4. Genital Tract Infections Flashcards
What ingredient in GBS broth inhibits most Gram-negative organisms?
Colistin sulfate
Which population of women?
Mainly Corynebacterium species and coagulase negative staphylococci
pre-pubescent girls
This disease causes a painless, hard chancre to form
Syphillis
What ingredient in LIM Broth inhibits most gram negative organisms?
Colistin sulfate
Specimen collected to screen for prenatal GBS
Vag/rectal swab (ano-vaginal swab)
Treponema pallidum cuses what disease?
Syphillis
Which disease causes a painful, soft chancre? Painless, hard chancre?
Soft + painful = Chancroid
Hard + painless = Syphilis
Causes neonatal pneumonia during birth
Chlamydia
Why is MODIFIED Martin-Lewis agar used to isolate pathogenic Neisseria?
It has a lower concentration of vancomycin than regular ML agar to reduce inhibition of vancomycin-sensitive N. gonorrhoeae
Which of the following cause vaginitis?
a) Neisseria gonorrhoeae
b) Gardnerella vaginalis
c) Candida albicans
d) Lactobacillus species
c) Candida albicans
Gardnerella is ASSOCIATED w/ BV but it does not cause it
What does a VDRL lab test detect?
Treponema pallidum (syphillis causative agent)
A vaginal swab is received for GBS, screening. How should the technologist proceed?
Process the specimen and add a comment to the report about the suboptimal specimen
Which population of women?
Along with Corynebacterium species, G. vaginalis, Mobiluncus species, coagulase negative staphylococci and small amounts of yeast, lactobacilli predominate.
women in their reproductive years
Name of INFECTIVE form of Chlamydia?
Elementary body (EB)
This antibiotic is given intramuscularly for treatment of gonorrhea
Ceftriaxone (extended spectrum cephalosporin)
Most common causes of neonatal meningitis during birth
- GBS
- Listeria
- E. coli
Which of the following best describes Neisseria gonorrhoeae?
Oxidase positive, ONPG positive, glucose positive, DNAse positive
Oxidase negative, glucose positive, maltose positive, growth on nutrient agar
Oxidase positive, glucose positive, no growth on nutrient agar, DNAse negative
Glucose positive, DNAse negative, ONPG positive, growth on nutrient agar
c) Oxidase (+)
Glucose (+)
NG on nutrient agar
DNAse (-)
Causative agent of genital warts
Human papilloma virus (HPV).
Haemophilus ducreyi is the causative agent of what disease?
Chancroid
Name of REPLICATE form of Chlamydia?
Reticulate bodies (RB)
What is the best method for overnight transport of a sample collected from a cervix?
a) A rayon swab in Amies medium
b) A cotton swab in charcoal medium
c) A JEMBEC transport system
d) An anaerobic swab
c) A JEMBEC transport system
* Has its own CO2 generating system = best for isolation of gonococci when there is a delay between collection and arrival in the lab*
Which population of women?
Increased staphylococci and coliforms, fewer lactobacilli.
post-menopausal women
What feature of Trichomonas vaginalis aids in its detection from vaginal secretions?
It has an undulating membrane that extends half the length of its body
It is motility that makes T. vaginalis easily identifiable, so both the flagella and undulating membrane contribute.
Main causative agent of genital herpes?
Herpes simplex 2 (HSV-2)
