6/7. GPCRs

Question 1

What is another Gs family member?

Answer 1

G olf

Question 2

What does Gs do?

Answer 2

stimulatory - activates adenylate cyclase

Question 3

4 other Gi family members?

Answer 3

Go Gt Gg Gz

Question 4

Gi…

Answer 4

… inhibits adenylate cyclase

Question 5

Gq members? (4)

Answer 5

G11 G14 G15 G16

Question 6

What does Gq do?

Answer 6

Phospholipase C beta activation

Question 7

How is phototransduction an example of signal amplification?

Answer 7

1 receptor : rhodopsin
10^2 mediators: transducin
10^3 effectors: cGMP phosphodiesterase
10^6 second messengers: cGMP

Question 8

How do cardiomyocytes show signal integration?

Answer 8

beta-adrenergic receptors stimulate adenylate cyclase whilst alpha-adrenergic receptors inhibit it.
beta works through Gs protein
alpha through Gi

Question 9

Examples of ligands that bind to family 1? 3

Answer 9

dopamine, rhodopsin, vasopressin

Question 10

Family 2 ligands?

Answer 10

Glucagon, glucagon-like-peptide 1

Question 11

Paper which suggests that RGS proteins interact with GPCRs?

Answer 11

Croft et al 2013

Question 12

Name 5 different GPCR interacting proteins?

Answer 12

RGS, heterotrimeric G proteins, other GPCRs, kinases, beta arrestins