5-6 MARKERS Flashcards
Compare and contrast the structure and properties of triglycerides and phospholipids.
COMPARE:
• Both have ester bonds and contain glycerol
• Fatty acids may both be saturated or unsaturated
• Both are insoluble in water
• Both contain C, H and O
CONTRAST
• Triglyceride has 3 fatty acids whereas phospholipids have 2 fatty acids plus PO43- group.
• Triglycerides are hydrophobic/non-polar whereas phospholipids have both a hydrophilic and hydrophobic region
• Phospholipids form monolayer/micelle/bilayer whereas triglycerides don’t.
Describe how the structures of starch and cellulose molecules are related to their functions. (5)
Starch (max 3)
1. Helical/ spiral shape so compact;
2. Large (molecule)/insoluble so osmotically inactive;
3. Branched so glucose is (easily) released for respiration;
4. Large (molecule) so cannot leave cell/cross cell-surface membrane;
Cellulose (max 3)
5. Long, straight/unbranched chains of β glucose;
6. Joined by hydrogen bonding;
7. To form (micro/macro)fibrils;
8. Provides rigidity/strength;
Explain why the diffusion of chloride ions involves a membrane protein and the diffusion of oxygen does not. (5)
- Chloride ions water soluble/charged/polar;
- Cannot cross (lipid) bilayer (of membrane);
- Chloride ions transported by facilitated diffusion OR diffusion involving channel/carrier protein;
- Oxygen not charged/non-polar;
- (Oxygen) soluble in/can diffuse across (lipid) bilayer;
Use your knowledge of protein structure to explain why enzymes are specific and may be affected by non-competitive inhibitors. (5)
1 each enzyme / protein has specific primary structure / amino acid sequence;
2 folds in a particular way / has particular tertiary structure giving an active site with a unique structure;
3 shape of active site complementary to / will only fit that of substrate;
4 inhibitor fits at site on the enzyme other than active site;
5 distorts active site;
6 so substrate will no longer fit / form enzyme-substrate complex
Describe the role of the enzymes of the digestive system in the complete breakdown of starch. (5)
Amylase;
(Starch) to maltose:
Maltase;
Maltose to glucose;
Hydrolysis;
(Of) glycosidic bond;
Describe the role of the enzymes of the digestive system in the complete breakdown of starch. (5)
Amylase;
(Starch) to maltose:
Maltase;
Maltose to glucose;
Hydrolysis;
(Of) glycosidic bond;
In humans, the enzyme maltase breaks down maltose to glucose. This takes place at normal body temperature.
Explain why maltase:
-only breaks down maltose
-allows this reaction to take place at normal body temperature. (5)
- Tertiary structure / 3D shape of enzyme (means);
- Active site complementary to maltose / substrate / maltose fits into active site / active site and substrate fit like a lock and key;
- Description of induced fit;
- Enzyme is a catalyst;
- Lowers activation energy / energy required for reaction;
- By forming enzyme-substrate complex;
Scientists have investigated the effects of competitive and non-competitive inhibitors of the enzyme maltase.
Describe competitive and non-competitive inhibition of an enzyme. (5)
- Inhibitors reduce binding of enzyme to substrate / prevent formation of ES complex;
(Competitive inhibition), - Inhibitor similar shape (idea) to substrate;
- (binds) in to active site (of enzyme);
- (Inhibition) can be overcome by more substrate;
(Non-competitive inhibition), - Inhibitor binds to site on enzyme other than active site;
- Prevents formation of active site / changes (shape of) active site;
- Cannot be overcome by adding more substrate;
Explain how the structure of DNA is related to its functions. (6)
- Sugar-phosphate (backbone)/double
stranded/helix so provides strength/stability
/protects bases/protects hydrogen bonds; - Long/large molecule so can store lots of
information; - Helix/coiled so compact;
- Base sequence allows information to be
stored/ base sequence codes for amino
acids/protein; - Double stranded so replication can occur
semi-conservatively/ strands can act as
templates; - Complementary base pairing / A-T and G-C
so accurate replication/identical copies can
be made; - (Weak) hydrogen bonds for replication/
unzipping/strand separation; - Many hydrogen bonds so stable/strong;
Atheroma formation increases a person’s risk of dying. Explain how. (5)
- Atheroma is fatty material/cholesterol/foam cells/plaque/calcium deposits/LDL;
- In wall of artery;
- (Higher risk of) aneurysm/described;
- (Higher risk of) thrombus formation/blood clot;
- Blocks coronary artery;
- Less oxygen/glucose to heart muscle/cells/tissue;
- Reduces/prevents respiration;
- Causing myocardial infarction/heart attack;
- Blocks artery to brain;
- Causes stroke/stroke described;
Describe how DNA is replicated. (6)
- Strands separate / H-bonds break;
- DNA helicase (involved);
- Both strands/each strand act(s) as (a) template(s);
- (Free) nucleotides attach;
- Complementary/specific base pairing / AT and GC;
- DNA polymerase joins nucleotides (on new strand);
- H-bonds reform;
- Semi-conservative replication / new DNA molecules contain one old strand and one new strand;
Describe how tissue fluid is formed and how it is returned to the circulatory system. (6)
Formation
1. High blood / hydrostatic pressure /
pressure filtration;
2. Forces water / fluid out;
3. Large proteins remain in capillary;
Return
4. Low water potential in capillary /
blood;
5. Due to (plasma) proteins;
6. Water enters capillary / blood;
7. (By) osmosis;
8. Correct reference to lymph;
Some substances can cross the cell-surface membrane of a cell by simple diffusion through the phospholipid bilayer.
Describe other ways by which substances cross this membrane. (5)
By osmosis (no mark)
1. From a high water potential to a low water potential/down a water potential gradient;
2. Through aquaporins/water channels;
By facilitated diffusion (no mark)
3. Channel/carrier protein;
4. Down concentration gradient;
By active transport (no mark)
5. Carrier protein/protein pumps;
6. Against concentration gradient;
7. Using ATP/energy (from respiration);
By phagocytosis/endocytosis (no mark)
8. Engulfing by cell surface membrane to form vesicle/vacuole;
By exocytosis/role of Golgi vesicles (no mark)
9. Fusion of vesicle with cell surface membrane;
The events that take place during interphase and mitosis lead to the production of two genetically identical cells. Explain how. (4)
- DNA replicated;
- (Involving)
specific/accurate/complementary
base-pairing; - (Ref to) two identical/sister
chromatids; - Each chromatid/ moves/is separated
to(opposite) poles/ends of cell;
A mutation can lead to the production of a non-functional enzyme. Explain how. (6)
- Change/mutation in base/nucleotide
sequence (of DNA/gene); - Change in amino acid
sequence/primary structure (of
enzyme); - Change in hydrogen/ionic/disulfide
bonds; - Change in the tertiary
structure/shape; - Change in active site;
- Substrate not complementary/cannot
bind (to enzyme/active site) / no
enzyme-substrate complexes form;