Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Clinical Microbiology 2 > 3. Gastrointestinal Infections > Flashcards

3. Gastrointestinal Infections Flashcards

You may prefer our related Brainscape-certified flashcards:
Biology 101 flashcards
1
Q

Purpose of buffered glycerol

A

Transport best for Shigella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Infections due to this organism are NOT treated empirically

A

Shigella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Why is the serotyping of stool pathogens not performed on growth from a MacConkey agar plate?

a) Dyes in the medium (ie., crystal violet and neutral red) can cause a false reaction
b) Carbohydrates in the medium can cause autoagglutination
c) Because you need to do an oxidase test at the same time
d) It is difficult to get isolated colonies on a MAC plate.

A

b) Carbohydrates in the medium can cause autoagglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Humans and large primates are the only reservoir of this organism

A

Shigella

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Name of watery diarrhea w/ fleck of white-ish material (mucous + GI epithelial cells)

Which organism causes it?

A

Rice water stools

Vibrio cholera

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

E. coli acronym that causes HUS

A

EHEC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

AKA Shiga-toxin producing E. coli

A

EHEC (STEC)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

If a bloody stool is received, an aliquot is always sent for investigation for other pathogenic toxin-producing E. coli where?

A
  • BCCDC

- On site if molecular techniques available

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Liquid media suitable for H. pylori?

A

Urea broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Pathogen detected by TCBS

A

Vibrio

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Green colony on TCBS = which organism?

A

Vibrio parahemolyticus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Yellow colony on TCBS = which organism?

A

Vibrio cholerae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is Reiter’s syndrome

A

Reactive arthritis caused by Campy as well as Chlamydia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Pathogen detected by XLD agar

A

Shigella spp, Salmonella spp.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Which of the following best describes Shigella sp.?

a) ONPG(-), glucose(-), lysine(+), motility(-)
b) ONPG(+), glucose(+), lysine(-), motility(-)
c) ONPG(-), glucose(+), lysine(-), motility(+)
d) ONPG(-), glucose(+), lysine(-), motility(-)

A

d) ONPG(-), glucose(+), lysine(-), motility(-)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

2 viruses that cause diarrhea

A
  • Rotavirus

- Norwalk virus

17
Q

A two year-old child is hospitalized for dehydration from severe diarrhea and vomiting. What is the most likely etiological agent?

a) Clostridium difficile
b) Rotavirus
c) Vibrio cholerae
d) Hepatitis A

A

b) Rotavirus

* This virus is an important worldwide cause of diarrhea in infants and young children.*

18
Q

Stool transport media that can be stored/transported at RT

A

Cary-Blair transport medium

19
Q

Which ingredient in Hektoen agar would be considered differential?

Bile salts

Yeast extract

Ferric chloride

Salicin

A

Salicin

Three carbohydrates are present to differentiate between different enteric organisms; lactose, sucrose, and salicin.

20
Q

E. coli acronym that causes traveler’s diarrhea

A

ETEC

21
Q

Pathogen detected by CIN agar

A

Yersinia

22
Q

Most common serotype of Shigella in North America

A

Sh. sonnei (Group D)

23
Q

E. coli acronym that is not invasive and produces no toxin

A

EPEC

24
Q

PVA preservative cannot be used in the investigation of this organism

A

Cryptosporidium

25
Q

What is detected by the cytotoxin assay for Clostridium difficile?

a) Toxin A and toxin B
b) Toxin A only
c) Toxin B only
d) Toxins A, B and C

A

c) Toxin B only
- The cytotoxin assay detects toxin B.
- EIA and latex tests detect toxins A and/or B.

26
Q

AST usually tested but not reported for this organism

A

Salmonella spp.

27
Q

Which of the following tests best differentiates Salmonella sp.from Citrobacter sp.?

H2S production

Phenylalanine deaminase

Lysine decarboxylation

Citrate utilization

A

Lysine decarboxylation

This test differentiates these organisms since Salmonella sp. are lysine positive and Citrobacter sp. are negative.

28
Q

Which of the following is true of Yersinia enterocolitica?

a) It’s motile at room temperatures, but not at 37°C
b) It’s the causative agent of bubonic plague
c) It utilizes citrate
d) It grows best at 42°C

A

a) It’s motile at room temperatures, but not at 37°C

* All Yersinia species, other than Y. pestis, are motile at lower temperatures (22-28ºC), but is non-motile at 37ºC.*

29
Q

If Salmonella only has agglutination with the Vi antiserum, what should be done?

A

Boil for 10 mins, re-test the polyvalent O antiserum

30
Q

Campylobacter catalase result?

A

Catalase POS

31
Q

Characteristic that separates Plesiomonas from the rest of the Enterobacteriaceae family

A

Oxidase POS

32
Q

What is SAF preservative in Ova and Parasite collection?

A

Sodium acetate, acetic acid, formalin

33
Q

What is PBS broth used for

A

Yersinia cold enrichment

34
Q

Rarest and most virulent Shigella

A

Sh. dysenteriae (Group A)

35
Q

Which of the following organisms must be reported to Public Health authorities in British Columbia?

Campylobacter species

Salmonella species

Yersinia species

All of the above

A

All of the above

All specific bacterial and viral stool pathogens are reportable to Public Health in British Columbia

36
Q

pH indicator in TSI

A

Phenol red

37
Q

When a stool specimen is collected for bacterial culture and will not be delivered to the lab for at least 4 hours after collection what is the best method for transport?

In a clean, dry container

On a swab dipped into the specimen

In Cary-Blair transport medium

In sodium acetate – acetic acid – formalin

A

In Cary-Blair transport medium

This medium will allow the recovery of most stool pathogens and it is required anytime transport to the lab will be greater than 2 hours.

38
Q

What is the purpose of using an enrichment media in stool cultures?

A

Suppress growth of coliforms while allowing Salmonella and Shigella to grow

39
Q

3 parasites that cause diarrhea

A
  • Giardia lambia
  • Cryptosporidium spp.
  • Entamoeba histolytica

Clinical Microbiology 2 (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions