38. Clinical Enzymology

Question 1

What is clinical biochemistry?

Answer 1

Using chemical and biochemical techniques to study disease and assist patient care

Question 2

What does high ALT suggest?

Answer 2

hepatocellular damage

Question 3

What does high ALP suggest?

Answer 3

cholestatic liver disease

bone disease

Question 4

What does high AST suggest?

Answer 4

hepatocellular damage

muscle damage

Question 5

What does high CK suggest?

Answer 5

Muscle damage

Acute MI

Question 6

What does high LDH suggest?

Answer 6

Muscle damage

Blood cell breakdown

Question 7

What is nephelometry?

Answer 7

Measures scattered light

At an angle

Question 8

What is turbidimetry?

Answer 8

Measures light that hasn’t been scattered

In direct path of light

Question 9

What are the two types of quality control?

Answer 9

Internal quality control: several times a day with calibrators
External quality assurance: monthly, same sample sent to many labs to compare them

Question 10

What is a unit of enzyme activity?

Answer 10

Quantity of an enzyme that catalyses the reaction of 1 mmol of substrate per minute

Question 11

What are isoforms?

Answer 11

Forms of enzyme created through posttranslational modifications of enzyme models

Question 12

What does high GGT suggest?

Answer 12

Liver disease

Question 13

What do high amylase and lipase suggest?

Answer 13

Acute pancreatitis

Question 14

What is an LFT?

Answer 14

Liver function test