2S [LEC]: Compatibility Testing Flashcards
Major cause of transfusion-related fatalities, resulting in incorrect ABO groupings and transfusion of ABO incompatible blood
Clerical error (Patient identification)
T/F: Either anticoagulated or clotted specimens are acceptable for pretransfusion testing.
True
In pregnant patients, the specimens must be less than ___ old if the patient was pregnant or received RBC products by transfusion within the past 3 months
3 day
The donor and recipient samples must be stored for a minimum of ___ days following transfusion.
7
Most critical pretransfusion serologic test
ABO grouping
T/F: The test for Du is necessary when testing recipients.
False
T/F: The test for Du is necessary when testing recipients.
False
Done for Final checking of ABO compatibility between patient and donor to prevent transfusion reaction.
Crossmatching
Detects presence of antibody in patient’s serum that will react to donor’s RBC that is not detected in antibody screen.
Crossmatching
Refers to Abs that are reactive at 37ºC and/or in the AHG test and are known to have caused a transfusion reaction or unacceptably short survival of the transfused red cells
Clinically significant Abs
T/F: The Kidd blood group is always regarded as being potentially clinically significant
False (ABO, Rh, Kell, Duffy, Kidd, SsU)
Traditional method of antibody screening
Tube method
Reduces the zeta potential; disperses the charges, allows RBCs to approach each other and increases the chances of agglutination
22% Albumin
Contains glycine in an albumin solution; lowers the zeta potential; increased the uptake of ab onto the RBC during sensitization
LISS
Removes water in LISS and concentrates the Abs; increases the degree of sensitization; causes non-specific aggregation
PEG
Allows agglutination of INCOMPLETE abs
AHG rgts
Cleave the disulfide bonds of IgM molecules and help differentiate between IgM and IgG antibodies
Sulfhydryl rgts
Good to use when you have an IgM and need to identify underlying IgG, usually after enzyme treatment
Sulfhydryl rgts
A combination of proteolytic enzymes (papain) and DTT
ZZAP
ZZAP does not denature which blood antigen?
Kx antigen
Destroys Kell, M, N, S, Duffy and other less frequent blood group antigens
ZZAP
Chemicals dissociate IgG from RBCs and render DAT+ red cells DAT-
CDP and Glycine acid EDTA
Destroys Kell, Cartwright (Cw), Er, Bg
Glycine Acid EDTA
Denatures HLA antigens (Bg) on RBCs
Chloroquine diphosphate
