501-600 Flashcards
If more than 500 platelets are counted on each side, a ___ dilution should be made.
A. 1:10
B. 1:20
C. 1:100
D. 1:200
D. 1:200
For manual WBC count, allow the dilution to sit for ________ to ensure that the red blood cells have lysed.
A. 3 minutes
B. 5 minutes
C. 10 minutes
D. 15 minutes
C. 10 minutes
For manual WBC count, after charging the hemacytometer, place it in a moist chamber for _______ before counting the cells to give them time to settle.
A. 3 minutes
B. 5 minutes
C. 10 minutes
D. 15 minutes
C. 10 minutes
For manual platelet count, place the charged hemacytometer in a moist chamber for _________ to allow the platelets to settle.
A. 3 minutes
B. 5 minutes
C. 10 minutes
D. 15 minutes
D. 15 minutes
Culture media for Vibriospp., EXCEPT:
A. Buffered glycerol saline
B. Cary-Blair
C. Alkaline peptone water
D. Thiosulfate citrate bile salt (TCBS) agar
A. Buffered glycerol saline
Which is the most appropriate nonselective medium for recovery of mycobacteria from a heavily contaminated specimen?
A. Löwenstein–Jensen agar
B. Middlebrook 7H10 agar
C. Petragnani’s agar
D. American Thoracic Society medium
C. Petragnani’s agar
Fluorescence on Wood’s lamp, growth in rice medium:
A. Microsporum canis
B. Microsporum gypseum
C. Microsporum audouinii
D. Epidermophyton floccosum
A. Microsporum canis
Fluorescence on Wood’s lamp, no growth in rice medium:
A. Microsporum canis
B. Microsporum gypseum
C. Microsporum audouinii
D. Epidermophyton floccosum
C. Microsporum audouinii
It is a serious pathogen of the eye, causing progressive
endophthalmitis:
A. Bacillus anthracis
B. Bacillus cereus
C. Bacillus subtilis
D. Bacillus thurigiensis
B. Bacillus cereus
Added to SDA to inhibit most contaminating bacteria:
A. Dextrose
B. Chloramphenicol
C. Cycloheximide
D. Nystatin
B. Chloramphenicol
Added to SDA to inhibit saprophytic fungi:
A. Dextrose
B. Chloramphenicol
C. Cycloheximide
D. Nystatin
C. Cycloheximide
Which Brucella species may require CO2 for growth, is urea positive in 2 hours, and is inhibited by thionine dye?
A. Brucella abortus
B. Brucella melitensis
C. Brucella suis
D. Brucella canis
A. Brucella abortus
Brucella isolate that does not produce H2S, does not require CO2, and is not inhibited by thionine and basic fuchsin is probably which species of Brucella?
A. Brucella canis
B. Brucella abortus
C. Brucella suis
D. Brucella melitensis
D. Brucella melitensis
Which test is used for the determination of inducible clindamycin resistance in staphylococci and streptococci?
A. E-test
B. D-zone test
C. A-test
D. CAMP test
B. D-zone test
The D-zone susceptibility test is used to test inducible resistance on S. aureus strains demonstrating an initial antibiotic susceptibility profile of
A. Erythromycin sensitive, clindamycin sensitive
B. Erythromycin resistant, clindamycin sensitive
C. Erythromycin resistant, clindamycin resistant
D. Erythromycin sensitive, clindamycin resistant
B. Erythromycin resistant, clindamycin sensitive
Resistance to clindamycin can be induced in vitro by
A. Ampicillin
B. Erythromycin
C. Gentamicin
D. Penicillin
B. Erythromycin
Staphylococcus aureus toxin which disrupts the smooth muscle in blood vessels and is toxic to erythrocytes, leukocytes, hepatocytes, and platelets:
A. Alpha toxin
B. Beta toxin
C. Delta toxin
D. Gamma toxin
A. Alpha toxin
An isolate of Staphylococcus aureus was cultured from an ulcer obtained from the leg of a diabetic 79-year-old female patient. The organism showed resistance to methicillin. Additionally, this isolate should be tested for resistance or susceptibility to:
A. Erythromycin
B. Gentamicin
C. Vancomycin
D. Kanamycin
C. Vancomycin
Access to the laboratory is limited or restricted, and there must be a biohazard sign posted at the entrance of the laboratory:
A. BSL-1
B. BSL-2
C. BSL-3
D. BSL-4
A. BSL-1
Access to the laboratory is limited when work is being conducted. The laboratory director is ultimately responsible for determining who may enter or work in the laboratory.
A. BSL-1
B. BSL-2
C. BSL-3
D. BSL-4
B. BSL-2
Laboratory should be separated from the other parts of the building and be accessed through two self-closing doors. An ANTEROOM may be used for access.
A. BSL-1
B. BSL-2
C. BSL-3
D. BSL-4
C. BSL-3
The BSL facility either is located in a separate building or is in an isolated zone within a building.
A. BSL-1
B. BSL-2
C. BSL-3
D. BSL-4
D. BSL-4
A catalase-negative, gram-positive coccus resembling staphylococci (clusters on the Gram- stained smear) was recovered from three di erent blood cultures obtained from a 60-year-old patient diagnosed with endocarditis. The following test results were noted:
PYR = Neg
Esculin hydrolysis = Neg
Vancomycin = Sensitive
LAP = Neg (V)
6.5% Salt broth = Neg CAMP test = Neg
What is the correct identification?
A. Leuconostoc spp.
B. Gemella spp.
C. Enterococcus spp.
D. Micrococcus spp
B. Gemella spp.
pH indicator incorporated in xylose-lysine-desoxycholate (XLD) agar:
A. Bromcresol purple
B. Bromthymol blue
C. Neutral red
D. Phenol red
D. Phenol red
SAFEST method to ensure that NO INFECTIVE MATERIALS REMAIN in samples or containers when disposed:
A. Autoclave
B. Incineration
C. Pasteurization
D. Filtration
B. Incineration
Unopened, sliced BACON, packaged in material somewhat highly resistant to oxygen permeability, is spoiled mostly by
A. Lactobacilli
B. Micrococci
C. Fecal Streptococci
D. Molds
A. Lactobacilli
ANAEROBES usually cannot grow in the presence of O2, and the atmosphere in anaerobe jars, bags, or chambers is composed of:
A. 5% to 10% H2, 5% to 10% CO2, 80% to 90% O2, and 0% N2
B. 5% to 10% H2, 5% to 10% CO2, 80% to 90% N2, and 0% O2
C. 5% to 10% N2, 5% to 10% CO2, 80% to 90% H2, and 0% O2
D. 5% to 10% H2, 5% to 10% N2, 80% to 90% CO2, and 0% O2
B. 5% to 10% H2, 5% to 10% CO2, 80% to 90% N2, and 0% O2
What is the stain that binds to the nucleic acid of organisms but does not discriminate between gram-positive or gram-negative organisms called?
A. Ziehl-Neelsen stain
B. Auramine-rhodamine stain
C. Gram stain
D. Acridine orange stain
D. Acridine orange stain
The most important diagnostic tool in treating patients with clinical infections in the emergency department:
A. AFB stain
B. Culture
C. Gram stain
D. IMVic reaction
C. Gram stain
Centrifugation for urine microscopic examination:
A. RCF of 100 for 2 minutes
B. RCF of 400 for 5 minutes
C. RCF of 500 for 5 minutes
D. RCF of 500 for 10 minutes
B. RCF of 400 for 5 minutes
A white blood cell (WBC) count and WBC di erential are performed. WBC count: 7.0 × 10^9/L; of 100 WBCs classified:
● 70% neutrophils
● 20% lymphocytes
● 7% monocytes
● 2% eosinophils
● 1% basophil
The absolute neutrophil cell count is:
A. 2.10×10^9/L
B. 3.55×10^9/L
C. 3.99×10^9/L
D. 4.9×10^9/L
D. 4.9×10^9/L
A patient being treated for metastatic carcinoma was found to have a white cell count of 5×10^9/L with 5 metarubricytes (nucleated red cells) per 100 white WBCs. What is the corrected white cell count for this patient?
A. 2.1 × 10^9/L
B. 2.4 × 10^9/L
C. 4.8 × 10^9/L
D. 5.2 × 10^9/L
C. 4.8 × 10^9/L
The concentration of human chorionic gonadotropin (hCG) is generally at a particular level in serum about 2 to 3 days after implantation. This is the concentration at which most sensitive laboratory assays can give a positive serum hCG result. What is the lowest level of hormone for which most current serum hCG tests can give a positive result?
A. 25 mIU/mL
B. 50 mIU/mL
C. 100 mIU/mL
D. 100,000 mIU/mL
A. 25 mIU/mL
Using serum electrophoresis, protein can be separated into how many fractions?
A. Three
B. Four
C. Five
D. Six
C. Five
Laseris an acronym for:
A. Light amplification by stimulated emission of radiation
B. Light augmentation by stimulated emission of radiation
C. Light amplification of stimulated energy radiation
D. Large-angle stimulated emission of radiation
A. Light amplification by stimulated emission of radiation
How would 6.32 be rounded o to one less decimal place?
A. 6.32
B. 6.4
C. 7.0
D. 6.3
D. 6.3
How would 15.57 be rounded o to one less decimal place?
A. 15.6
B. 15.5
C. 16.0
D. 15.0
A. 15.6
The purest type of reagent water is:
A. Type I
B. Type II
C. Type III
D. All are equal
A. Type I
In flow cytometry, the term “gating” refers to:
A. Selection of a subpopulation of cells to count
B. Determining the fluorescent emission spectrum of cells of interest
C. Interference caused by binding of more than a single antibody
D. Selecting the appropriate counting aperture
A. Selection of a subpopulation of cells to count
Major systems in a flow cytometer include all of the following except:
A. Fluidics
B. Optics
C. Computerized electronics
D. Gating
D. Gating