4. Genital Tract Infections

Question 1

What ingredient in GBS broth inhibits most Gram-negative organisms?

Answer 1

Colistin sulfate

Question 2

Which population of women?

Mainly Corynebacterium species and coagulase negative staphylococci

Answer 2

pre-pubescent girls

Question 3

This disease causes a painless, hard chancre to form

Answer 3

Syphillis

Question 4

What ingredient in LIM Broth inhibits most gram negative organisms?

Answer 4

Colistin sulfate

Question 5

Specimen collected to screen for prenatal GBS

Answer 5

Vag/rectal swab (ano-vaginal swab)

Question 6

Treponema pallidum cuses what disease?

Answer 6

Syphillis

Question 7

Which disease causes a painful, soft chancre? Painless, hard chancre?

Answer 7

Soft + painful = Chancroid

Hard + painless = Syphilis

Question 8

Causes neonatal pneumonia during birth

Answer 8

Chlamydia

Question 9

Why is MODIFIED Martin-Lewis agar used to isolate pathogenic Neisseria?

Answer 9

It has a lower concentration of vancomycin than regular ML agar to reduce inhibition of vancomycin-sensitive N. gonorrhoeae

Question 10

Which of the following cause vaginitis?

a) Neisseria gonorrhoeae
b) Gardnerella vaginalis
c) Candida albicans
d) Lactobacillus species

Answer 10

c) Candida albicans

Gardnerella is ASSOCIATED w/ BV but it does not cause it

Question 11

What does a VDRL lab test detect?

Answer 11

Treponema pallidum (syphillis causative agent)

Question 12

A vaginal swab is received for GBS, screening. How should the technologist proceed?

Answer 12

Process the specimen and add a comment to the report about the suboptimal specimen

Question 13

Which population of women?

Along with Corynebacterium species, G. vaginalis, Mobiluncus species, coagulase negative staphylococci and small amounts of yeast, lactobacilli predominate.

Answer 13

women in their reproductive years

Question 14

Name of INFECTIVE form of Chlamydia?

Answer 14

Elementary body (EB)

Question 15

This antibiotic is given intramuscularly for treatment of gonorrhea

Answer 15

Ceftriaxone (extended spectrum cephalosporin)

Question 16

Most common causes of neonatal meningitis during birth

Answer 16

• GBS
• Listeria
• E. coli

Question 17

Which of the following best describes Neisseria gonorrhoeae?

Oxidase positive, ONPG positive, glucose positive, DNAse positive

Oxidase negative, glucose positive, maltose positive, growth on nutrient agar

Oxidase positive, glucose positive, no growth on nutrient agar, DNAse negative

Glucose positive, DNAse negative, ONPG positive, growth on nutrient agar

Answer 17

c) Oxidase (+)
Glucose (+)
NG on nutrient agar
DNAse (-)

Question 18

Causative agent of genital warts

Answer 18

Human papilloma virus (HPV).

Question 19

Haemophilus ducreyi is the causative agent of what disease?

Answer 19

Chancroid

Question 20

Name of REPLICATE form of Chlamydia?

Answer 20

Reticulate bodies (RB)

Question 21

What is the best method for overnight transport of a sample collected from a cervix?

a) A rayon swab in Amies medium
b) A cotton swab in charcoal medium
c) A JEMBEC transport system
d) An anaerobic swab

Answer 21

c) A JEMBEC transport system
* Has its own CO2 generating system = best for isolation of gonococci when there is a delay between collection and arrival in the lab*

Question 22

Which population of women?

Increased staphylococci and coliforms, fewer lactobacilli.

Answer 22

post-menopausal women

Question 23

What feature of Trichomonas vaginalis aids in its detection from vaginal secretions?

Answer 23

It has an undulating membrane that extends half the length of its body

It is motility that makes T. vaginalis easily identifiable, so both the flagella and undulating membrane contribute.

Question 24

Main causative agent of genital herpes?

Answer 24

Herpes simplex 2 (HSV-2)