3RD PRETEST

Question 1

After completion of decalcification, the specimen should be placed directly in:

a. Acetone
b. Alcohol
c. Fixative
d. water

Answer 1

d. water

Question 2

To ensure adequate processing, and yet safely decrease the time needed on the tissue processor, one can:

a. Reuse reagents all week
b. Start in higher percentages of dehydrant
c. Skip clearing stages
d. Use agitation at all stages

Answer 2

d. Use agitation at all stages

Question 3

A clearing agent must be miscible with:

a. Dehydrants and infiltrating media
b. Fixatives and dehydrants
c. Fixatives and infiltrating media
d. Water and dehydrants

Answer 3

a. Dehydrants and infiltrating media

Question 4

Methyl alcohol is often used as a dehydrant for:

a. Blood smears
b. Cytology smears
c. Frozen sections
d. Touch preps

Answer 4

d. Touch preps

Question 5

How much 95% alcohol must be used to prepare 1000 mL of 70% alcohol? (Round to the nearest whole number.

a. 136mL
b. 316 mL
c. 665 mL
d. 737 mL

Answer 5

d. 737 mL

Question 6

Which of the following is a proper disposal method for organic solvents (toluene, xylene, etc.

a. Pour down a sink, flushing with plenty of water.
b. Collect in a waste container for future incineration or distillation.
c. Pour into a deep pit in the ground.
d. Collect in old bottles, and store in vacant building.

Answer 6

b. Collect in a waste container for future incineration or distillation.

Question 7

The thickness of specimens to be decalcified should be:

a. 1 to 2 mm
b. 3 to 4 mm
c. 5 to 6 mm
d. 7 to 8 mm

Answer 7

b. 3 to 4 mm

Question 8

What is the usual time needed for complete infiltration of paraffin into a 3 mm thick piece of tissue, without the use of vacuum? *

a. 1 to 2 hours
b. 2 to 3 hours
c. 3 to 4 hours
d. 4 to 5 hours

Answer 8

b. 2 to 3 hours

Question 9

Which of the following are universal solvents?

a. Benzene and Butanol
b. Cellosolve and Methanol
c. Dioxane and Tetrahydrofuran
d. Ethanol and Xylene

Answer 9

c. Dioxane and Tetrahydrofuran

Question 10

If the clearing agent is cloudy, it probably is contaminated with:

a. Absolute alcohol
b. Bacteria
c. Water
d. Yeast

Answer 10

c. Water

Question 11

Which of the following is probably the best all-round substitute for ethanol for processing tissues? *

a. Acetone
b. Butanol
c. Isopropanol
d. Methanol

Answer 11

c. Isopropanol

Question 12

How often should the acid solution in an ion-exchange resin decalcifying system be changed?

a. Twice a day
b. Daily
c. Once or twice a week
d. Does not need to be changed

Answer 12

d. Does not need to be changed

Question 13

Acid solutions remove which of the following salts?

a. Calcium
b. Lithium
c. Potassium
d. Sodium

Answer 13

a. Calcium

Question 14

The lab needs to process tissue faster. Which of the following can be done easily and still give good results?

a. Add vacuum to 15 mm Hg at each station
b. Increase heat to 70°C at each station
c. Use butanol instead of ethanol
d. Infiltrate with a harder paraffin

Answer 14

a. Add vacuum to 15 mm Hg at each station

Question 15

Of the following, the identification number of the specimen should be marked on the cassette or label with a(an):

a. Ink pen
b. Laundry marker
c. Lead pencil
d. Wax pencil 1

Answer 15

c. Lead pencil

Question 16

The time needed for infiltration of paraffin is most dependent upon the:

a. Fixative used
b. Style of processor
c. Type of tissue
d. Number of cassettes

Answer 16

c. Type of tissue

Question 17

Which of the following are used as dehydrants only?

a. Acetone, Chloroform, Tetrahydrofuran
b. Butanol, Ethanol, Methanol
c. Carbon tetrachloride, Isopropanol, Toluene
d. Cellosolve, Dioxane, Methyl salicylate

Answer 17

b. Butanol, Ethanol, Methanol

Question 18

During microtomy, tissue is found to be difficult to section. The tissue is soft and mushy. To correct this in the future, change the processing schedule by: *

a. Decreasing time in formalin
b. Increasing time in dehydrant
c. Decreasing time in clearant
d. Decreasing time in paraffin

Answer 18

b. Increasing time in dehydrant

Question 19

Prolonged dehydration in higher grades of alcohol will render the specimen:

a. Hard
b. Macerated
c. Porous
d. toxic

Answer 19

a. Hard

Question 20

Why should the alcohols on the automated tissue processor be changed on a regular basis?

a. The alcohols become saturated with bile from the gallbladder specimens, which can be absorbed by the other tissues.
b. Too great a concentration of formalin in the alcohols can create a potentially explosive situation.
c. Gram negative organisms can grow in alcohol left too long on the processor.
d. The alcohols can absorb moisture and become dilute.

Answer 20

d. The alcohols can absorb moisture and become dilute.

Question 21

Which of the following will speed up the rate of decalcification, yet not cause harm to the tissue?

a. Increase the percentage of acid
b. Decrease the temperature of the decal fluid
c. Agitate the solution
d. Gross a thicker specimen.

Answer 21

c. Agitate the solution

Question 22

The maximum thickness of tissue which will allow adequate clearing in xylene in routine processing is:

a. 1-2 mm
b. 3-4 mm
c. 5-6 mm
d. 7-8 mm

Answer 22

b. 3-4 mm

Question 23

Which of the following are used as clearants only?

a. Acetone, Methyl salicylate, Toluene
b. Amy1 acetate, Methyl benzoate, Tetrahydrofuran
c. Benzene, Carbon tetrachloride, Cedarwood oil
d. Chloroform, Isopropanol, Xylene

Answer 23

c. Benzene, Carbon tetrachloride, Cedarwood oil

Question 24

Which of the following is considered the best routine dehydrant?

a. Acetone
b. Ethanol
c. Isopropanol
d. Methanol

Answer 24

b. Ethanol

Question 25

The process of saturating tissue with the medium that will later be used for embedding is called:

a. Clearing
b. Dehydration
c. Fixation
d. infiltration

Answer 25

d. infiltration