3.1 Methods of studying cells

Question 1

What is the formula for magnification?

Answer 1

magnification = size of image/size of real

Question 2

What is RESOLUTION?

Answer 2

the ability to distinguish between two objects that are very close together

Question 3

What is the resolution and magnification of a light microscope?

Answer 3

R = 200nm
M = 1.500x

Question 4

What is the resolution and magnification of a TEM?

Answer 4

R = 0.2nm (highest)
M = 1,000,000 < x

Question 5

What is the resolution and magnification of an SEM?

Answer 5

R = 1nm
M = <500,000 x

Question 6

How do you prepare cell fractionation?

Answer 6

• Cold, to reduce enzyme activity
• Same water potential as tissue to prevent organelles from bursting or shrinking
• Buffered so that the pH doesn’t fluctuate