5TH PRETEST

Question 1

The use of mounting media makes stained tissue components more visible by:

a. altering the density of the tissue
b. changing the pH of the dyes
c. matching the refractive index of the tissue
d. removing the xylene from the slide

Answer 1

c. matching the refractive index of the tissue

Question 2

Which of the following terms are used to describe bone?

a. cancellous and compact
b. glial and astrocytic
c. reticular and collagenous
d. smooth and striated

Answer 2

a. cancellous and compact

Question 3

The pathologist has requested a Feulgen procedure on a lymph node. The staining sequence should be:

a. hydrochloric acid, sulfuric acid rinses, Schiff, light green
b. hydrochloric acid, Schiff, sulfirous acid, light green
c. periodic acid, Schiff, water rinses, light green
d. Schiff, hydrochloric acid, light green

Answer 3

b. hydrochloric acid, Schiff, sulfirous acid, light green

Question 4

Differential staining of nuclei and cytoplasm with Giemsa staining is an example of:

a. apochromasia
b. hyperchromasia
c. metachromasia
d. polychromasia

Answer 4

d. polychromasia

Question 5

Nontoxic, basic stains that are suspended in sterile water and injected into a living animal are referred to as:

a. amphoteric
b. anionic
c. in vitro
d. vital

Answer 5

d. vital

Question 6

A hematoxylin solution that is commonly used progressively and very rarely used regressively is:

a. Delafield
b. Ehrlich
c. Harris
d. Mayer

Answer 6

d. Mayer

Question 7

7. A compound containing a benzene ring with other atomic groupings associated with color is called a/an:
   a. auxochrome
   b. chromogen
   c. chromophore
   d. dye

Answer 7

b. chromogen

Question 8

Microscopic differentiation of tissue components in unstained tissue sections is difficult. Consequently, routine stains are used to:

a. distinguish only the nuclei
b. stain the cytoplasm only
c. demonstrate the tissue components uniformly
d. differentiate between cytoplasm and nuclei

Answer 8

d. differentiate between cytoplasm and nuclei

Question 9

An example of an iron hematoxylin is:

a. Delafield
b. Harris
c. Mayer
d. Weigert

Answer 9

d. Weigert

Question 10

The refractive index of resinous mounting media used for stained sections should be:

a. 0.52 to 0.85
b. 1.30 to 1.41
c. 1.53 to 1.54
d. 1.73 to 1.90

Answer 10

c. 1.53 to 1.54

Question 11

Mordants are generally classified as a/an:

a. acid
b. gas
c. metal
d. oxidizer

Answer 11

c. metal

Question 12

Generally, acid dyes are differentiated in solutions that are weakly:

a. acidic
b. amphoteric
c. basic
d. neutral

Answer 12

c. basic

Question 13

An amphoteric substance is one that can act as an:

a. acid or base
b. acid or oxidizer
c. base or salt
d. oxidizer or salt

Answer 13

a. acid or base

Question 14

Which of the following is a method that differentially demonstrates DNA as green and RNA as red?

a. Feulgen reaction
b. Gomori trichrome
c. methyl green-pyronin
d. PAS-light green

Answer 14

c. methyl green-pyronin

Question 15

A dye with negatively charged auxochromes is which type of dye?

a. acid
b. amphoteric
c. basic
d. polychromatic

Answer 15

a. acid

Question 16

A stain using a silver salt that requires a developer is known as which of the following reaction types?

a. argentaffin
b. arginin
c. argyria
d. argyrophil

Answer 16

d. argyrophil

Question 17

In the Feulgen reaction for nucleic acids, slides are placed in a 1 N (normal) solution of hydrochloric acid to promote:

a. alkalinity
b. autolysis
c. hydrolysis
d. oxidation

Answer 17

c. hydrolysis

Question 18

An H&E of a patient’s tissue reveals brown, crystalline pigment over areas of red blood cells. To remove this pigment prior to re-staining, it is necessary to treat the sections in:

a. aqueous lithium carbonate
b. iodine and sodium thiosulfate
c. potassium permanganate and oxalic acid
d. saturated alcoholic picric acid

Answer 18

d. saturated alcoholic picric acid

Question 19

Which of the following is an example of a synthetically produced dye?

a. aniline blue
b. carmin
c. hematoxylin
d. indigo blue

Answer 19

a. aniline blue

Question 20

The structure in the cell nucleus that stains strongly with basic dyes is called:

a. chromatin
b. cytomembrane
c. lipofuscin
d. RNA

Answer 20

a. chromatin

Question 21

Which of the following dyes is a known human carcinogen?

a. cochineal
b. hematoxylin
c. orcein
d. pararosaniline

Answer 21

d. pararosaniline

Question 22

Which of the following chemicals functions as an oxidizer in some hematoxylin solutions?

a. acetic acid
b. aluminum ammonium sulfate
c. ammonium hydroxide
d. potassium permanganate

Answer 22

d. potassium permanganate

Question 23

Picric acid, eosin, and tartrazine are examples of which type of dyes?

a. basic
b. cytoplasmic
c. fluorescent
d. natural

Answer 23

b. cytoplasmic

Question 24

Which of the following is the mordant salt used to make the hematoxylin solutions most commonly used in the routine H&E stain?

a. aluminum
b. copper
c. iron
d. tungsten

Answer 24

a. aluminum

Question 25

An acidic or basic salt added to a solution to prevent a change in pH is called a/an:

a. buffer
b. catalyst
c. differentiator
d. oxidizer

Answer 25

a. buffer