c. differentiator
d. oxidizer
a. buffer
