3RD PRETEST Flashcards
After completion of decalcification, the specimen should be placed directly in:
a. Acetone
b. Alcohol
c. Fixative
d. water
d. water
To ensure adequate processing, and yet safely decrease the time needed on the tissue processor, one can:
a. Reuse reagents all week
b. Start in higher percentages of dehydrant
c. Skip clearing stages
d. Use agitation at all stages
d. Use agitation at all stages
A clearing agent must be miscible with:
a. Dehydrants and infiltrating media
b. Fixatives and dehydrants
c. Fixatives and infiltrating media
d. Water and dehydrants
a. Dehydrants and infiltrating media
Methyl alcohol is often used as a dehydrant for:
a. Blood smears
b. Cytology smears
c. Frozen sections
d. Touch preps
d. Touch preps
How much 95% alcohol must be used to prepare 1000 mL of 70% alcohol? (Round to the nearest whole number.
a. 136mL
b. 316 mL
c. 665 mL
d. 737 mL
d. 737 mL
Which of the following is a proper disposal method for organic solvents (toluene, xylene, etc.
a. Pour down a sink, flushing with plenty of water.
b. Collect in a waste container for future incineration or distillation.
c. Pour into a deep pit in the ground.
d. Collect in old bottles, and store in vacant building.
b. Collect in a waste container for future incineration or distillation.
The thickness of specimens to be decalcified should be:
a. 1 to 2 mm
b. 3 to 4 mm
c. 5 to 6 mm
d. 7 to 8 mm
b. 3 to 4 mm
What is the usual time needed for complete infiltration of paraffin into a 3 mm thick piece of tissue, without the use of vacuum? *
a. 1 to 2 hours
b. 2 to 3 hours
c. 3 to 4 hours
d. 4 to 5 hours
b. 2 to 3 hours
Which of the following are universal solvents?
a. Benzene and Butanol
b. Cellosolve and Methanol
c. Dioxane and Tetrahydrofuran
d. Ethanol and Xylene
c. Dioxane and Tetrahydrofuran
If the clearing agent is cloudy, it probably is contaminated with:
a. Absolute alcohol
b. Bacteria
c. Water
d. Yeast
c. Water
Which of the following is probably the best all-round substitute for ethanol for processing tissues? *
a. Acetone
b. Butanol
c. Isopropanol
d. Methanol
c. Isopropanol
How often should the acid solution in an ion-exchange resin decalcifying system be changed?
a. Twice a day
b. Daily
c. Once or twice a week
d. Does not need to be changed
d. Does not need to be changed
Acid solutions remove which of the following salts?
a. Calcium
b. Lithium
c. Potassium
d. Sodium
a. Calcium
The lab needs to process tissue faster. Which of the following can be done easily and still give good results?
a. Add vacuum to 15 mm Hg at each station
b. Increase heat to 70°C at each station
c. Use butanol instead of ethanol
d. Infiltrate with a harder paraffin
a. Add vacuum to 15 mm Hg at each station
Of the following, the identification number of the specimen should be marked on the cassette or label with a(an):
a. Ink pen
b. Laundry marker
c. Lead pencil
d. Wax pencil 1
c. Lead pencil
The time needed for infiltration of paraffin is most dependent upon the:
a. Fixative used
b. Style of processor
c. Type of tissue
d. Number of cassettes
c. Type of tissue
Which of the following are used as dehydrants only?
a. Acetone, Chloroform, Tetrahydrofuran
b. Butanol, Ethanol, Methanol
c. Carbon tetrachloride, Isopropanol, Toluene
d. Cellosolve, Dioxane, Methyl salicylate
b. Butanol, Ethanol, Methanol
During microtomy, tissue is found to be difficult to section. The tissue is soft and mushy. To correct this in the future, change the processing schedule by: *
a. Decreasing time in formalin
b. Increasing time in dehydrant
c. Decreasing time in clearant
d. Decreasing time in paraffin
b. Increasing time in dehydrant
Prolonged dehydration in higher grades of alcohol will render the specimen:
a. Hard
b. Macerated
c. Porous
d. toxic
a. Hard
Why should the alcohols on the automated tissue processor be changed on a regular basis?
a. The alcohols become saturated with bile from the gallbladder specimens, which can be absorbed by the other tissues.
b. Too great a concentration of formalin in the alcohols can create a potentially explosive situation.
c. Gram negative organisms can grow in alcohol left too long on the processor.
d. The alcohols can absorb moisture and become dilute.
d. The alcohols can absorb moisture and become dilute.
Which of the following will speed up the rate of decalcification, yet not cause harm to the tissue?
a. Increase the percentage of acid
b. Decrease the temperature of the decal fluid
c. Agitate the solution
d. Gross a thicker specimen.
c. Agitate the solution
The maximum thickness of tissue which will allow adequate clearing in xylene in routine processing is:
a. 1-2 mm
b. 3-4 mm
c. 5-6 mm
d. 7-8 mm
b. 3-4 mm
Which of the following are used as clearants only?
a. Acetone, Methyl salicylate, Toluene
b. Amy1 acetate, Methyl benzoate, Tetrahydrofuran
c. Benzene, Carbon tetrachloride, Cedarwood oil
d. Chloroform, Isopropanol, Xylene
c. Benzene, Carbon tetrachloride, Cedarwood oil
Which of the following is considered the best routine dehydrant?
a. Acetone
b. Ethanol
c. Isopropanol
d. Methanol
b. Ethanol
The process of saturating tissue with the medium that will later be used for embedding is called:
a. Clearing
b. Dehydration
c. Fixation
d. infiltration
d. infiltration
